造纸白水中的细菌多样性及系统发育树分析

doi:10.3969/j.issn.1000-2006.2014.02.023

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (02): 123-127.doi: 10.3969/j.issn.1000-2006.2014.02.023

造纸白水中的细菌多样性及系统发育树分析

吴芳芳¹,程强²,时留新^3*

1.南京林业大学化学工程学院,江苏南京 210037;
2.南京林业大学,江苏省杨树种质创新与品种改良重点实验室, 江苏南京 210037;
3.南京林业大学轻工科学与工程学院,江苏南京 210037

出版日期:2014-03-10 发布日期:2014-03-10
基金资助:
收稿日期:2013-04-25 修回日期:2013-11-13
基金项目:国家林业局“948”项目(2012-4-09); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:吴芳芳,硕士生。*通信作者:时留新,副教授。E-mail: shiliuxin@njfu.edu.cn。
引文格式:吴芳芳,程强,时留新,等. 造纸白水中的细菌多样性及系统发育树分析[J]. 南京林业大学学报:自然科学版,2014,38(2):123-127.

Bacterial diversity and phylogenetic analysis of whitewater in papermaking process

WU Fangfang¹,CHENG Qiang²,SHI Liuxin^3*

1.College of Chemical Engineering, Nanjing Forestry University,Nanjing 210037,China;
2.Jiangsu Key Laboratory for Poplar Germplasm Enhancement and Variety Improvement, Nanjing Forestry University, Nanjing 210037, China;
3.College of Light Indu

Online:2014-03-10 Published:2014-03-10

摘要/Abstract

摘要： 在制浆造纸过程中,白水的循环回用加重了纸机微生物污染的问题,促进了纸机腐浆的形成,影响了纸机的正常运转。此次研究用滤膜抽滤富集细菌菌体,对白水样品直接提取DNA,通过16S rDNA序列分析,构建16S rDNA文库,对芬欧汇川(UPM)常熟纸厂PM1纸机白水中的细菌多样性进行了研究。获得的61个16S rDNA序列可分为28个可操作分类单元(OTUs),分属于6个不同门类,优势菌种顺序为变形菌门(Proteobacteria)(77.05%)、厚壁菌门(Firmicutes)(11.48%)、放线菌门(Actinobacteria)(4.92%)、异常球菌-栖热菌门(Deinococcus-Thermus)(3.28%)、拟杆菌门(Bacteroidetes)(1.64%)、蓝藻门(Cyanobacteria)(1.64%)。造纸白水具有较丰富的细菌多样性,以变形菌为优势菌群,占库容总量的77.05%,其中尤以β-变形菌群为主,占库容总量的45.90%,而首次在中国纸机鉴定出的水生施莱格尔氏菌(Schlegelella aquatica)为克隆文库的优势菌株,另外还含有2个菌种属于异常球菌-栖热菌门,该菌门为红色腐浆形成菌种。

Abstract: Paper machines offer a suitable environment for microbial growth, which results in slime problems during the process of papermaking. Investigation of the bacterial diversity in whitewater which recycled in paper mill UPM was carried out in this paper. Total DNA were directly extracted from the whitewater sample. 16S rDNA were amplified directly from purified DNA by PCR with universally bacteria-specific rDNA primers and cloned. Sixty-one bacteria were identified by partial sequencing of their 16S rDNAs. Twenty-eight operational taxonomic units(OTUs)were clustered in the following phyla including Proteobacteria(77.05%), Firmicutes(11.48%), Actinobacteria(4.92%), Deinococcus-Thermus(3.28%), Bacteroidetes(1.64%),Cyanobacteria(1.64%). Bacterial diversity was high in the whitewater sample. The results showed a high proportion of Schlegelella aquatica in the white water samples. It was the first time that Schlegelella aquatica strain had been identified from a paper machine in China. We also identified the red-slime formation bacteria Deinococcus-Thermus in whitewater sample.

中图分类号:

TS75
Q19

吴芳芳,程强,时留新. 造纸白水中的细菌多样性及系统发育树分析[J]. 南京林业大学学报（自然科学版）, 2014, 38(02): 123-127.

WU Fangfang,CHENG Qiang,SHI Liuxin. Bacterial diversity and phylogenetic analysis of whitewater in papermaking process[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(02): 123-127.DOI: 10.3969/j.issn.1000-2006.2014.02.023.

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造纸白水中的细菌多样性及系统发育树分析

Bacterial diversity and phylogenetic analysis of whitewater in papermaking process

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