不同菌株绿僵菌对粒肩天牛幼虫的致病力及林间防治效果

doi:10.3969/j.issn.1000-2006.2013.01.013

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (01): 78-82.doi: 10.3969/j.issn.1000-2006.2013.01.013

不同菌株绿僵菌对粒肩天牛幼虫的致病力及林间防治效果

陈德兰

武夷山市林业局,福建武夷山 354300

出版日期:2013-02-18 发布日期:2013-02-18
基金资助:
收稿日期:2012-02-10 修回日期:2012-05-15
基金项目:福建省科技厅重点科技资助项目(2004N013); 南平市科技局科技资助项目(N2005Z12)
第一作者:陈德兰,高级工程师。E-mail: wyscdl@163.com。
引文格式:陈德兰. 不同菌株绿僵菌对粒肩天牛幼虫的致病力及林间防治效果[J]. 南京林业大学学报:自然科学版,2013,37(1):78-82.

Control efficiency and pathogenicity in different strains of Metarhizium anisopliae on Apriona germari Hope larvae

CHEN Delan

Wuyishan City Forestry Bureau,Wuyishan 354300,China

Online:2013-02-18 Published:2013-02-18

摘要/Abstract

摘要： 粒肩天牛是福建省杨树林的一种主要害虫,此次研究应用7株金龟子绿僵菌菌株对该天牛幼虫进行生物测定,旨在筛选出感染该虫的高致病力菌株,为生物防治提供新的资源。结果表明:感染Ma1291-2和Ma1775两个绿僵菌菌株14 d后,天牛幼虫的校正死亡率分别为89.47%和88.92%; Ma1291-2和Ma1775两个绿僵菌株对天牛幼虫的致死中时间(LT₅₀)分别为5.62和5.82 d, 孢子致死中浓度(LC₅₀)分别为1.70×10⁴个/mL和6.16×10⁴个/mL。说明绿僵菌Ma1291-2和Ma1775两个菌株对该天牛幼虫的致病力强,不仅致死率高,且致死速度快,僵虫率高,应以浓度为1.5×10⁸个/mL的孢子悬浮液进行林间防治,效果良好。

Abstract: Apriona germari Hope is a main insect pest of poplar in Fujian province. In order to screen high virulence strains from Metarhizium anisopliae against A. germari larvae, bioassays from seven strains of M. anisopliae were carried out through dipping in spore suspension. The results showed that the cumulative corrected mortality of A. germari larvae inoculated with Ma1291-2 and Ma1775 strains reached to 89.47% and 88.92% after 14 d treatment. While LT₅₀ value of the two strains were 5.62 d and 5.82 d, and LC₅₀ value were 1.70×10⁴spore/mL and 6.16×10⁴spore/mL, respectively. It could be concluded that Ma1291-2 and Ma1775 had higher virulence than the other strains,with rapid growth and high sporulation. A separate field bioassay suggested that Ma1291-2 and Ma1775 could be used to control A. germari larvae, with 1.5×10⁸ spore/mL.

中图分类号:

陈德兰. 不同菌株绿僵菌对粒肩天牛幼虫的致病力及林间防治效果[J]. 南京林业大学学报（自然科学版）, 2013, 37(01): 78-82.

CHEN Delan. Control efficiency and pathogenicity in different strains of Metarhizium anisopliae on Apriona germari Hope larvae[J].Journal of Nanjing Forestry University (Natural Science Edition), 2013, 37(01): 78-82.DOI: 10.3969/j.issn.1000-2006.2013.01.013.

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不同菌株绿僵菌对粒肩天牛幼虫的致病力及林间防治效果

Control efficiency and pathogenicity in different strains of Metarhizium anisopliae on Apriona germari Hope larvae

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