34种铁兰属植物亲缘关系的SRAP分析

宿静; 李玉萍; 汤庚国

doi:10.3969/j.issn.1000-2006.2013.05.029

34种铁兰属植物亲缘关系的SRAP分析

宿静,李玉萍,汤庚国

南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 153-156.

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南京林业大学学报（自然科学版） ›› 2013, Vol. 37 ›› Issue (05) : 153-156. DOI: 10.3969/j.issn.1000-2006.2013.05.029

研究简报

34种铁兰属植物亲缘关系的SRAP分析

宿静¹,李玉萍²,汤庚国³

作者信息 +

Genetic relationship of 34 Tillandsia plants using SRAP marker

SU Jing¹,LI Yuping²,TANG Gengguo³

Author information +

文章历史 +

摘要

利用SRAP分子标记对34种铁兰属植物的亲缘关系进行分析。挑选了10对特异性引物，共扩增出402个位点，多态性位点376个，多态性百分率94%，相似系数0.134 3～0.955 6。聚类分析发现，34份铁兰属植物材料在相似系数为0.78时可划分为4个类群。分布地域是影响聚类结果的重要影响因子，实验证明Tillandsia juncea与T.subgen.Anoplophytum亚属有较近的亲缘关系。

Abstract

In this paper, SRAP was used to assess the genetic relationship of 34 Tillandsia varieties. Ten pairs of primers were selected. 402 bands were amplified, of which 376 bands(94%)were polymorphic, and the Nei′s genetic similarity coefficients ranged from 0.134 3-0.955 6. According to the UPGMA method，we made the cluster analysis by using the NTSYS software，34 Tillandsia plants could be divided into four groups at the similarity coefficient of 0.78.The geographical distribution was a principal factor for the cluster result. The experiment indicated that Tillandsia juncea and T. subgen. Anoplophytum had close relationship.

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宿静,李玉萍,汤庚国. 34种铁兰属植物亲缘关系的SRAP分析[J]. 南京林业大学学报（自然科学版）. 2013, 37(05): 153-156 https://doi.org/10.3969/j.issn.1000-2006.2013.05.029

SU Jing,LI Yuping,TANG Gengguo. Genetic relationship of 34 Tillandsia plants using SRAP marker[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2013, 37(05): 153-156 https://doi.org/10.3969/j.issn.1000-2006.2013.05.029

中图分类号： Q948

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基金

收稿日期：2012-11-08修回日期：2013-04-25 基金项目：江苏省镇江市科技计划项目(NY2007044) 第一作者：宿静,农艺师,博士。Email:sujing418@163.com。引文格式：宿静,李玉萍,汤庚国. 34种铁兰属植物亲缘关系的SRAP分析[J]. 南京林业大学学报：自然科学版，2013，37（5）：153-156.