两个桂花品种花色色素相关基因的差异表达

doi:10.3969/j.issn.1000-2006.2015.03.033

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (03): 183-186.doi: 10.3969/j.issn.1000-2006.2015.03.033

• 研究简报 • 上一篇

两个桂花品种花色色素相关基因的差异表达

母洪娜^1,2,3,孙陶泽³, 杨秀莲^1,2,王良桂^1,2*

1.南京林业大学风景园林学院,江苏南京 210037;
2.南京林业大学南方现代林业协同创新中心,江苏南京 210037;
3.长江大学园艺园林学院,湖北荆州 434025

出版日期:2015-05-30 发布日期:2015-05-30
基金资助:
收稿日期:2014-10-23 修回日期:2015-02-02
基金项目:国家林业公益性行业科研专项项目(201204607); 江苏省普通高校研究生科研创新计划资助项目(CXZZ13-0553); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:母洪娜,讲师。*通信作者:王良桂,教授。E-mail: wlg@njfu.com.cn。
引文格式:母洪娜,孙陶泽, 杨秀莲,等. 两个桂花品种花色色素相关基因的差异表达[J]. 南京林业大学学报:自然科学版,2015,39(3):183-186.

Differential expression of flower color related genes of Osmanthus fragrans Lour. ‘Chenghongdangui’ and ‘Zaoyingui’

MU Hongna^{1, 2,3},SUN Taoze³,YANG Xiulian^1,2, WANG Lianggui^1,2*

1. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China;
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
3.College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China

Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要： 结合桂花花青素、类胡萝卜素的含量与转录组测序检测到的花色代谢相关基因的差异表达,对品种为‘橙红丹桂’和‘早银桂’的花色变化过程进行了初步研究。结果发现:苯丙氨酸裂解酶、4-香豆酸—CoA 连接酶、乙酰辅酶A合酶、查耳酮合酶、R2R3-MYB1、细胞色素P450、番茄红素ε环化酶基因的表达与花青素和类胡萝卜素含量变化相似,这两类色素代谢路径上的其他基因均在‘早银桂’中表达量较高。此外,这种现象很可能是多酚氧化酶(PPO)、细胞色素P450基因在转录因子R2R3-MYB调控下差异表达所致,并且还发现桂花类黄酮的代谢路径是从柚皮素开始转向二氢槲皮素进而在DFR、ANS催化下形成矢车菊色素。

Abstract: The analysis of anthocyanin, carotenoid content and flower color gene expression of Osmanthus fragrans transcriptome between ‘Chenghongdangui’ and ‘Zaoyingui’ were carried out. Some genes such as phenylalanine ammonia lyase, 4-CoA ligasecoumaric acid, acetyl CoA synthase, chalcone synthase, R2R3-MYB1, cytochrome P450, lycopene epsilon cyclase did present up regulation in ‘Chenghongdangui’ petals not ‘Zaoyinggui’, which was consistent with the anthocyanin and carotenoid content in ‘Chenghongdangui’ and ‘Zaoyinggui’. In contrast, the expression profile of other genes was up regulation in ‘Zaoyingui. In addition, polyphenol oxidase(PPO), cytochrome P450 gene and R2R3-MYB that probably regulated pigments accumulation of O.fragrans were analyzed in this article. Meanwhile the novel discovery that the flavonoids metabolic pathway direct to dihydroquercetin at metabolism web junction, that is naringenin, and then synthesized cyanidin with catalyzing of DFR and ANS was found out in this study.

中图分类号:

S718.4

母洪娜,孙陶泽,杨秀莲,等. 两个桂花品种花色色素相关基因的差异表达[J]. 南京林业大学学报（自然科学版）, 2015, 39(03): 183-186.

MU Hongna^{1, 2,3},SUN Taoze,YANG Xiulian, WANG Lianggui. Differential expression of flower color related genes of Osmanthus fragrans Lour. ‘Chenghongdangui’ and ‘Zaoyingui’[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 39(03): 183-186.DOI: 10.3969/j.issn.1000-2006.2015.03.033.

