Abstract: In order to choose the best botanical insecticide for controlling Asian gypsy moth, Lymantria dispar asiatica, we compared toxicities and antifeedant effects of four botanical insecticides on the 3rd instar larvae, including 5% Rotenone, 1%resmethrin-matrine, 5% eucalyptole and 0.6% Oxymatrine-lactone. The results indicated that the four kinds of botanical insecticides all had the functions of stomach poison and contact toxicity, and the acute toxicity performance showed that the function of contact toxicity was more obvious than stomach poison. When botanical insecticides were diluted at the highest concentration, the stomach poison of 0.6% Oxymatrine-lactone had the most mortality, followed by 5% eucalyptole. Due to the LC₅₀ of stomach poison groups were all less than that of contact toxicity groups, the stomach poison had a more toxic. Moreover,the toxicities of 0.6% Oxymatrine-lactone and 5% eucalyptole were much higher than the other two botanical insecticides, and the toxicities of 0.6% Oxymatrine-lactone was the strongest. Comparing the weight of feedingstuff before and after feeding,we found that the antifeedant effects of Asian gypsy moth 3rd instar larvae performed well under the four kinds of botanical insecticides treatments, and the antifeedant rate under 5% eucalyptole diluted 5 000 times reached to 99.31%. It was deduced that 5% eucalyptole and 0.6% Oxymatrine-lactone could be popularized and applied as botanical insecticides to control Asian gypsy moth.