Abstract: Using the young leaves of aseptic broad leaf evergreen rhododendron seedling as the starting materials for this experiment, the culture conditions in vitro and regeneration of adventitious budswere studied. The results showed that:the best medium composition inducing adventitious buds directly from leaf explants were MS +ZT 1.0 mg/L + NAA 0.1 mg/L, which made the induction rate reach to 70%. The adventitious buds could also be induced from the callus indirectly, the best medium composition for which was MS+2,4-D 2.0 mg/L+KT 0.2 mg/L, the best multiplication medium for callus was MS+ZT 0.5 mg/L+ IBA 0.1mg/L,the best medium for callus differentiation was MS+ZT 0.04 mg/L+NAA 0.01 mg/L, which made differentiation rate reach to 70% and the differentiation number of seedlings 6-10. The rate of rooting could reach to 90% by the medium of MS+NAA 0.2 mg/L +IBA 0.4 mg/L. The mixed compost contained four parts of humus soil, and one part of yellow sand, one part of perlite, which was quite suitable for evergreen rhododendron seedlings transplanting in vitro, and the survival rate was 90%.