楸树自交及种内、种间杂交亲和性的细胞学观察

樊莉丽; 彭方仁; 王改萍; 郝明灼; 张雪英

doi:10.3969/j.issn.1000-2006.2013.04.001

樊莉丽, 彭方仁, 王改萍, 郝明灼, 张雪英

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (04) : 1-7.

PDF(2023 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(2023 KB)

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (04) : 1-7. DOI: 10.3969/j.issn.1000-2006.2013.04.001

研究论文

楸树自交及种内、种间杂交亲和性的细胞学观察

樊莉丽^1,2,彭方仁^1*,王改萍¹,郝明灼¹,张雪英¹

作者信息 +

Cytological observation of fertilization compatibility of Catalpa bungei after self, intraspecific cross and interspecific cross-pollination

FAN Lili^1,2,PENG Fangren^1*,WANG Gaiping¹,HAO Mingzhuo¹,ZHANG Xueying¹

Author information +

文章历史 +

摘要

运用荧光显微技术、整体透明法和石蜡切片法,对楸树自交及种内、种间杂交亲和性进行了显微观察。结果表明:无论自交或种内、种间杂交花粉均可在柱头萌发,但萌发时间和萌发率存在显著差异。花粉管在柱头分泌物中生长或穿透乳突细胞沿柱头中部维管束生长,之后进入花柱引导组织胞间隙。大量种内和种间杂交花粉管于授粉后12～24 h迅速生长至花柱基部; 48～72 h进入子房; 96～120 h前后发生双受精,肉眼可见子房发红膨大。自交授粉后约36%的花粉管在柱头和花柱上端终止生长; 42%的花粉管于授粉后72～96 h进入子房; 直到授粉后120 h花冠脱落为止,少数花粉管能够穿透胚囊,但未见双受精发生,子房无膨大。研究认为:楸树具有自交不亲和性,而种内和种间杂交是亲和的; 其不亲和反应发生的最终位置在子房,时间在合子形成前,为合子前晚期自交不亲和类型。

Abstract

By means of fluorescence microscopic technique, whole stain-clearing technique, and paraffin sectioning procedure, the fertilization compatibility of Catalpa bungei after self, intraspecific and interspecific cross-pollination were examined. It was founded that pollens could germinate whether self pollination or intraspecific and interspecific pollination. But both the germination time and germination rate was significantly different. The pollen tubes either grew in the secretions of stigmas or penetrated the papilla cells, and then went along the inner vascular bundle, finally grew into the styles along the intercellular space of transmitting tissues. Most pollen tubes grew quickly to the basement of the style at 12 to 24 hours after intraspecific and interspecific pollination, and entered the ovary after 1 or 2 days entered the ovary. Double fertilization was observed about 96 to120 hours after cross-pollination. At the same time we saw with naked eyes that the ovaries turned red and swelling. After self-pollination, 36% of pollen tubes ceased on the stigma or in the upper styles. 42% of pollen tubes could grow into the ovaries at 72 to 96 hours after self-pollination. Double fertilization could not be observed although part of pollen tubes penetrated the ovules. Pistils fell off at 120 hours after self-pollination without presenting any swelling of the ovary. It was clear that C. bungei exhibited self-incompatibility, intraspecific cross-compatibility, and interspecific cross-compatibility. Because the site of pollen tube termination was in the ovary, and the time was pre-zygotic, C. bungei was considered to be a pre-zygotic late-acting self-incompatibility type.

