南京林业大学学报(自然科学版) ›› 2013, Vol. 37 ›› Issue (05): 153-156.doi: 10.3969/j.issn.1000-2006.2013.05.029

• 研究简报 • 上一篇    下一篇

34种铁兰属植物亲缘关系的SRAP分析

宿静1,李玉萍2,汤庚国3   

  1. 1.东营市技师学院,山东东营257091;
    2.金陵科技学院园艺学院,江苏南京210038;
    3.南京林业大学森林资源与环境学院,江苏南京210037
  • 出版日期:2013-10-18 发布日期:2013-10-18
  • 基金资助:
    收稿日期:2012-11-08修回日期:2013-04-25 基金项目:江苏省镇江市科技计划项目(NY2007044) 第一作者:宿静,农艺师,博士。Email:sujing418@163.com。 引文格式:宿静,李玉萍,汤庚国. 34种铁兰属植物亲缘关系的SRAP分析[J]. 南京林业大学学报:自然科学版,2013,37(5):153-156.

Genetic relationship of 34 Tillandsia plants using SRAP marker

SU Jing1,LI Yuping2,TANG Gengguo3   

  1. 1.Technician College of Dongying, Dongying 257091,China;
    2.College of Horticulture, Jinling Institute Technology; Nanjing 210038,China;
    3. College of Forest Resources and Environment, Nanjing Forestry University, Nanjing 210037,China
  • Online:2013-10-18 Published:2013-10-18

摘要: 利用SRAP分子标记对34种铁兰属植物的亲缘关系进行分析。挑选了10对特异性引物,共扩增出402个位点,多态性位点376个,多态性百分率94%,相似系数0.134 3~0.955 6。聚类分析发现,34份铁兰属植物材料在相似系数为0.78时可划分为4个类群。分布地域是影响聚类结果的重要影响因子,实验证明Tillandsia juncea与T.subgen.Anoplophytum亚属有较近的亲缘关系。

Abstract: In this paper, SRAP was used to assess the genetic relationship of 34 Tillandsia varieties. Ten pairs of primers were selected. 402 bands were amplified, of which 376 bands(94%)were polymorphic, and the Nei′s genetic similarity coefficients ranged from 0.134 3-0.955 6. According to the UPGMA method,we made the cluster analysis by using the NTSYS software,34 Tillandsia plants could be divided into four groups at the similarity coefficient of 0.78.The geographical distribution was a principal factor for the cluster result. The experiment indicated that Tillandsia juncea and T. subgen. Anoplophytum had close relationship.

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