不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应

doi:10.12302/j.issn.1000-2006.202204059

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南京林业大学学报（自然科学版） ›› 2024, Vol. 48 ›› Issue (3): 172-180.doi: 10.12302/j.issn.1000-2006.202204059

不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应

马坛¹(), 田野¹^,^*(), 王书军², 李文昊¹, 段启英¹, 张庆源¹

1.南京林业大学林草学院,南方现代林业协同创新中心,江苏南京 210037
2.江苏省宝应县育兴苗木场,江苏宝应 225831

收稿日期:2022-04-25 修回日期:2023-06-28 出版日期:2024-05-30 发布日期:2024-06-14
通讯作者: *田野(tianye@njfu.edu.cn; tianyes@hotmail.com),副教授。
作者简介:马坛(1197411729@qq.com)。
基金资助:
国家重点研发计划(2021YFD2201202);宝应县重点研发计划(现代农业)(BY202115)

Sex-specific leaf physiological responses of southern-type poplar to short-term intermittent soil drought

MA Tan¹(), TIAN Ye¹^,^*(), WANG Shujun², LI Wenhao¹, DUAN Qiying¹, ZHANG Qingyuan¹

1. Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry and Grass, Nanjing Forestry University, Nanjing 210037, China
2. Yuxing Nursery Farm, Baoying 225831, China

Received:2022-04-25 Revised:2023-06-28 Online:2024-05-30 Published:2024-06-14

摘要/Abstract

摘要：

【目的】全球气候变化背景下南方型黑杨(Populus spp.)栽培区的干旱问题日渐严重,限制了人工林的生产力提升。研究南方型黑杨应对土壤短期间歇性干旱的性别生理响应差异,探讨不同性别黑杨的干旱适应能力及策略,为基于性别的黑杨造林无性系选择提供参考。【方法】以南方型黑杨栽培区重点推广的4种雌性和3种雄性黑杨无性系当年生扦插苗为研究对象,采用盆栽控水的方式进行土壤短期间歇性干旱(干旱—复水—干旱)处理,通过对比分析不同性别无性系在土壤短期间歇性干旱过程中叶片质膜稳定性、渗透调节、抗氧化系统,以及光合色素等生理指标的变化,分析南方型黑杨在叶片生理响应和干旱耐受性方面应对土壤短期间歇性干旱的性别差异。【结果】受土壤干旱影响,雌、雄黑杨叶片均出现电导率上升、丙二醛和游离脯氨酸含量以及抗氧化酶活性升高、叶绿素含量下降等现象,表明叶片膜和光合系统受到一定损伤,而复水(解除干旱)后均恢复至正常水平。但雌、雄黑杨在生理响应的水平和模式方面存在一定差异:雌性黑杨在干旱胁迫时叶片抗氧化酶活性波动较大,叶绿素和脯氨酸含量维持较高的水平,而复水后叶绿素含量可以快速恢复,但膜脂过氧化程度维持较高水平;雄性黑杨在干旱胁迫时通过保持较高的细胞膜稳定性和稳定的抗氧化酶活性以提高抗旱性,同时降低叶绿素含量以减少水分损耗。表明在干旱条件下,雌性黑杨继续维持一定的光合和生长能力,但抗旱能力较低,而雄性黑杨相应具有较强的干旱耐受能力。此外,雄性黑杨无性系在应对干旱胁迫时表现出较为一致的生理响应,而雌性黑杨在无性系间存在较大差异。【结论】雄性黑杨无性系整体上具有较高且稳定的抗旱能力,而雌性黑杨抗旱能力相对较弱,且无性系间差异较大,但复水后的干旱间歇期可以较快恢复至正常的叶片生理状态。

关键词: 干旱胁迫, 南方型黑杨, 性别差异, 抗旱策略, 抗氧化酶系统

Abstract:

【Objective】 Climate change-induced drought restricts the distribution, growth and productivity of southern poplar plantations in the south of China. To explore adaptability to drought and provide reference for future sex-based poplar clone selection, we studied physiological responses to short-term intermittent soil drought between different sexes. 【Method】 Based on pot experiments by setting short-term intermittent soil water deficiency, four female and three male clones of southern-type poplar were used to compare sex-specific changes in physiological traits, including leaf plasma membrane stability, osmotic regulation, antioxidant system, and photosynthetic pigments. 【Result】 Short-term drought induced increases in electrical conductivity of leaves, malondialdehyde (MDA) and proline (Pro) contents, and antioxidant enzyme activities, but decreases in leaf chlorophyll content, for both male and female poplar clones, which indicated damage to leaf membranes and photosynthesis system with membrane lipid peroxidation. After drought relief by rewatering, these physiological traits all returned to substantially the same level as the (normal watering/non-drought) control treatment. Differences existed in the level and pattern of physiological responses to short-term soil drought between male and female clones. For female clones, the activity of antioxidant enzymes in leaves fluctuated greatly, and the contents of chlorophyll and proline remained at a high level under draught treatment; after drought relief by rewatering, chlorophyll content recovered rapidly, while membrane lipid peroxidation remained high. For male clones exposed to short-term drought, however, we detected stable POD activity and reduced chlorophyll content; this indicated higher drought resistance. Male clones, by maintaining membrane stability and reducing water loss. Therefore, female poplar clones showed relatively lower drought tolerance, but maintained a certain level of photosynthesis and growth, while male clones presented relatively higher drought tolerance. In addition, during short-term soil drought, three male clones showed relatively consistent physiological responses, while male clones had greater inter-clone variation. 【Conclusion】 Male poplar clones had relatively high and stable drought resistance, while female clones had generally weak but varied inter-clone drought resistance. However, female clones displayed rapid recovery of leaf physiological conditions after rewatering between short-term drought.

Key words: drought stress, southern-type poplar, sexual difference, drought resistance strategy, antioxidant enzyme system

中图分类号:

S722

马坛,田野,王书军,等. 不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应[J]. 南京林业大学学报（自然科学版）, 2024, 48(3): 172-180.

MA Tan, TIAN Ye, WANG Shujun, LI Wenhao, DUAN Qiying, ZHANG Qingyuan. Sex-specific leaf physiological responses of southern-type poplar to short-term intermittent soil drought[J].Journal of Nanjing Forestry University (Natural Science Edition), 2024, 48(3): 172-180.DOI: 10.12302/j.issn.1000-2006.202204059.

图/表 2

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不同性别南方型黑杨无性系叶片对土壤短期间歇性干旱的生理响应

Sex-specific leaf physiological responses of southern-type poplar to short-term intermittent soil drought

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