导出引用

樊莉丽, 彭方仁, 王改萍, 郝明灼, 张雪英. 楸树自交及种内、种间杂交亲和性的细胞学观察[J]. 南京林业大学学报（自然科学版）. 2013, 37(04): 1-7 https://doi.org/10.3969/j.issn.1000-2006.2013.04.001

FAN Lili, PENG Fangren, WANG Gaiping, HAO Mingzhuo, ZHANG Xueying. Cytological observation of fertilization compatibility of Catalpa bungei after self, intraspecific cross and interspecific cross-pollination[J]. Journal of Nanjing Forestry University (Natural Sciences Edition）. 2013, 37(04): 1-7 https://doi.org/10.3969/j.issn.1000-2006.2013.04.001

中图分类号： Q321

参考文献

[1] 郭从俭,钱士金,王连卿,等.楸树栽培[M].北京:中国林业出版社,1988.
[2] 郝明灼,彭方仁,王改萍,等.楸树人工杂交授粉试验及种实分析[J].南京林业大学学报:自然科学版,2008,32(5):131-134. Hao M Z,Peng F R,Wang G P,et al. Artificial pollination test and analysis of hybrid seed of Catalpa bungei[J].Journal of Nanjing Forestry University:Natural Sciences Edition,2008,32(5):131-134.
[3] 王苏珂,王军辉,张守攻,等.楸树不同交配组合种子发芽特性的研究[J].林业科学研究,2008,21(2):275-278. Wang S K,Wang J H,Zhang S G,et al.Study on seed germinating characteristics among different combination of Catalpa bungei[J]. Forest Research, 2008,21(2):275-278.
[4] 王小艳,赵鲲,赵牧峰,等.楸树杂交育种初报[J].河南林业科技,2008,28(2):16-18.
[5] 贾继文,王军辉,张金凤,等.梓树属花粉生活力的研究[J].西北植物学报,2009,29(5):867-873. Jia J W,Wang J H,Zhang J F, et al. Pollen vitality of Catalpa Scop.[J]. Acta Botanica Boreali-Occidentalia Sinica, 2009,29(5):867-873.
[6] 王改萍,杨红宁,倪果果,等.楸树等4 种梓属树种花粉离体培养条件的研究[J].植物资源与环境学报,2009,18(2):34-42. Wang G P,Yang H N,Ni G G,et al.Study on in vitro culture conditions of pollens from four tree species of Catalpa Scop.including Catalpa bungei[J]. Journal of Plant Resources and Environment, 2009,18(2):34-42.
[7] Lai Z,Cui H Y,Yang H J,et al.Molecular biology of self and non-self pollen recognition in flowering plants [J].Development and Reproductive Biology,1999,18(2):65-70.
[8] 陈晓流,束怀瑞,陈学森.核果类果树自交不亲和性研究进展[J].植物学通报,2004,21(6):755-764. Chen X L,Shu H R,Cheng X S.Studies on self-incompatibility in stone fruit trees[J]. Chinese Bulletin of Botany, 2004,21(6):755-764.
[9] 刘林德,张洪军,祝宁,等.刺五加花粉活力和柱头可授性的研究[J].植物研究,2001,21(3):375-379. Liu L D,Zhang H J,Zhu N,et al.Pollen viability and stigma receptivity of Eleutherococcus senticosus(Araliaceae)[J]. Bulletin of Botanical Research,2001,21(3):375-379.
[10] 杨弘远,周嫦.植物有性生殖实验研究四十年[M].武汉:武汉大学出版社,2001.
[11] 胡适宜.植物胚胎学实验方法(五):检查花粉在柱头上萌发和花粉管在花柱中生长的制片法[J].植物学通报,1994,11(1):58-60. Hu S Y.Method of preparation of slides used to examine the pollen germination on the stigma and pollen tube growth in the style[J]. Chinese Bulletin of Botany, 1994,11(1):58-60.
[12] 王彩云,高莉萍,鲁涤非,等.‘厚瓣金桂’桂花花芽形态分化的研究[J].园艺学报,2002,29(1):52-56. Wang C Y,Gao L P,Lu D F,et al.A Study on morphological differentiation of flower bud of Osmanthus fragrans ‘Houban Jingui’[J]. Acta Horticulturae Sinica,2002,29(1):52-56.
[13] 杨杰,于小艳,李玉花.植物亲和受精过程中花粉管的黏附和定向生长[J].植物生理学通讯,2004,40(6):659-665. Yang J,Yu X Y,Li Y H.Adhesion and directional growth of pollen tubes in the compatible pollination of plant[J]. Plant Physiology Communications, 2004,40(6):659-665.
