雪落樱再生体系的建立

doi:10.3969/j.issn.1000-2006.2014.S1.005

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南京林业大学学报（自然科学版） ›› 2014, Vol. 38 ›› Issue (增刊): 20-24.doi: 10.3969/j.issn.1000-2006.2014.S1.005

雪落樱再生体系的建立

史港影,南程慧,伊贤贵,张开文,王贤荣^*

南京林业大学生物与环境学院,江苏南京 210037

出版日期:1900-01-01 发布日期:1900-01-01
基金资助:
收稿日期:2014-09-10
基金项目:江苏省科技支撑计划(BE2012346); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:史港影,硕士生。*通信作者:王贤荣,教授,博士。E-mail: wangxianrong66@nufu.edu.cn。
引文格式:史港影,南程慧,伊贤贵,等. 雪落樱再生体系的建立[J]. 南京林业大学学报:自然科学版,2014,38(S1):20-24.

Establishment of regeneration system in Cerasus xueluoensis

SHI Gangying, NAN Chenghui, YI Xiangui, ZHANG Kaiwen WANG Xianrong^*

College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China

Online:1900-01-01 Published:1900-01-01

摘要/Abstract

摘要： 以雪落樱(Cerasus xueluoensis)叶片为外植体,研究灭菌方式、不同激素配比组合及含糖量等因素对叶片再生的影响。结果表明,以春季充分展开的嫩叶为外植体,用0.1% HgCl₂灭菌7 min效果较理想。筛选出MS为最适的基本培养基,并在培养基中添加20 mg/L Vc溶液,能有效缓解愈伤组织继代过程中的褐变状况; 愈伤组织增殖以MS+6-BA(1.0 mg/L)+NAA(0.5 mg/L)+Vc(20 mg/L)为宜,增殖快速且生长良好; 在MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)+TDZ(2.0 mg/L)+Vc(20 mg/L)培养基中愈伤组织分化最理想,不定芽生长健壮; 将分化健壮的不定芽接种到3/4MS+NAA(0.1 mg/L)+蔗糖(15 g/L)的培养基中生根,27 d后生根率达100%。

Abstract: A plant regeneration system via adventitious shoot formation from leaf explants of Cerasus xueluoensis was established. Several factors such as explant sterilizing time, hormone concentration and concentration of sucrose and so on were explored to optimize the regeneration system in virto. The result showed that the fully unfold leaves were selected as the most appropriate explant in spring. And the best basic medium is MS. With 6-BA(1.0 mg/L)+NAA(0.5 mg/L)+Vc(20 mg/L)was the optimum medium for callus proliferation. And 20 mg/L Vc was best to inhibit C. xueluoensis callus from browning. MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)+TDZ(2.0 mg/L)+Vc(20 mg/L)was the optimum medium for shoot differentiation. And 3/4MS+NAA(0.1 mg/L)+sucrose(15 g/L)was the optimum medium for root differentiation. The rooting percentage was 100% after 27 days.

中图分类号:

S503.53

史港影,南程慧,伊贤贵,等. 雪落樱再生体系的建立[J]. 南京林业大学学报（自然科学版）, 2014, 38(增刊): 20-24.

SHI Gangying, NAN Chenghui, YI Xiangui, ZHANG Kaiwen WANG Xianrong. Establishment of regeneration system in Cerasus xueluoensis[J].Journal of Nanjing Forestry University (Natural Science Edition), 2014, 38(增刊): 20-24.DOI: 10.3969/j.issn.1000-2006.2014.S1.005.

参考文献

[1] 闫道良,王贤荣,钦佩,等. 钟花樱组织培养再生体系的建立[J]. 林业科技开发,2006,20(3):21-24.Yan D L,Wang X R,Qin P, et al. Regeneration system of tissue culture for Cerasus cerasoides var. campanulata[J]. China Forestry Science and Technology,2006, 20(3):21-24.
[2] 荣冬青,王贤荣. 垂枝早樱‘红枝垂’组培快繁试验[J]. 林业科技开发,2008,22(5):72-75.Rong D Q, Wang X R. A study on tissue culture and rapid propagation of Ceraus subhirtella var. pendul ‘Rosea’[J]. China Forestry Science and Technology,2008,22(5):72-75.
[3] 南程慧,王贤荣,汤庚国,等. 迎春樱组培无菌外植获得方法初探[J]. 湖北民族学院学报:自然科学版,2010,28(3):251-255.Nan C H, Wang X R, Tang G G, et al. Preliminary study on sterile explants of Cerasus discoidea[J]. Journal of Hubei Institute for Nationalities:Natural Science Edition,2010,28(3):251-255.
[4] Nan C H, Wang X R, Tang G G, et al. Cerasus xueluoensis(Rosaceae), a new species from China[J]. Annales Botanici Fennici,2013,55:79-82.
[5] 许红梅. 植物组织培养中的污染及防止措施[J]. 北方园艺,2006(6):148-149.Xu H M. Prevention and contamination in plant tissue culture[J]. Northern Horticulture, 2006(6):148-149.
[6] 杜雪玲,张振霞,余如刚,等.植物组织培养中的污染成因及其预防[J]. 草业科学,2005,22(1):24-27.Du X L, Zhang Z X, Yu R G, et al. The cause of contamination and its prevention in plant tissue culture[J]. Pratacultural Science, 2005, 22(1): 24-27.
[7] 徐振彪,傅作申,原亚萍,等. 植物组织培养过程中的褐化现象[J]. 国外农学:杂粮作物,1997(1):55-56.Xu Z B,Fu Z S,Yuan Y P, etal. The browning phenomenon in the process of plant tissue culture[J]. Foreign Agriculture:Coarse Grain Crops,1997(1):55-56.
[8] 谢志亮,吴振旺. 木本植物组培褐化研究进展[J]. 中国南方果树,2013,42(5):42-46. Xie Z L,Wu Z W. Resarch advances in browning of woody plant tissue culture[J]. South China Fruits, 2013, 42(5):42-46.
[9] 王瑞,陈永忠,王湘南,等. 碳源及蔗糖浓度对油茶愈伤组织诱导的影响[J]. 西南农业学报,2013,26(1):223-225.Huang R, Chen Y Z, Wang X N,et al. Effects of carbon sources and different concentration of sucroseon induction of Camellia oleifera callus[J]. Southwest China Journal of Agricultural Sciences, 2013, 26(1): 223-225.

雪落樱再生体系的建立

Establishment of regeneration system in Cerasus xueluoensis

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