不同抗性松树家系中松材线虫致病力和繁殖力比较

doi:10.12302/j.issn.1000-2006.202302017

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南京林业大学学报（自然科学版） ›› 2025, Vol. 49 ›› Issue (1): 21-27.doi: 10.12302/j.issn.1000-2006.202302017

所属专题：专题报道：松材线虫病绿色防控研究

• 专题报道：松材线虫病绿色防控研究（执行主编叶建仁骆有庆） • 上一篇下一篇

不同抗性松树家系中松材线虫致病力和繁殖力比较

王俊伟¹^,²(), 胡龙娇³, 吴小芹¹^,^*()

1.南京林业大学林草学院,南方现代林业协同创新中心,江苏省有害生物入侵预防与控制重点实验室,江苏南京 210007
2.山东省烟台市昆嵛山林场,山东烟台 264112
3.江苏省中国科学院植物研究所,江苏南京 210014

收稿日期:2023-02-10 修回日期:2024-06-21 出版日期:2025-01-30 发布日期:2025-01-21
通讯作者: * 吴小芹(xqwu@njfu.edu.cn),教授。
作者简介:
王俊伟(ytwangjunwei@163.com)。
基金资助:
国家重点研发计划(2021YFD1400903);江苏高校优势学科建设工程资助项目(PAPD)

Comparison of pathogenicity and reproduction of Bursaphelenchus xylophilus in pine families with different disease resistance

WANG Junwei¹^,²(), HU Longjiao³, WU Xiaoqin¹^,^*()

1. Co-Innovation Center for Sustainable Forestry in Southern China, Jiangsu Key Laboratory for Prevention and Management of Invasive Species, College of Forestry and Grassland, Nanjing Forestry University, Nanjing 210037, China
2. Kunyushan Forest Farm of Yantai City of Shandong Province, Yantai 264112, China
3. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China

Received:2023-02-10 Revised:2024-06-21 Online:2025-01-30 Published:2025-01-21

摘要/Abstract

摘要：

【目的】探究在自然条件下松材线虫(Bursaphelenchus xylophilus)与不同抗病力松树家系互作后,是否对松材线虫的致病力和繁殖力产生影响,并分析致病力差异明显的松材线虫虫株与松树互作后其细胞色素 P450(Cytochrome P450)家族相关基因表达是否发生变化。【方法】对前期引自日本的抗松材线虫病赤松(Pinus densiflora)和黑松(P. thunbergii)基因库松树进行田间发病状况调查,发现不同松树家系的抗病能力存在显著差异。对松树基因库中各家系枯死木取样,经松材线虫自动化分子检测后,将确定为松材线虫致死的5个赤松家系和5个黑松家系样木进行线虫分离,采用单异活体培养共获得10个松材线虫虫株,检测这些虫株的致病力和繁殖力,并收集其中4个致病力差异明显的松材线虫虫株,采用RT-qPCR技术检测其与黑松互作后的2个细胞色素P450相关基因相对表达量差异。【结果】同一地块同一树种中抗病能力越强的家系,获得的松材线虫虫株在单异活体培养下繁殖量越低,相反抗病能力越弱的家系,获得的松材线虫繁殖量越高;将各虫株接种2年生黑松发现,5个黑松家系病死木中获得的线虫虫株致病力测定结果与松树家系的抗性强弱呈正相关,其中抗病能力最强的家系34病死木中获得的松材线虫虫株JYK-34的致病力最强。而赤松家系的抗性强弱与松材线虫的致病力并不呈正相关,其中来自抗病能力较强的家系18病死木分离的松材线虫虫株JYK-18的致病力却较弱;与对照线虫相比,致病力差异明显的4个虫株与2年生黑松苗互作30 d后,它们的2个细胞色素P450相关基因BxCYP33C4和BxCYP33C9的相对表达量增长倍数与虫株的致病力强弱呈正相关,各虫株依次为JYK-34>JYK-17>JYK-18>JYK-31。【结论】松材线虫繁殖力的分化在赤松和黑松两个树种不同抗病家系中规律相同;抗性松树基因库中同一地块不同抗病力松树分离的松材线虫的致病力并非单一类型,存在致病力差异的虫株;松材线虫细胞色素P450相关基因BxCYP33C4和BxCYP33C9不仅参与松材线虫与松树的互作过程,还是松材线虫重要的致病相关基因。

关键词: 赤松, 黑松, 松材线虫, 致病力, 繁殖力, 细胞色素P450相关基因

Abstract:

