南京林业大学学报(自然科学版) ›› 1990, Vol. 33 ›› Issue (03): 47-52.doi: 10.3969/j.jssn.1000-2006.1990.03.010

• 研究论文 • 上一篇    下一篇

褐剌蛾质型多角体病毒形态特征与生物活性测定

夏春胜;周性恒   

  1. 南京林业大学;南京林业大学
  • 出版日期:1990-09-18 发布日期:1990-06-18

A STUDY ON THE MORPHOLOGICAL CHARACTER AND BIOLOGICAL ACTIVITY OF THOSEA POSTORNATA CYTOPLASMIC POLYHEDROSIS VIRUS

Xia Chunsheng Zhou Xingheng   

  1. Nanj ing Forestry University
  • Online:1990-09-18 Published:1990-06-18

摘要: <正>本文描述了褐剌蛾质型多角体病毒的多角体和病毒粒子的形态特征,并测定了该病毒的生物活力。贮于4℃冰箱中1年的多角体悬液对3龄幼虫的LC_(50)为7.04×10~4CPB/ml;1×10°CPB/ml的多角体悬液对3龄、5龄幼虫的LT_(50)分别为9.6天和10.5天,即LT_(50)随龄期的加大而延长;新鲜多角体悬液(浓度为1×10°CPB/ml)涂于桑叶喂饲一龄、二龄和三龄家蚕,1天以后换正常桑叶(无毒)饲育,连续22天观察记录,无异常症状,也不致病。

Abstract: This paper deals with the morphological character and bioassay result of cytoplasmic polyhcdrosis virus of Thosea postornata. The early third instar larvae were fed with different concentration of the virus kept at 4℃ for 1 year, the LC50 value was 7.04×104 CPB/ml. The LT50 value for the virus (106 CPB / ml) to the third and the fifth instar larvae were 9.6 and 10.5 days respectively. In another word, the LT50 value increased with the increased instar. Silkworms were fed wity fresh Tp-CPV (10s CPB/ml) for 22 days, there were no death and abnormal symptoms.