以雪落樱(Cerasus xueluoensis)叶片为外植体,研究灭菌方式、不同激素配比组合及含糖量等因素对叶片再生的影响。结果表明,以春季充分展开的嫩叶为外植体,用0.1% HgCl2灭菌7 min效果较理想。筛选出MS为最适的基本培养基,并在培养基中添加20 mg/L Vc溶液,能有效缓解愈伤组织继代过程中的褐变状况; 愈伤组织增殖以MS+6-BA(1.0 mg/L)+NAA(0.5 mg/L)+Vc(20 mg/L)为宜,增殖快速且生长良好; 在MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)+TDZ(2.0 mg/L)+Vc(20 mg/L)培养基中愈伤组织分化最理想,不定芽生长健壮; 将分化健壮的不定芽接种到3/4MS+NAA(0.1 mg/L)+蔗糖(15 g/L)的培养基中生根,27 d后生根率达100%。
Abstract
A plant regeneration system via adventitious shoot formation from leaf explants of Cerasus xueluoensis was established. Several factors such as explant sterilizing time, hormone concentration and concentration of sucrose and so on were explored to optimize the regeneration system in virto. The result showed that the fully unfold leaves were selected as the most appropriate explant in spring. And the best basic medium is MS. With 6-BA(1.0 mg/L)+NAA(0.5 mg/L)+Vc(20 mg/L)was the optimum medium for callus proliferation. And 20 mg/L Vc was best to inhibit C. xueluoensis callus from browning. MS+6-BA(2.0 mg/L)+NAA(0.1 mg/L)+TDZ(2.0 mg/L)+Vc(20 mg/L)was the optimum medium for shoot differentiation. And 3/4MS+NAA(0.1 mg/L)+sucrose(15 g/L)was the optimum medium for root differentiation. The rooting percentage was 100% after 27 days.
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基金
收稿日期:2014-09-10
基金项目:江苏省科技支撑计划(BE2012346); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:史港影,硕士生。*通信作者:王贤荣,教授,博士。E-mail: wangxianrong66@nufu.edu.cn。
引文格式:史港影,南程慧,伊贤贵,等. 雪落樱再生体系的建立[J]. 南京林业大学学报:自然科学版,2014,38(S1):20-24.
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