基于DNA的木材树种识别研究进展

doi:10.3969/j.issn.1000-2006.2014.01.027

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (01): 151-156.doi: 10.3969/j.issn.1000-2006.2014.01.027

基于DNA的木材树种识别研究进展

张蓉^1,2,殷亚方²,徐魁梧¹,叶克林^2,1*

1.南京林业大学材料科学与工程学院, 江苏南京 210037;
2.中国林业科学研究院木材工业研究所, 北京 100091

出版日期:2014-01-15 发布日期:2014-01-15
基金资助:
收稿日期:2013-03-14 修回日期:2013-06-20
基金项目:国家林业公益性行业科研专项项目(201304508); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:张蓉,博士生。*通信作者:叶克林,研究员。E-mail: yekelin@caf.ac.cn。
引文格式:张蓉,殷亚方,徐魁梧,等. 基于DNA的木材树种识别研究进展[J]. 南京林业大学学报:自然科学版,2014,38(1):151-156.

Progress of wood identification based on DNA methods

ZHANG Rong^1,2, YIN Yafang², XU Kuiwu¹,YE Kelin^2,1*

1. College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210037, China;
2. Research Institute of Wood Industry, Chinese Academy of Forestry, Beijing 100091,China

Online:2014-01-15 Published:2014-01-15

摘要/Abstract

摘要： 从木材识别的角度,对木材DNA提取方法(CTAB、SDS、PTB、DNeasy Plant Mini Kit等)和DNA条形码及DNA指纹图谱等基于DNA的木材树种识别方法进行了综合评述。其中,针对DNA条形码方法,重点阐述了基因组DNA中可用的特征序列来源叶绿体基因(chloroplast DNA, cpDNA)rbcL、matK、trnH-psbA间隔区序列,核糖体基因(ribosomal DNA, rDNA)ITS序列,以及变异位点的识别和系统进化树的应用等问题; 针对DNA指纹图谱方法,重点阐述了(RAPD、ISSR、SSR、SNP)4种DNA分子标记方法在DNA指纹图谱中的研究和应用现状。笔者认为,以DNA特征序列为依据的木材树种识别理论上虽然是可行的,但要应用于实际还需开展更多的研究。

Abstract: From wood identification points of view, DNA extraction methods(CTAB, SDS, PTB, DNeasy Plant Mini Kit etc.)and DNA barcode and fingerprint and wood identification methods based on DNA were summarized. For the DNA barcode concerned, the emphases were on the signature sequences in genome DNA(chloroplast DNA, cpDNArbcL,matK,trnH-psbAintergenic spacer sequences,and ribosomal DNA, rDNA ITS)as well as identification form(variable sites and phylogenetic tree)described. For the DNA fingerprint method,the emphases were on the progress and outlook of 4 kinds of molecular makers such as random amplificated polymorphism DNA, RAPD,inter-simple sequence repeat, ISSR、simple sequence repeats, SSR,single nucleotide polymorphism, SNP. It was concluded that wood identification based on DNA methods is feasible in theory but needs more studied on.

中图分类号:

S781

张蓉,殷亚方,徐魁梧,等. 基于DNA的木材树种识别研究进展[J]. 南京林业大学学报（自然科学版）, 2014, 38(01): 151-156.

ZHANG Rong, YIN Yafang, XU Kuiwu,YE Kelin. Progress of wood identification based on DNA methods[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(01): 151-156.DOI: 10.3969/j.issn.1000-2006.2014.01.027.

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基于DNA的木材树种识别研究进展

Progress of wood identification based on DNA methods

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