以3个欧美杂种山杨(Populus tremula×P.tremuloides)和1个中国山杨(P.davidiana)无性系1年生扦插幼苗为研究材料,利用人工气候室模拟自然降温过程,进行25、15、10、5、0 ℃的温度处理,测定了低温胁迫下杂种山杨无性系幼苗与抗寒性相关的生理生化指标的变化。结果表明:随着温度的降低,山杨无性系内源ABA含量、可溶性蛋白质和丙二醛(MDA)含量均呈上升趋势,超氧化物歧化酶(SOD)活性表现出先上升后下降再上升的趋势。杂种山杨无性系Ptr3和Ptr2在胁迫过程中SOD活性、可溶性蛋白和丙二醛含量相对较高,均高于中国山杨。由此表明,引进的欧美杂种山杨无性系表现出较高的抗寒性,适合在我国东北地区栽种。
Abstract
One year old aspen cuttings, including three hybrid clones of Populus tremula × P. tremuloides and one clone of P. davidiana, were tested for analyzing the changes of the physiological and biochemical indexes related to cold resistance of the clones from 25 ℃ to 15 ℃,10 ℃,5 ℃ and 0 ℃ using climate chamber to simulate natural temperature reduction.The results showed that the endogenous ABA content, soluble protein and malondialdehyde content(MDA)of the clones increased along the temperature cooling down,the activities of SOD of the clones trended in up and down first, and then up again. The endogenous ABA, soluble protein and MDA content of clone Ptr3 and Ptr2 were relatively higher than that of Chinese aspen during the treating process. Therefore, the hybrid aspen adapted to plant in northeast part of China because of good adaptability to the condition of introduction area.
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基金
收稿日期:2012-11-25 修回日期:2013-03-12
基金项目:东北林业大学林木遗传育种国家重点实验室开放基金项目(201106)
第一作者:闫绍鹏,工程师,博士。*通信作者:王秋玉,教授。E-mail: wqy11@sina.com。
引文格式:闫绍鹏,杨瑞华,王秋玉. 低温胁迫对欧美杂种山杨无性系的生理影响[J]. 南京林业大学学报:自然科学版,2013,37(6):161-164.
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