通过室内饲养产卵期光肩星天牛成虫并采集其产卵分泌物,对光肩星天牛产卵分泌物进行组分分析。利用烘干法、Bradford法、蒽酮比色法、茚三酮试剂显色法、SDS-PAGE对其组分中水、蛋白质、可溶性糖、游离氨基酸的含量及蛋白质组分进行了测定。结果表明:光肩星天牛产卵分泌物组分中含水量为81.98%,蛋白质含量为4.14%,可溶性糖及游离氨基酸的含量依次为0.51%和0.06%(均为质量分数),蛋白质组分中主要蛋白的分子质量为107.1、86.7、62.2、47.5、39.0 ku,其中107.1～86.7 ku与47.5 ku为强谱带区。
Abstract
To analyze the composition of Anoplophora glabripennis oviposition secretion, A.glabripennis were kept indoors during the spawning period, and oviposition secretion of which was collected. The oven drying method, Bradford method, anthrone colorimetry, Ninhydrin reagent colorimetric method and SDS-PAGE were used to analyze and determinate the fractions of water, protein, soluble sugar and free amino acid content and protein fractions in oviposition secretion. The results showed that oviposition secretion components of A.glabripennis included water(81.98%), protein(4.14%), soluble sugar(0.51%)and free amino acid(0.06%). The molecular weight of main protein components were 107.1 ku,86.7 ku,62.2 ku,47.5 ku and 39.0 ku. The strong spectral bands were 107.1 ku to 86.7 ku and 47.5 ku.
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参考文献
[1] 李成德.森林昆虫学[M].北京:中国林业出版社,2004.
[2] 金凤,嵇保中,刘曙雯,等.天牛产卵分泌物的研究概述[J].金陵科技学院学报,2009,25(1):74-77. Jin F, Ji B Z, Liu S W, et al. A review on the ovipositing secretion of Apriona germari[J]. Journal of Jinling Institute of Technology, 2009,25(1):74-77.
[3] 王峰,骆有庆,田桂芳, 等.光肩星天牛成虫寄主选择中的“记忆效应”[J].中国森林病虫,2007, 26(4):11-14. Wang F, Luo Y Q, Tian G F, et al. Memory effect in host tree selection of Anoplophora glabripennis adult[J]. Forest Pest and Disease, 2007,26(4):11-14.
[4] 高瑞桐,Wang B D,Victor C M,等.光肩星天牛对白桦林的危害及药剂防治效果[J].林业科学,2009,45(2):163-166. Gao R T, Wang B D, Mastro V C, et al. Infestation of Betula platyphylla by Anoplophora glabripennis and its control using insecticides[J]. Scientia Silvae Sinicae, 2009,45(2):163-166.
[5] Peterson A T, Scachetti-Pereira R. Potential geographic distribution of Anoplophora glabripennis(Coleoptera: Cerambycidae)in north America[J]. The American Midland Naturalist, 2004,151(1):170-178.
[6] Hajek A E, Lund J, Smith M T. Reduction in fitness of female Asian longhomed beetle(Anoplophora glabripennis)infected with Metarhizium anisopliae[J]. Journal of Invertebrate Pathology, 2008, 98(2):198-205.
[7] 曹传旺,高彩球.昆虫生化与分子生物学实验技术[M].哈尔滨:东北林业大学出版社,2009.
[8] 陈锍荃.生物化学实验方法和技术[M].北京:科学出版社,2003.
[9] 汪家政,范明.蛋白质技术手册[M].北京:科学出版社,2000.
[10] Levent G Ltekin, Boris A Korotyaev. Biology and distribution of Larinus sibirinus Gyllenhal(Coleoptera: Curculionidae, Lixinae)[J]. J Ent Res Soc, 2005, 7(3):47-52.
[11] Mudd A, Ferguson A W, Blight M M, et al. Extraction, isolation, and composition of oviposition-deterring secretion of cabbage seed weevil Ceutorhynchus assimilis[J]. Journal of Chemical Ecology, 1997, 23(9):2227-2240.
[12] 马晓乾,王茜,邓勋,等.光肩星天牛在糖槭上产卵部位的选择及刻槽产卵习性研究[J].安徽农业科学,2012,40(7):4078-4079. Ma X Q, Wang Q, Deng X, et al. Studies on oviposition site selection of Anoplophora glabripennis in Acer saccharum and its gnawing nidus oviposition habits[J]. Journal of Anhui Agri Sci, 2012,40(7):4078-4079.
[13] 金凤,嵇保中,刘曙雯,等.桑天牛刻槽产卵分泌物组成成分[J].南京林业大学学报:自然科学版,2008,32(2):83-86. Jin F, Ji B Z, Liu S W, et al. Study on ingredient of the oviposition secretion of Apriona germari(Hope)[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2008,32(2):83-86.
[14] Shawna P, Peter D G, Sandy S. Oviposition behaviour and response of Monochamus scutellatus (Coleoptera: Cerambycidae)to conspecific eggs and larvae[J].Agricultural and Forest Entomology, 2002, 4(3):217-222.
[15] Anbutsu H, Togashi K. Deterred oviposition response of Monochamus alternatus(Coleoptera: Cerambycidae)to oviposition scarsoccupied by eggs[J]. Agricultural and Forest Enotomology, 2000, 2(3):217-223.
基金
收稿日期:2012-03-20 修回日期:2012-10-15
基金项目:黑龙江省青年科学基金项目(QC2009C64)
第一作者:马晓乾,助理研究员。E-mail:369086324@qq.com。
引文格式:马晓乾,赵红盈,周琦,等. 光肩星天牛产卵分泌物的化学成分分析[J]. 南京林业大学学报:自然科学版,2013,37(1):83-86.
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