南京林业大学学报(自然科学版) ›› 2015, Vol. 39 ›› Issue (02): 183-186.doi: 10.3969/j.issn.1000-2006.2015.02.032

• 研究简报 • 上一篇    

木槿属4种植物花粉活力和杂交亲和性研究

李玉萍1,汤庚国2,罗凤霞1*   

  1. 1.金陵科技学院园艺学院,江苏 南京 210038;
    2.南京林业大学生物与环境学院,江苏 南京 210037
  • 出版日期:2015-03-31 发布日期:2015-03-31
  • 基金资助:
    收稿日期:2014-01-25 修回日期:2014-06-06
    基金项目:金陵科技学院重点学科建设工程资助项目
    第一作者:李玉萍,副教授,博士。*通信作者:罗凤霞,教授。E-mail: luofx@jit.edu.cn。
    引文格式:李玉萍,汤庚国,罗凤霞. 木槿属4种植物花粉活力和杂交亲和性研究[J]. 南京林业大学学报:自然科学版,2015,39(2):183-186.

Studies on pollen viability and cross-compatibility of four species of Hibiscus

LI Yuping1, TANG Gengguo2, LUO Fengxia1*   

  1. 1.College of Horticulture,Jinling Institute of Technology,Nanjing 210038, China;
    2.College of Biology and the Environment, Nanjing Forestry University, Nanjing 210037, China
  • Online:2015-03-31 Published:2015-03-31

摘要: 以木芙蓉、木槿、朱槿和重瓣朱槿为材料,对其花粉活力、花粉贮藏方法、杂交亲和性,以及杂交幼胚无菌培养进行研究。结果表明:①4种植物的花粉均具有较高的萌发率,依次为木芙蓉88.3%、重瓣朱槿82.5%、朱槿77.8%、木槿76.1%; ②室温和4 ℃不适宜该属植物花粉的贮藏,花粉活力最长保持2 d; ③木槿属种间杂交亲和性较低,木芙蓉×重瓣朱槿、木芙蓉×朱槿、木芙蓉×木槿的结实率分别为12%、8%、3%; ④木芙蓉×朱槿杂交组合获得的种子幼胚离体培养适宜培养基为 WPM+IBA 1.0 mg/L+BA 0.1 mg/L,此条件下的萌发率为56.6%。

Abstract: Pollen viability, storage method, crossing incompatibilities and young embryo culture were investigated for four Hibiscus species, e.g. H. mutabilis f. mutabilis, H. syriacus var. syriacus, H. rosa-sinensis var. rosa-sinensis, and H. roses-sinensis var. rubro-plenus, so as to provide the basis in the cultivation of new varieties of Hibiscus. The results were shown as follows: ① The pollen germination rate of all four species was relatively high. It could be arranged as: H. mutabilis f. mutabilis 88.3%, H. roses-sinensis var. rubro-plenus 82.5%, H. rosa-sinensis var. rosa-sinensis 77.8%, H. syriacus var. syriacus 76.1%; ② The pollen was not appropriate to storage at room temperature and 4 ℃, and the longest storage time was 2 d; ③ The cross incompatibilities between Hibiscus species was low and the seed setting rate was 12% for H. mutabilis f. mutabilis ×H. roses-sinensis var. rubro-plenus, 8% for H. mutabilis f. mutabilis ×H. rosa-sinensis var. rosa-sinensis, and 3% for H. mutabilis f. mutabilis ×H. syriacus var. syriacus; ④ the appropriate medium of young embryo of H. mutabilis f. mutabilis ×H. rosa-sinensis var. rosa-sinensis in vitro culture was WPM+IBA 1.0 mg/L+BA 0.1 mg/L, the germination rate was 56.6%.

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