[14] 张桂玲,王超,温四民.甘蓝自交不亲和性的快速测定[J].东北农业大学学报,2003,34(2):142-147. Zhang G L,Wang C,Wen S M.Identification of the self-incompatibility of cabbage by fluorescence microscopy[J]. Journal of Northeast Agricultural University, 2003,34(2):142-147.
[15] Krichevsky A,Kozlovsky S V,Tian G W,et al.How pollen tubes grow[J].Developmental Biology,2007,303:405-420.
[16] 孙颖,孙大业.花粉萌发和花粉管生长发育的信号转导[J].植物学报,2001,43(12):1211-1217. Sun Y,Sun D Y. Signal transduction in pollen germination and tube growth[J].Acta Botanica Sinica, 2001,43(12):1211-1217.
[17] 胡适宜,杨弘远.被子植物受精生物学[M].北京:科学出版社,2002.
[18] Barrett S C H. The Evolution,Maintenance,and Loss of Self-incompatibility Systems[M].Oxford:Oxford University Press,1988.
[19] Seavey S R,Bawa K S.Late-acting self-incompatibility in angiosperms[J].The Botanical Review,1986,52:195-219.
[20] Kenrick J,Kaul V,Williams E G.Self-incompatibility in Acacia retinodes:site of pollen-tube arrest is the nucellus[J].Planta,1986,169:245-250.
[21] Knight R,Rogers H H.Incompatibility in Theobroma cacao[J].Heredity,1955,9:69-77.
[22] Lipow S R,Wyatt R.Single gene control of postzygotic self-incompatibility in poke milkweed[J].Genetics,2000,154:893-907.
[23] Bittencourt N S,Semir J.Late-acting self-incompatibility and other breeding systems in Tabebuia(Bignoniaceae)[J].Plant Science,2005,166:493-506.
[24] Valtuena F J,Rodriguez-Riano T,Espinosa F, et al.Self-sterility in two Cytisus species(Leguminosae,Papilionoideae)due to early-acting inbreeding depression[J].American Journal of Botany,2010,97:123-135
[25] Gibbs P E,Bianchi M B.Does late-acting self-icompatibility(LSI)show family clustering two more species of Bignoniaceae with LSI:Dolichandra cynan-choides and Tabebuia nodosa[J].Annals of Botany,1999,84:449-457.
[26] 胡适宜.被子植物生殖生物学[M].北京:高等教育出版社,2005.
[27] Takayama S,Isogai A.Self-incompatibility in plants[J].Annual Review of Plant Biology,2005,56:467-489.
[28] 蓝兴国,于晓敏,李玉花.配子体自交不亲和信号转导的研究进展[J].遗传,2005,27(4):677-685. Lan X G,Yu X M,Li Y H. Progress in study on signal transduction of gametophytic self-incompatibility[J]. Hereditas, 2005,27(4):677-685.
[29] Savitsky H I.A method of determining self-fetility and self-sterility in sugar beets,based upon the stage of ovule development shortly after flowering[J].Suagr beet Technol,1950,1:198-201.
[30] 吕政恒.甜菜自交不亲和性识别机制的初步研究[D].哈尔滨:哈尔滨工业大学,2010. Lyu Z H. The primary study of self-incompatibility recognition mode in sugar beet[D].Harbin: Harbin Institute of Technology,2010.
[31] Larsen K.Self-incompatibility in Beta vulgaris L.I.Four gametophytic complementary S-loci in sugar beet[J].Hereditas,1977,85(2):227-248.

基金

收稿日期:2012-03-16 修回日期:2013-04-03
基金项目:“十二五”国家科技支撑计划(2012BAD21B03); 江苏省农业科技支撑项目(BE2010311)
第一作者:樊莉丽,博士生。*通信作者:彭方仁,教授,博士。E-mail: frpeng@ njfu. edu. cn。
引文格式:樊莉丽,彭方仁,王改萍,等. 楸树自交及种内、种间杂交亲和性的细胞学观察[J]. 南京林业大学学报:自然科学版,2013,37(4):1-7.