不同蓝莓品种根际phoD基因相关土壤解磷细菌群落结构分析

doi:10.12302/j.issn.1000-2006.202007050

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (2): 95-102.doi: 10.12302/j.issn.1000-2006.202007050

不同蓝莓品种根际phoD基因相关土壤解磷细菌群落结构分析

吴叶娇¹(), 高源¹, 曹成亮¹, 蒋瑀霁³, 闾连飞², 吴文龙², 蒋继宏¹, 朱泓²^,^*(), 李荣鹏¹^,^*()

1.江苏师范大学生命科学学院,江苏省药用植物生物技术重点实验室,江苏徐州 221116
2.江苏省中国科学院植物研究所,江苏南京 210018
3.土壤与农业可持续发展国家重点实验室,中国科学院南京土壤所,江苏南京 210008

收稿日期:2020-07-05 接受日期:2021-04-29 出版日期:2022-03-30 发布日期:2022-04-08
通讯作者: 朱泓,李荣鹏
基金资助:
江苏省研究生科研与实践创新计划(KYCX20-2320);江苏省科技计划项目(BE2019399)

Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

WU Yejiao¹(), GAO Yuan¹, CAO Chengliang¹, JIANG Yuji³, LYU Lianfei², WU Wenlong², JIANG Jihong¹, ZHU Hong²^,^*(), LI Rongpeng¹^,^*()

1. Key Laboratory of Biotechnology for Medicinal Plant of Jiangsu Province, School of Life Science, Jiangsu Normal University, Xuzhou 221116, China
2. Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210018, China
3. State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China

Received:2020-07-05 Accepted:2021-04-29 Online:2022-03-30 Published:2022-04-08
Contact: ZHU Hong,LI Rongpeng

摘要/Abstract

摘要：

【目的】研究不同品种兔眼蓝莓(Vaccinium ashei Reade,简称RB)对phoD(碱性磷酸酶基因)相关土壤解磷细菌群落组成的影响,阐明解磷细菌对土壤磷素的转化以及植物生长的意义。【方法】通过对12个兔眼蓝莓品种根际土壤中phoD进行高通量测序,分析phoD基因相关土壤解磷细菌群落的多样性及组成,解析根际解磷细菌与蓝莓品种以及土壤理化性质之间的相互作用关系。【结果】在12个兔眼蓝莓品种中,蓝莓‘森土里昂’(‘Centurion’, RB4)品种具有最高的群落多样性;蓝莓根际phoD基因相关土壤解磷细菌群落核心细菌群由α-变形菌纲根瘤菌目、红螺菌目、β-变形菌纲伯克氏菌目、γ-变形菌纲黄单胞菌目、海洋螺菌目、假单胞菌目和放线菌门链霉菌目组成;蓝莓根际phoD基因相关土壤解磷细菌群落可以分为RBⅠ、RBⅡ两组,其中RBⅠ组的α-变形菌纲细菌相对丰度显著高于RBⅡ组;土壤总磷(TP)和phoD基因相关土壤解磷细菌群落组成是速效磷(AP)的主要影响因素。【结论】蓝莓不同品种和土壤性质对根际phoD基因相关解磷细菌群落丰度有显著影响,而群落多样性则与对照无显著差异,这可为进一步阐明酸性土壤条件下植物和根际解磷细菌之间的共生关系提供有效的数据支持。

关键词: 蓝莓, 根际, 土壤解磷细菌, 碱性磷酸酶基因, 速效磷

Abstract:

【Objective】The objective was to study the effects of different rabbiteye blueberry (Vaccinium ashei Reade, RB) cultivars on the composition of phoD soil phosphate solubilizing bacteria. 【Method】 Using the high-throughput sequencing of phoD (encoding an alkaline phosphatase gene) in rhizosphere soils of 12 rabbiteye blueberry cultivars, the diversity and composition of soil phoD phosphate-solubilizing bacterial communities were analyzed, and interactions between rhizosphere phosphate solubilizing bacteria, blueberry cultivars, and soil properties were analyzed. 【Result】Among the 12 rabbiteye blueberry cultivars, ‘Centurion’ (RB4) showed the highest community diversity; the core rhizosphere phoD bacterial community comprised the α-proteobacteria rhizobiales and rhodospirillales, the β-proteobacteria burkholderiales, the γ-proteobacteria xanthomonadales, oceanospirillales, pseudomonadales, and the actinobacteria streptomycetales. The phoD bacterial communities in the blueberry rhizosphere can be divided into two blueberry groups, RBⅠ and RBⅡ, and the relative abundance of α-proteobacteria in the RBⅠ group was significantly higher. The soil total phosphorus (TP) and phoD bacterial community composition were the main influencing factors of AP. 【Conclusion】Different blueberry cultivars and soil properties had significant effects on the abundance of rhizosphere phoD phosphate-solubilizing bacteria, however, the community diversity did not differ significantly from that of the control. These results provide an effective data support for further elucidation of the symbiotic relationship between plants and phosphate-solubilizing bacteria in the rhizosphere under acidic soil conditions.

