以大叶常绿杜鹃无菌苗的嫩叶为试验起始材料,研究了其离体培养和不定芽再生的过程。结果表明:培养基为MS+ZT(1.0 mg/L)+ NAA(0.1 mg/L)时,大叶常绿杜鹃叶片可直接诱导再生不定芽,诱导率达70%; 也可以通过愈伤组织途径间接诱导出不定芽,愈伤组织诱导培养基为MS+2,4-D(2.0 mg/L)+KT(0.2 mg/L),增殖最佳培养基为MS+ZT(0.5 mg/L)+IBA(0.1 mg/L),愈伤组织分化的最佳培养基为MS+ZT(0.04 mg/L)+NAA(0.01 mg/L); 分化率最高为70%,分化苗数为6～10株; 生根培养基为MS+NAA(0.2 mg/L)+IBA(0.4 mg/L)时,生根率可达90%。腐殖土、黄沙、珍珠岩配比为4:1:1的混合基质较适合常绿杜鹃试管苗的炼苗移栽,成活率为90%。
Abstract
Using the young leaves of aseptic broad leaf evergreen rhododendron seedling as the starting materials for this experiment, the culture conditions in vitro and regeneration of adventitious budswere studied. The results showed that:the best medium composition inducing adventitious buds directly from leaf explants were MS +ZT 1.0 mg/L + NAA 0.1 mg/L, which made the induction rate reach to 70%. The adventitious buds could also be induced from the callus indirectly, the best medium composition for which was MS+2,4-D 2.0 mg/L+KT 0.2 mg/L, the best multiplication medium for callus was MS+ZT 0.5 mg/L+ IBA 0.1mg/L,the best medium for callus differentiation was MS+ZT 0.04 mg/L+NAA 0.01 mg/L, which made differentiation rate reach to 70% and the differentiation number of seedlings 6-10. The rate of rooting could reach to 90% by the medium of MS+NAA 0.2 mg/L +IBA 0.4 mg/L. The mixed compost contained four parts of humus soil, and one part of yellow sand, one part of perlite, which was quite suitable for evergreen rhododendron seedlings transplanting in vitro, and the survival rate was 90%.
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参考文献
[1] 张春英.杜鹃花的育种发展及现代育种[J].山东林业科技,2005(3):77-79.
Zhang C Y. Breeding development and contemporary breeding of Rhododendron[J].Shandong Forestry Science and Technology,2005(3):77-79.
[2] Walter Magor. A history of Rhododendron[J].American Rhododendron Society Notes, 2002:7-8.
[3] Anderson W C.A revised tissue culture medium for shoot multiplication of Rhododendron[J]. Journal of the American Society for Horticultural Science,1984,109:343-347.
[4] Read P E,Fellman C D.Accelerating acclimation of in vitro propagated woody ornamentals[J].Acta Horticulturae, 1985,166:15-20.
[5] 张杨军,涂艺声,彭先全,等.云锦杜鹃离体再生培养基的条件优化[J].安徽农业科学,2010,38(11):6008-6010.
Zhang Y J,Tu Y S,Peng X Q,et al.Research on the medium optimization for the plant regeneration in vitro of health family[J].Journal of Anhui Agri Sci, 2010,38(11):6008-6010.
[6] 刘燕,陈训.西洋杜鹃叶片离体培养及植株再生[J].种子,2008,27(11):46-50.
Liu Y,Chen X. Induction of callus from leaf explants of Rhododendron hybridum and its regeneration[J].Seed, 2008,27(11):46-50.
[7] 苗永美,王永清,庄平,等.桃叶杜鹃组织培养技术研究[J].生物学杂志,2006,23(6):29-32.
Miao Y M, Wang Y Q, Zhuang P,et al. Study on technique tissue culture in Rhododendron anne Franch[J].Journal of Biology, 2006,23(6):29-32.
[8] 王亚丽,王晓东,赵兵,等.光质对玛咖愈伤组织生长、分化的影响[J].过程工程学报,2007,7(4):782-785.
Wang Y L, Wang X D, Zhao B, et al.Effects of light quality on Maca callus growth and differentiation[J].The Chinese Journal of Process Engineering, 2007,7(4):782-785.
基金
收稿日期:2013-01-26 修回日期:2013-10-12
基金项目:国家自然科学基金项目(30972409)
第一作者:吕秀立,高级工程师。*通信作者:张春英,高级工程师,博士。E-mail: mayzhang55@163.com。
引文格式:吕秀立,魏翔莺,尹丽娟,等. 大叶常绿杜鹃叶片离体培养及不定芽再生[J]. 南京林业大学学报:自然科学版,2014,38(2):165-168.
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