油茶带芽茎段及叶片离体培养再生体系的建立

袁德义; 范晓明; 谭晓风; 曾艳玲; 唐静; 杨亚

doi:10.3969/j.issn.1000-2006.2013.05.007

袁德义，范晓明，谭晓风，曾艳玲，唐静，杨亚

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 35-39.

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 35-39. DOI: 10.3969/j.issn.1000-2006.2013.05.007

研究论文

油茶带芽茎段及叶片离体培养再生体系的建立

袁德义，范晓明，谭晓风，曾艳玲，唐静，杨亚

作者信息 +

Culture in vitro and rapid propagation techniques of buds and leafs in Camellia oleifera

YUAN Deyi, FAN Xiaoming, TAN Xiaofeng, ZENG Yanling, TANG Jing, YANG Ya

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文章历史 +

摘要

以油茶优良无性系‘华鑫’的春季嫩梢带芽茎段和叶片为外植体，用不定芽作为材料研究了植物生长调节剂对不定芽的增殖和生根的影响,同时分析了不同基质对组培苗移栽成活率的影响。结果表明：（1）将带芽茎段接种在1/2MS +NAA（ 05 mg/L）+6-BA（10 mg/L）+ZT（05 mg/L）的培养基中腋芽诱导率最高可达745%；叶片接种在1/2MS+TDZ （10 mg/L）+IAA（01 mg/L）+6-BA（20 mg/L）培养基中培养7周后可产生不定芽。（2）不定芽切成小块或单芽后转到1/2MS+6-BA（10 mg/L）+NAA（005 mg/L）中生长良好，增殖系数为417。（3）试管苗经1 g/L的IBA处理5 s转入1/2MS空白培养基50 d后，生根率高达883%，平均根长372 cm，每株平均根数8条。（4）组培苗移栽到泥炭和蛭石体积比例为1∶1的混合基质中，成活率可达825%。

Abstract

The effects of plant growth regulators on the adventitious bud proliferation and rooting in Camellia oleifera were studied by using new shoot (with buds) and leaf as explants to establish a fast reproductive system. And the effects of different medium on survival rate of transplanted tissue culture seedlings were also investigated. The results showed that:(1) The highest frequency of shoot formation was obtained when explants (stem with buds) were cultured on 1/2MS+NAA(0.5 mg/L)+6-BA(1.0 mg/L)+ZT(0.5 mg/L). The inducement rate of valid buds reached to 745%. The regenerated shoot from leaf was obtained when cultured on 1/2MS+TDZ (1.0 mg/L)+IAA(0.1 mg/L)+6-BA(2.0 mg/L) for 7 weeks. (2) Regenerated shoot were cut as several cluster or single plantlet and transferred on 1/2MS+6-BA(1.0 mg/L)+NAA(0.05 mg/L) for shoot propagation and growth, which the multiplication coefficient was more than 417. (3) 883% of rooting rate, 372 cm of average root length, and 8 roots of average plantlet were observed when cultured in 1/2 MS for 50 days, after shoot was dipped in 1 g/L IBA for 5 second. (4) The rooted plantlets were acclimatized on mixture of peat and vermiculite(1∶1), which survival rate was 82.5%.

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袁德义，范晓明，谭晓风，曾艳玲，唐静，杨亚. 油茶带芽茎段及叶片离体培养再生体系的建立[J]. 南京林业大学学报（自然科学版）. 2013, 37(05): 35-39 https://doi.org/10.3969/j.issn.1000-2006.2013.05.007

YUAN Deyi, FAN Xiaoming, TAN Xiaofeng, ZENG Yanling, TANG Jing, YANG Ya. Culture in vitro and rapid propagation techniques of buds and leafs in Camellia oleifera[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(05): 35-39 https://doi.org/10.3969/j.issn.1000-2006.2013.05.007

中图分类号： S722

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基金

收稿日期：2012-08-20修回日期：2012-11-01 基金项目：国家林业科技推广项目“大果油茶高产新品种示范推广”（[2011] 21号）第一作者：袁德义,教授。 Email：yuandeyi@163.com。引文格式：袁德义，范晓明，谭晓风，等. 油茶带芽茎段及叶片离体培养再生体系的建立[J]. 南京林业大学学报：自然科学版，2013，37（5）：35-39.