Key words: blueberry, rhizosphere, soil phosphate solubilizing bacteria, alkaline phosphatase gene (phoD), available P

中图分类号:

S663

吴叶娇,高源,曹成亮,等. 不同蓝莓品种根际phoD基因相关土壤解磷细菌群落结构分析[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 95-102.

WU Yejiao, GAO Yuan, CAO Chengliang, JIANG Yuji, LYU Lianfei, WU Wenlong, JIANG Jihong, ZHU Hong, LI Rongpeng. Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(2): 95-102.DOI: 10.12302/j.issn.1000-2006.202007050.

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不同蓝莓品种根际phoD基因相关土壤解磷细菌群落结构分析

Community structure of phoD phosphate solubilizing bacteria in rhizosphere soil of different blueberry cultivars

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[1]	宋泽君, 李培培, 袁斓方, 郭小兰, 王德炉. 土壤含水率对蓝莓叶片生理及果实品质的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 147-156.
[2]	郭丽丽, 张晨洁, 王菲, 沈佳佳, 张凯月, 何丽霞, 郭琪, 侯小改. 牡丹野生种根际土壤细菌群落特征分析[J]. 南京林业大学学报（自然科学版）, 2023, 47(3): 45-55.
[3]	王阳, 王伟, 姜静, 顾宸瑞, 杨蕴力. 转基因小黑杨根际土壤微生物群落特征研究[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 199-208.
[4]	陈佳, 缑晶毅, 赵祺, 韩庆庆, 李慧萍, 姚丹, 张金林. 梭梭根际根瘤菌对紫花苜蓿生长及耐盐性的影响[J]. 南京林业大学学报（自然科学版）, 2021, 45(6): 99-110.
[5]	王爱斌, 宋慧芳, 张流洋, 张明, 杨诗雯, 张凌云. 生物肥和菌肥对蓝莓苗生长及土壤养分的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(6): 63-70.
[6]	周思婕, 王平, 张敏, 陈舒展, 许雯, 朱丽婷, 何销勤, 龚书锐. 大气酸沉降对马尾松幼苗根系生理特性的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(4): 111-118.
[7]	王小敏, 吴文龙, 闾连飞, 张春红, 杨海燕, 黄正金, 赵慧芳, 李维林. 蓝莓新品种‘寨选4号’[J]. 南京林业大学学报（自然科学版）, 2020, 44(3): 225-226.
[8]	杨海燕, 吴文龙, 闾连飞, 张春红, 黄正金, 王小敏, 赵慧芳, 李维林. 蓝莓新品种‘寨选7号’[J]. 南京林业大学学报（自然科学版）, 2020, 44(3): 227-228.
[9]	王邵军. “植物-土壤”相互反馈的关键生态学问题:格局、过程与机制[J]. 南京林业大学学报（自然科学版）, 2020, 44(2): 1-9.
[10]	夏晓雨, 王凤娟, 符群, 张娜, 郭庆启. 蓝莓果汁饮料原花青素的热稳定性及降解动力学模型[J]. 南京林业大学学报（自然科学版）, 2019, 43(5): 89-95.
[11]	姚蓓,赵慧芳,吴文龙,李维林. 蓝莓果实多酚提取物的抗炎活性研究[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 152-156.
[12]	杨海燕,董珊珊,闾连飞,黄正金,吴文龙,李维林. 三个高丛蓝莓品种在南京地区的田间生长表现[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 157-162.
[13]	赵慧芳,闾连飞,姚蓓,吴文龙,李维林. 蓝莓‘寨选’品系在南京地区的生长与结实表现[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 163-168.
[14]	董珊珊,李宁冉,杨海燕,吴文龙,闾连飞,李维林. 蓝莓根系对土壤锰胁迫的生理响应[J]. 南京林业大学学报（自然科学版）, 2019, 43(03): 169-174.
[15]	叶钰倩,赵家豪,刘畅,关庆伟. 间伐对马尾松人工林根际土壤氮含量及酶活性的影响[J]. 南京林业大学学报（自然科学版）, 2018, 42(03): 193-198.