南京林业大学学报(自然科学版) ›› 2013, Vol. 37 ›› Issue (01): 67-70.doi: 10.3969/j.issn.1000-2006.2013.01.011

• 研究论文 • 上一篇    下一篇

麝香百合及东方百合品种核型分析

房 磊,姜 珊,席梦利*,施季森,傅 伟,杨 斌   

  1. 南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,江苏 南京 210037
  • 出版日期:2013-02-18 发布日期:2013-02-18
  • 基金资助:
    收稿日期:2011-09-02 修回日期:2012-02-28
    基金项目:国家自然科学基金项目(30972407); 江苏省农业科技自主创新资金项目(CX(12)2018); 江苏高校优势学科建设工程资助项目(PAPD)
    第一作者:房磊,硕士。*通信作者:席梦利,副教授。E-mail: ximenglinjfu@126.com。
    引文格式:房磊,姜珊,席梦利,等. 麝香百合及东方百合品种核型分析[J]. 南京林业大学学报:自然科学版,2013,37(1):67-70.

The karyotype analysis of longiflorum lily and oriental lily

FANG Lei, JIANG Shan, XI Mengli*, SHI Jisen, FU Wei, YANG Bin   

  1. Key Laboratory of Forest Genetics and Biotechnology Ministry of Education, Nanjing Forestry University, Nanjing 210037, China
  • Online:2013-02-18 Published:2013-02-18

摘要: 以麝香百合‘白狐狸’(‘White Fox’)和‘雷山’(‘Lei Shan’)、东方百合‘水晶布兰卡’(‘Crystal Blanca’)和‘康斯坦撒’(‘Constansa’)的试管苗根尖为研究材料,用环己酰亚胺预处理,然后卡诺氏固定液固定,纤维素酶和果胶酶解离后采用涂片法制片,对4种观赏百合进行核型分析。结果表明,‘白狐狸’核型为3A,‘雷山’、‘水晶布兰卡’和‘康斯坦撒’的核型均为3B。核型公式分别为:‘白狐狸’2n=2x=24=4m+4st(2SAT)+16t,‘雷山’2n=2x=24=2m+2sm+10st(2SAT)+10t,‘水晶布兰卡’2n=2x=24=4m+10st+10t,‘康斯坦撒’2n=2x=24=4m(1SAT)+10st+10t。‘白狐狸’、‘雷山’、‘水晶布兰卡’及‘康斯坦撒’的核不对称系数分别为83.11%、82.78%、79.75%、81.22%。

Abstract: Plantlet root tips of longiflorum hyhrids ‘White Fox’ and ‘Lei Shan’, oriental hybrids ‘Crystal Blanca’ and ‘Constansa’ were used as materials to analyze karyotype. The root tips were pretreated with cyclohexamide then fixed in Carnoy’s solution and cell wall was digested with cellulase and pectinase. Finally, spreading method was used for preparing slides. The karyotype analysis result indicated that the karyotype of ‘White Fox’ was 3A, that of others were 3B. The karyotype formula was 2n=2x=24=4m+4st(2SAT)+16t for ‘White Fox’, 2n=2x=24=2m+2sm+10st(2SAT)+10t for ‘Lei Shan’, 2n=2x=24=4m+10st+10t for ‘Crystal Blanca’, 2n=2x=24=4m(1SAT)+10st+10t for ‘Constansa’. Karyotype asymmetry coefficient were 83.11%, 82.78%, 79.75%, 81.22%, respectively.

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