ISSR在油茶品种鉴别和遗传多样性分析中的应用

于小玉; 喻方圆; 刘建兵; 陈军勇

doi:10.3969/j.issn.1000-2006.2013.01.010

于小玉,喻方圆,刘建兵,陈军勇

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (01) : 61-66.

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (01) : 61-66. DOI: 10.3969/j.issn.1000-2006.2013.01.010

研究论文

ISSR在油茶品种鉴别和遗传多样性分析中的应用

于小玉¹,喻方圆^1*,刘建兵²,陈军勇³

作者信息 +

Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker

YU Xiaoyu¹, YU Fangyuan^1*, LIU Jianbing², CHEN Junyong³

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文章历史 +

摘要

分子标记技术是准确鉴别油茶品种的重要手段之一。以湖南、湖北两省主要油茶品种为试验材料,利用ISSR分子标记技术分析了66个油茶品种的遗传多样性,揭示了各品种间的亲缘关系并对其进行了有效鉴别。结果表明:从100条引物中筛选出6条引物对66个油茶品种的基因组DNA进行扩增,获得了121条可以统计的扩增产物,其中多态性条带数(NPB)为112条,多态位点百分率为92.56%(PPB),平均等位基因观察值为1.933 9,平均有效等位基因为1.650 9,基因多样性指数即平均期望杂合度为0.369 8,Shannon信息指数为 0.541 7,说明油茶品种间的遗传多样性水平较高。根据扩增图谱条带的有无、多少和不同引物提供的谱带类型组合对供试油茶品种进行了鉴别。

Abstract

Molecular marker is one of the important ways for precise identification of Camellia oleifera varieties. The genetic diversity of 66 varieties of C. oleifera which were from Hunan and Hubei provinces was investigated using ISSR marker to reveal the affinity of these varieties. The results showed that: 6 ISSR primers selected from 100 primers were employed to conduct PCR amplification on genome DNA of 66 varieties of cultivated C. oleifera. And a total of 121 countable bands were amplified. Among them, the number of polymorphic bands(NPB)were 112, and percentage of polymorphic bands(PPB)was 92.56%. The average value of observed number of alleles, average value of effective number of alleles, Nei’s gene diversity and Shannon’s information index were 1.933 9,1.650 9, 0.369 8 and 0.541 7 respectively. The above parameters indicated that the level of genetic diversity in different varieties of C. oleifera was high. Different varieties of C. oleifera could be identified according to whether the polymorphic bands exist or not, the number of polymorphic bands, and the type of polymorphic bands amplified by different primers.

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于小玉,喻方圆,刘建兵,陈军勇. ISSR在油茶品种鉴别和遗传多样性分析中的应用[J]. 南京林业大学学报（自然科学版）. 2013, 37(01): 61-66 https://doi.org/10.3969/j.issn.1000-2006.2013.01.010

YU Xiaoyu, YU Fangyuan, LIU Jianbing, CHEN Junyong. Identification and genetic diversity analysis of Camellia oleifera varieties using ISSR marker[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(01): 61-66 https://doi.org/10.3969/j.issn.1000-2006.2013.01.010

中图分类号： S722

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基金

收稿日期:2011-09-07 修回日期:2012-11-28
基金项目:江苏高校优势学科建设工程资助项目(PAPD)
第一作者:于小玉,硕士生。*通信作者:喻方圆,教授。E-mail: fyyu@njfu.com.cn。
引文格式:于小玉,喻方圆,刘建兵,等. ISSR在油茶品种鉴别和遗传多样性分析中的应用[J]. 南京林业大学学报:自然科学版,2013,37(1):61-66.