参考文献

[1] 乔小燕,马春雷,陈亮.植物类黄酮生物合成途径及重要基因的调控[J].天然产物研究与开发, 2009(21):354-360,207. Qiao X Y, Ma C L, Chen L. Plant flavonoid biosynthesis pathway and regulation of its important genes[J]. Natural Product Research and Development, 2009(21): 354-360,207.
[2] Ronald Koes, Walter Verweij, Francesca Quattrocchio. Flavonoids: a colorful model for the regulation and evolution of biochemical pathways [J]. Trends in Plant Science, 2005,10(15):236-242.
[3] Sommer H, Saedler H. Structure of the chalcone synthase gene of Antirrhinum majus [J]. Molecular and General Genetics, 1986, 202(3): 429-434.
[4] Nakatsuka A, Izumi Y, Yamagishi M. Spatial and temporal expression of chalcone synthase and dihydroflavonol 4-reductase genes in the Asiatic hybrid lily [J]. Plant Science, 2003,165(4): 759-767
[5] 吴琦, 李成磊, 陈惠, 等. 苦荞查尔酮合酶基因CHS 的结构及花期不同组织表达量分析[J].中国生物化学与分子生物学报, 2010, 26(12):1151-1160. Wu Q,Li C L,Chen H,et al. Molecular structure characterization of Fagopyrum tataricum chalcone synthase and its tissue-specific expression during florescence[J]. Chinese Journal of Biochemistry and Molecular Biology, 2010, 26(12):1151-1160.
[6] Feinbaum R L, Ausubel F M. Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene [J]. Molecular and Cell Biology, 1988, 8(5): 1985-1992
[7] 李鹏.粘毛黄芩CHS基因植物表达载体的构建及其在烟草中的功能验证[D].重庆:西南大学,2010.Li P. Constructing of plant expression vectors of chalcone synthase gene form Scutellaria viseidula Bunge and its function verification in tobacco [D]. Chongqing: Southwest University,2010.
[8] 张大生,崔丽洁,王景明,等. 红巴梨F3H基因的克隆及植物表达载体的构建[J]. 南京林业大学学报:自然科学版,2005,29(2):65-68. Zhang D S,Cui L J,Wang J M, et al. Cloning F3H gene from red ballet fruit and its construction of plant express vector[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2005, 29(2): 65-68.
[9] 杨红丽,王彦昌,姜正旺,等. ‘红阳’ 猕猴桃cDNA文库构建及F3H基因的表达初探[J].遗传,2009,31(12):1265-1272. Yang H L, Wang Y C, Jiang Z W, et al. Construction of cDNA library of ‘Hongyang’ kiwifruit and analysis of F3H expression[J]. Hereditas, 2009, 31(12): 1265-1272.
[10] 蔡璇,苏蘩,金荷仙,等.四季桂花瓣色素的初步鉴定与提取方法[J].浙江林学院学报,2010,27(4):559-564. Cai X, Su F, Jin H X, et al. Components and extraction methods for petal pigments of Osmanthus fragrans ‘Siji Gui’[J]. Journal of Zhejiang Forestry College, 20l0, 27(4):559-564.
[11] 陈洪国. 桂花开花进程中花瓣色素、可溶性糖和蛋白质含量的变化[J]. 武汉植物学研究,2006,24(3):231-234. Chen H G. Changes of pigment, soluble sugar and protein content of Peta during florescence and senescence of Osmanthus fragrans Lour. [J]. Journal of Wuhan Botanical Research, 2006, 24(3): 231-234.
[12] 刘桂玲,李海霞,郭宾会,等. 不同提取方法对甘薯花青素含量测定的影响[J].中国农学通报,2007, 23(4):91-94. Liu G L, Li H X, Guo B H, et al. Effects of different extraction methods on anthocyanin content detection in Sweet Potato[J]. Chinese Agricultural Science Bulletin, 2007, 23(4):91-94.
[13] 颜少宾,张妤艳,马瑞娟,等.杏果肉中类胡萝卜素的提取方法研究[J].江西农业学报,2011,23(9):159-161. Yan S B, Zhang Y Y, Ma R J,et al. Study on extraction method of carotenoid from apricot fruit [J]. Acta Agriculturae Jiangxi, 2011,23(9): 159-161.
[14] 张岩,时威,吴燕燕,等. 白莲花粉中β-胡萝卜素的提取工艺条件的优化[J].食品与发酵科技,2011, 47(30):68-71. Zhang Y, Shi W, Wu Y Y, et al. Optimization of extraction conditions of β-Carotene in white lotus pollen[J]. Food and Fermentation Technology, 2011,47(30):68-71.
[15] Mu H N, Li H G, Wang L G, et al. Transcriptome sequencing and analysis of sweet osmanthus(Osmanthus fragrans Lour.)[J]. Genes and Genomics, 2014,36:777-788.
[16] 江曼. 下调GhDFR和GhF3H基因对棉花生长发育的影响[D]. 重庆:西南大学,2013.Jiang M. Down-Regulation of GhDFR and GhF3H Influence the growth and development of Cotton(Gossypium hirsutum L.)[D]. Chongqing: Southwest University, 2013.
[17] Ralf Stracke,Martin Werber, Bernd Weisshaar. The R2R3-MYB gene family in Arabidopsis thalialiana[J]. Current Opinion in Plant Biology, 2001, 4(5): 447-456.
[18] 牛姗姗. MYB对杨梅果实花青素苷合成的调控及其机制[D].杭州:浙江大学,2011. Niu S S. Regulation of anthocyanin biosynthesis by MYB in Chinese bayberry fruit and the mechanisms [D]. Hangzhou: Zhejiang University, 2011.
[19] Sornkanok Vimolmangkang, Han Y P, Wei G C, et al. An apple MYB transcription factor, MdMYB3, is involved in regulation of anthocyanin biosynthesis and flower development [J].BMC Plant Biology, 2013, 13(1):176.
[20] Lena G Fraser, Alan G Seal, Mirco Montefiori, et al. An R2R3 MYB transcription factor determines red petal colour in an Actinidia(kiwifruit)hybrid population [J]. BMC Genomics 2013, 14: 28.
[21] Nakayama T, Yonekur-askakibara K, Sato T, et al. Aureusidin synthase: a polyphenol oxidase homolog responsible for flower coloration [J]. Science, 2000, 290: 1163-1166.
[22] Strack D, Schliemann W. Bifunctional polyphenol oxidase: novel function in plant pigment biosynthesis [J]. Angewandte Chemie:International Edition, 2001,40(20):3791-3794.
[23] 贺丽虹,赵淑娟,胡之壁. 植物细胞色素P450基因与功能研究进展[J].药物生物技术, 2008, 15(2):142-147. He L H, Zhao S J, Hu Z B. Gene and function reasearch progress of plant cytochrome P450[J]. Pharmaceutical Biotechnology 2008, 15(2):142-147.

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两个桂花品种花色色素相关基因的差异表达

Differential expression of flower color related genes of Osmanthus fragrans Lour. ‘Chenghongdangui’ and ‘Zaoyingui’

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