【Objective】 To explore whether the interaction of pine families with varying levels of disease resistance in the same plot with pine wood nematode (Bursaphelenchus xylophilus) under natural conditions affects the pathogenicity and reproduction of B. xylophilus, and to determine if the expression level of cytochrome P450 i.e. CYP family-related genes of B. xylophilus strains with noticeable differences in pathogenicity different after interaction with pine trees, the following study was conducted.【Method】This study monitored the gene pools of several families of Pinus densiflora and P. thunbergii, which were introduced from Japan previously in the lab. Field monitoring was conducted over several years, revealing significant differences in disease resistance among different pine families. Dead wood samples from each family in the pine tree gene pool were collected, and automated molecular detection was used to identify B. xylophilus. Nematode samples from five P. densiflora families and five P. thunbergii families, confirmed to have died from B. xylophilus, were isolated. A total of 10 B. xylophilus strains were obtained through monoheterotic in vivo culture, and their pathogenicity and reproduction were tested. The relative expression of two cytochrome P450 genes in four B. xylophilus strains with significantly different pathogenicity was detected by RT-qPCR. 【Result】The results indicated that in the same tree species within the same plot, the stronger the disease resistance of the pine families, the lower the reproduction rate of B. xylophilus strains obtained under monoheterotic living culture. Conversely, weaker disease resistance in pine families corresponded with higher reproduction rates of B. xylophilus strains. Inoculating each strain with 2-year-old P. thunbergii revealed that the pathogenicity test results of nematode strains obtained from diseased and dead trees of five P. thunbergii families were positively correlated with the resistance of pine families. The B. xylophilus strain JYK-34, isolated from the most resistant family (34), showed the highest pathogenicity. However, for P. densiflora families, there was no positive correlation between resistance and the pathogenicity of B. xylophilus. The B. xylophilus JYK-18, isolated from a highly resistant family (18), exhibited weak pathogenicity. Compared with the control B. xylophilus, the increased expression of two cytochrome P450 genes, BxCYP33C4 and BxCYP33C9, was positively correlated with the pathogenicity of the four B. xylophilus strains, namely JYK-34>JYK-17>JYK-18>JYK-31, after interacting with the P. thunbergii seedlings for 30 days. 【Conclusion】The differentiation in the reproduction of B. xylophilus was consistent among different disease-resistant families of P. densiflora and P. thunbergii. The pathogenicity of B. xylophilus isolated from pine trees with different resistance levels within the same plot in the resistant pine tree gene pool was not uniform, with different strains exhibiting varying pathogenicity. The cytochrome P450 genes BxCYP33C4 and BxCYP33C9 in B. xylophilus are involved in the interaction between B. xylophilus and pines and are also important genes related to the pathogenicity of B. xylophilus.

Key words: Pinus densiflora, P. thunbergii, Bursaphelenchus xylophilus, pathogenicity, reproduction, cytochrome P450 (CYP)

中图分类号:

S763

王俊伟,胡龙娇,吴小芹. 不同抗性松树家系中松材线虫致病力和繁殖力比较[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 21-27.

WANG Junwei, HU Longjiao, WU Xiaoqin. Comparison of pathogenicity and reproduction of Bursaphelenchus xylophilus in pine families with different disease resistance[J].Journal of Nanjing Forestry University (Natural Science Edition), 2025, 49(1): 21-27.DOI: 10.12302/j.issn.1000-2006.202302017.

图/表 5

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引物名称 name	序列(5'-3') sequence
qC9F1	GCGGTTTGCCATGAGACT
qC9R1	AAACGGGTGGGATCGAAT
qC4F1	AAGATCGACCGCCAGATG
qC4R1	CACCTCCAGCTGCATCCT
Actin F	GCAACACGGAGTTCGTTGTAGA
Actin R	GTATCGTCACCAACTGGGATGA

寄主 host	抗性家系编号 families No.	自然感病率/% natural incidence rate	抗病程度 disease resistance degree of host	分离的线虫虫株编号 B. xylophilus strains obtained No.
赤松 Pinus densiflora	18	0.8	高抗	JYK-18
	24	1.2	高抗	JYK-24
	17	2.3	中抗	JYK-17
	23	11.3	低抗	JYK-23
	普通赤松	14.8	低抗	PTCS
黑松 P. thunbergii	34	0.8	高抗	JYK-34
	32	1.3	高抗	JYK-32
	36	1.6	高抗	JYK-36
	31	2.2	中抗	JYK-31
	普通黑松	6.1	低抗	PTHS

