4种基因表达量和光合参数差异对白桦无性系幼苗生长的影响

doi:10.12302/j.issn.1000-2006.202103037

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南京林业大学学报（自然科学版） ›› 2022, Vol. 46 ›› Issue (2): 88-94.doi: 10.12302/j.issn.1000-2006.202103037

4种基因表达量和光合参数差异对白桦无性系幼苗生长的影响

纳晓莹(), 刘刚, 刘桂丰, 王秀伟()

东北林业大学森林生态系统可持续经营教育部重点实验室,东北林业大学林学院, 黑龙江哈尔滨 150040

收稿日期:2021-03-22 接受日期:2021-08-20 出版日期:2022-03-30 发布日期:2022-04-08
通讯作者: 王秀伟
基金资助:
国家自然科学基金项目(31670476);黑龙江省应用技术研究与开发计划项目(GA20B401);中央高校基本科研业务费专项项目(2572019BA15);中央高校基本科研业务费专项项目(2572019CP09)

Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones

NA Xiaoying(), LIU Gang, LIU Guifeng, WANG Xiuwei()

Key Laboratory of Sustainable Forest Ecosystem Management, Ministry of Education, College of Forestry, Northeast Forestry University, Harbin 150040, China

Received:2021-03-22 Accepted:2021-08-20 Online:2022-03-30 Published:2022-04-08
Contact: WANG Xiuwei

摘要/Abstract

摘要：

【目的】分析1年生白桦(Betula platyphylla)无性系幼苗RBCS1A、RBCS3B、GO、RCA基因表达量和光合速率、蒸腾速率、气孔导度、瞬时水分利用效率、胞间CO₂浓度、羧化效率与苗高、地径的相关性,探索基因表达量和光合参数对白桦无性系幼苗生长初期的影响。【方法】在白桦双列杂交子代林中选择6株成熟的树木进行组织培养,随后将其扩繁为6个无性系,对1年生白桦无性系幼苗的生长量与光合参数和4个与光合作用相关的基因表达量进行测定分析。【结果】6种不同基因型白桦无性系幼苗的生长量与光合参数及基因表达量间差异显著(P<0.05);光合参数间表现出了密切的相关性,其中净光合速率与瞬时水分利用效率、蒸腾速率、气孔导度、羧化效率显著正相关(P<0.05)。RBCS1A的表达量与RBCS3B显著正相关,RBCS1A的表达量与RCA显著正相关,GO基因的表达量与RCA显著负相关(P<0.05)。基因表达量与植物的蒸腾速率、净光合速率、瞬时水分利用效率及气孔导度间均表现出了显著相关性(P<0.05),但基因表达量和光合参数与白桦幼苗的苗高、地径相关性未达到显著水平(P>0.05)。【结论】1年生白桦不同无性系幼苗4种基因的表达量和6种光合参数间差异显著,并且表现出了密切的相关性,但与苗高、地径相关性不显著。说明在白桦幼苗生长的初期,4种基因的表达量和6种光合参数并不能够显著地影响白桦无性系幼苗的生长。

关键词: 白桦无性系, 幼苗, 生长量, 基因表达量, 光合参数

Abstract:

【Objective】 We analyzed the relationship among RBCS1A, RBCS3B, GO and RCA gene expression in one-year-old white birch clone seedlings and photosynthetic rate, transpiration rate, stomatal conductance, instantaneous water use efficiency, intercellular CO₂ concentration, carboxylation efficiency, seedling height, and ground diameter of seedlings. Moreover, the effects of gene expression and photosynthetic parameters on the initial growth of birch clone seedlings were preliminarily explored. 【Method】 Six mature trees in a diallel cross progeny forest of white birch were cultured and propagated as six clones, and growth, photosynthetic parameters and expression of four photosynthesis-related genes in one-year-old white birch clone seedlings were determined and analyzed. 【Result】 There were significant differences in growth, photosynthetic parameters, and relative gene expression in seedlings of six different genotypes of white birch clones (P < 0.05). A significant correlation between photosynthetic parameters was observed, and the net photosynthetic rate was significantly positively correlated with instantaneous water use efficiency, transpiration rates, stomatal conductance, and carboxylation efficiency (P < 0.05); the relative expression of RBCS1A was significantly positively correlated with RBCS3B. The relative expression of RBCS1A was significantly positively correlated with RCA, and the relative expression of the GO gene was significantly negatively correlated with RCA (P < 0.05). A significant correlation between the relative gene expression and the plants’ transpiration rate, net photosynthetic rate, instantaneous water use efficiency, and stomatal conductance was observed (P < 0.05); however, the difference between the relative gene expression and photosynthetic parameters was not significantly correlated with the plant height and ground diameter (P < 0.05). 【Conclusion】 The expression levels of four genes and six photosynthetic parameters of different one-year-old white birch clone seedlings were significantly different and showed strong correlations, however, the correlation between the seedling height and ground diameter was not significant. This showed that in the early stage of birch seedling growth, the expression of the four examined genes and six photosynthetic parameters did not significantly affect the growth.

Key words: Betula platyphylla Suk. clone, seedling, growth, gene expression, photosynthetic parameters

中图分类号:

S718
S722.3

纳晓莹,刘刚,刘桂丰,等. 4种基因表达量和光合参数差异对白桦无性系幼苗生长的影响[J]. 南京林业大学学报（自然科学版）, 2022, 46(2): 88-94.

NA Xiaoying, LIU Gang, LIU Guifeng, WANG Xiuwei. Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones[J].Journal of Nanjing Forestry University (Natural Science Edition), 2022, 46(2): 88-94.DOI: 10.12302/j.issn.1000-2006.202103037.

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引物名 primer name	引物序列 primer sequence	温度/℃ temperature	产物长度/bp product length
BpGO-F	GGAGATTACCAATGTCAGCGAGTAT	68.1	77
BpGO-R	ACGCATAGTAGTCATACACCATCTTG	68.1	77
BpRCA-F	TGTCCAAGAGCAAGAGAATGTGAA	68.2	91
BpRCA-R	AATAGCATCCTGGTTAGCATCTCC	68.1	91
BpRBCS1A-F	CCAGAAGACCAACAACGACATTAC	67.6	126
BpRBCS1A-R	GAACTAAGTGGTGGAAGGTAGGAA	67.5	126
BpRBCS3B-F	GCAAGCTCAACAATGACATTACCT	67.7	97
BpRBCS3B-R	GTCTCAAACTTCTTCAATCCAACTGG	67.9	97
BpUBC-F	GGTTTCTGGGTTACAGTAAAGTTTCAG	67.7	76
BpUBC-R	CTTGAAGCCTGCATGTGGTC	67.8	76
BpSAND-F	CACAGAAGAGCCTTACGAGTCAT	67.9	135
BpSAND-R	CCTCCAAGCAAAGATGTCATATCAAAC	68.1	135

4种基因表达量和光合参数差异对白桦无性系幼苗生长的影响

Effects of the four genes expressions and photosynthetic parameters on seedlings growth of different birch clones

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温度/℃ temperature	时间/s time	循环数 number of cycles
95	60	1
95	15	45
60	60	45
95	60	1
55	30	1
95	30	1