对欧洲鹅耳枥种子进行变温层积处理,测定种子发芽率,淀粉、可溶性糖、蛋白质、粗脂肪含量及相关酶的活性,研究欧洲鹅耳枥种子萌发前层积过程中主要贮藏物质的含量以及相应酶活性的动态变化。结果表明:在变温层积过程中, 欧洲鹅耳枥种子中淀粉、粗脂肪逐渐减少, 可溶性蛋白含量先增加后减少,层积结束时较对照分别减少了39.7%、92.2%、2.3%; 可溶性糖含量明显增加,较对照增加了129%; 相应酶活性呈增加趋势,淀粉酶、酸性磷酸酯酶及过氧化物酶活性较对照分别增加了550%、228%、67%; 变温层积150 d后种子发芽率大幅度升高。说明变温层积能有效解除欧洲鹅耳枥种子的休眠,贮藏物质不断分解代谢,为种子萌发做准备。
Abstract
In order to determine the changes in seed germination, biochemical composition and enzyme activity after alternative stratification, Carpinus betulus seeds were investigated. The results indicated that the content of starch and crude fat all gradually declined, the soluble protein climbed up first, but then declined, they respectively reduced 39.7%, 92.2%, 2.3% after alternative stratification, compared with CK; the content of soluble sugars obviously increased by 129%, the corresponding enzyme activity had an increase trendency, increased by 550%, 228%, 67%, compared with CK. After being laminated 150 d, the germination percentage increased greatly. The result indicated that alternative stratification could effectively break dormancy of Carpinus betulus L. seeds and the stored substance was continuously catabolism to prepare for the seeds germination in the process of alternative stratification.
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
参考文献
[1] Edward F Gilman, Dennis G Watson. Carpinus betulus[R]. Fact Sheet ST-118, 1993.
[2] Metzger F T. Carpinus caroliniana Walt[C]// Burns R M,Honkala B H. Silvics of North America(Volume 2). Hardwoods, Washington D C:USDA Forest Service,1990.
[3] Rudolf P O,Phipps H. Carpinus[C]// Schopmeyer C S. Seeds of Woody Plants in the United States. Washington D C:USDA Forest Service,1974.
[4] 国家林业局国有林场和林木种苗工作总站.世界园林乔灌木[M].北京:中国林业出版社,2004.
[5] 钱又宇,薛隽.世界著名观赏树木100种[M].武汉:武汉理工大学出版社,2006.
[6] Dirr M A. Manual of Woody Landscape Plants[M]. Champaign I L: Stipes Publishing,1900.
[7] Suszka B, Muller C, Bonnet-Masimbert M. Seeds of Forest Broad-Leaves: From Harvest to Sowin[M]. Paris:Institut National de la Recerche Agronomique,1998.
[8] Paula M.Carpinus L.[C]// Forest Service.The Woody Plant Seed Manual.Washington D C:USDA Forest Service,2008.
[9] 廖云娇,李雪,董学会.不同变温层积过程中东北红豆杉种子生理生化特性和胚形态的变化[J].中国农业大学学报,2010,15(1):39-44. Liao Y J, Li X, Dong X H. Effect of different temperature stratifications on physiological and biochemical characteristics and seed embryos development of Taxus cuspidata Sieb. et Zucc.[J].Journal of China Agricultural University, 2010,15(1):39-44.
[10] 北京师范大学生物系生化教研室.基础生物化学试验[M].北京:高等教育出版社,1982.
[11] 王家源,方升佐.青钱柳种子层积过程中贮藏物质含量及酶活性的变化[J]. 南京林业大学学报: 自然科学版,2007,31(1):111-113. Wang J Y, Fang S Z. Storage substance content and corresponding enzyme activity during the stratification of Cyclocarya paliurus seeds[J].Journal of Nanjing Forestry University:Natural Sciences Edition, 2007,31(1):111-113.
[12] Major W,Roberts E H.Dormancy in cereals.Ⅱ:The nature of gaseous exchange in imbibed barley and rice seeds[J].Journal of Experimental Botany,1968,19:90-101.
[13] 赵永华,刘惠卿,杨世林,等.西洋参种子休眠解除与磷酸戊糖途径关系的研究[J].中草药,2001, 32(3):259-261. Zhao Y H, Liu H Q, Yang S L, et al. Relationship between phosphopentose pathway and seed dormancy releasing of Panax quinquefolius[J].Chinese Traditional and Herbal Drugs, 2001, 32(3):259-261.
[14] 许岳香,黄 丹, 胡海波.美国桂花种子休眠原因的分析[J]. 南京林业大学学报: 自然科学版,2008,32(6):65-68. Xu Y X, Huang D, Hu H B. Analysis of the reasons for dormancy of Osmanthus americanus seed[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2008,32(6):65-68.
[15] Simmands J A,Simpson G M.Increased participation of pentose phosphare pathway in response to after ripening and gibberelic acid treatment in caryopses of Avena fatua[J]. Canadian J Botany, 1971, 49: 1843-1840.
基金
收稿日期:2012-11-20 修回日期:2013-03-27
基金项目:江苏省科技支撑计划(BE2012345); 国家林业局“948”项目(2011-4-44); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:祝遵凌,副教授,博士。E-mail: zhuzunling@aliyun.com。
引文格式:祝遵凌,林庆梅,许园园. 欧洲鹅耳枥种子层积过程中贮藏物质及酶活性的变化[J]. 南京林业大学学报:自然科学版,2013,37(6):157-160.
PDF(559692 KB)