虫株编号 strains No.	14 d		18 d		22 d		26 d		黑松发病至死亡的平均历期/d average duration from onset to death
虫株编号 strains No.	感病指数 disease severity index	感病率/% infection rate	感病指数 disease severity index	感病率% infection rate	感病指数 disease severity index	感病率/% infection rate	感病指数 disease severity index	感病率/% infection rate	黑松发病至死亡的平均历期/d average duration from onset to death
JYK-18	18.8	75.0	18.8	75.0	40.6	87.5	53.1	87.5	24
JYK-24	25.0	75.0	37.5	87.5	53.1	87.5	75.0	100.0	20
JYK-17	37.5	100.0	43.8	100.0	78.1	100.0	84.4	100.0	22
JYK-23	15.6	62.5	31.3	75.0	50.0	100.0	59.4	100.0	26
PTCS	15.6	62.5	34.4	87.5	68.8	100.0	75.0	100.0	20
JYK-34	37.5	100.0	56.3	100.0	75.0	100.0	87.5	100.0	20
JYK-32	25.0	75.0	50.0	87.5	65.6	100.0	84.4	100.0	20
JYK-36	34.4	100.0	40.6	100.0	59.4	100.0	75.0	100.0	22
JYK-31	6.3	25.0	12.5	37.5	15.6	37.5	34.4	75.0	30
PTHS	18.8	75.0	31.3	75.0	59.4	87.5	65.6	87.5	20

不同抗性松树家系中松材线虫致病力和繁殖力比较

Comparison of pathogenicity and reproduction of Bursaphelenchus xylophilus in pine families with different disease resistance

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[1]	陈友梅, 夏馨蕊, 叶建仁, 朱丽华. 马尾松胚性愈伤组织对松材线虫的抗性评价[J]. 南京林业大学学报（自然科学版）, 2025, 49(1): 37-45.
[2]	丁晓磊, 张悦, 林司曦, 叶建仁. 基于高通量测序技术的松材线虫研究进展[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 1-7.
[3]	韦鹏飞, 理永霞, 冯宇倩, 刘振凯, 张星耀. 松材线虫性别决定基因Bx-sex-1表达特征和生物学功能分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 15-20.
[4]	汪青桐, 丁晓磊, 叶建仁, 史秀峰. 基于SNP分子标记的华东地区松材线虫种群遗传分化研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 21-28.
[5]	王立超, 苏胜荣, 陈凤毛, 董晓燕, 田成连, 王洋. 黄山马尾松林天牛及携带线虫种类初步调查[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 29-35.
[6]	王磊, 叶建仁, 史丽娜. 利用腐生线虫加速替代疫木中松材线虫种群数量研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 36-44.
[7]	江奕, 陈凤毛. 喜旱莲子草与乌蔹莓等植物提取物对松材线虫的毒杀活性研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 45-52.
[8]	吴佳雯, 尹艳楠, 谈家金, 郝德君. 蜡样芽孢杆菌NJSZ-13菌株诱导马尾松抗松材线虫病研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 53-58.
[9]	张瑞芝, 姜生伟, 吴昊, 陈俏丽, 李丹蕾, 王峰. 松材线虫Bx-HSF-1转录激活活性研究[J]. 南京林业大学学报（自然科学版）, 2022, 46(4): 8-14.
[10]	高景斌, 徐六一, 叶建仁. 马尾松松材线虫病抗性无性系的筛选和遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 109-118.
[11]	许嘉麟, 谈家金, 郝德君. 蜡样芽孢杆菌NJSZ-13菌株对松材线虫产卵和繁殖的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(5): 209-214.
[12]	蒙海勤, 叶建仁, 王旻嘉, 曹伊扬. 木腐真菌对松材线虫病疫木处理初探[J]. 南京林业大学学报（自然科学版）, 2021, 45(4): 183-189.
[13]	尹艳楠, 谈家金, 李梦伟, 许嘉麟, 郝德君. 蜡样芽孢杆菌NJSZ-13菌株防治松材线虫病研究[J]. 南京林业大学学报（自然科学版）, 2021, 45(3): 152-158.
[14]	王洋, 陈军, 陈凤毛, 周权, 周立峰, 孙守慧. 松墨天牛取食期间传播松材线虫的特性[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 1-10.
[15]	尚征, 周立峰, 冯玥瑶, 刘费樱, 陈凤毛. 拟松材线虫个体发育研究[J]. 南京林业大学学报（自然科学版）, 2019, 43(6): 11-17.