两个桂花品种花色色素相关基因的差异表达

doi:10.3969/j.issn.1000-2006.2015.03.033

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (03): 183-186.doi: 10.3969/j.issn.1000-2006.2015.03.033

• 研究简报 • 上一篇

两个桂花品种花色色素相关基因的差异表达

母洪娜^1,2,3,孙陶泽³, 杨秀莲^1,2,王良桂^1,2*

1.南京林业大学风景园林学院,江苏南京 210037;
2.南京林业大学南方现代林业协同创新中心,江苏南京 210037;
3.长江大学园艺园林学院,湖北荆州 434025

出版日期:2015-05-30 发布日期:2015-05-30
基金资助:
收稿日期:2014-10-23 修回日期:2015-02-02
基金项目:国家林业公益性行业科研专项项目(201204607); 江苏省普通高校研究生科研创新计划资助项目(CXZZ13-0553); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:母洪娜,讲师。*通信作者:王良桂,教授。E-mail: wlg@njfu.com.cn。
引文格式:母洪娜,孙陶泽, 杨秀莲,等. 两个桂花品种花色色素相关基因的差异表达[J]. 南京林业大学学报:自然科学版,2015,39(3):183-186.

Differential expression of flower color related genes of Osmanthus fragrans Lour. ‘Chenghongdangui’ and ‘Zaoyingui’

MU Hongna^{1, 2,3},SUN Taoze³,YANG Xiulian^1,2, WANG Lianggui^1,2*

1. College of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China;
2. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
3.College of Horticulture and Gardening, Yangtze University, Jingzhou 434025, China

Online:2015-05-30 Published:2015-05-30

摘要/Abstract

摘要： 结合桂花花青素、类胡萝卜素的含量与转录组测序检测到的花色代谢相关基因的差异表达,对品种为‘橙红丹桂’和‘早银桂’的花色变化过程进行了初步研究。结果发现:苯丙氨酸裂解酶、4-香豆酸—CoA 连接酶、乙酰辅酶A合酶、查耳酮合酶、R2R3-MYB1、细胞色素P450、番茄红素ε环化酶基因的表达与花青素和类胡萝卜素含量变化相似,这两类色素代谢路径上的其他基因均在‘早银桂’中表达量较高。此外,这种现象很可能是多酚氧化酶(PPO)、细胞色素P450基因在转录因子R2R3-MYB调控下差异表达所致,并且还发现桂花类黄酮的代谢路径是从柚皮素开始转向二氢槲皮素进而在DFR、ANS催化下形成矢车菊色素。

Abstract: The analysis of anthocyanin, carotenoid content and flower color gene expression of Osmanthus fragrans transcriptome between ‘Chenghongdangui’ and ‘Zaoyingui’ were carried out. Some genes such as phenylalanine ammonia lyase, 4-CoA ligasecoumaric acid, acetyl CoA synthase, chalcone synthase, R2R3-MYB1, cytochrome P450, lycopene epsilon cyclase did present up regulation in ‘Chenghongdangui’ petals not ‘Zaoyinggui’, which was consistent with the anthocyanin and carotenoid content in ‘Chenghongdangui’ and ‘Zaoyinggui’. In contrast, the expression profile of other genes was up regulation in ‘Zaoyingui. In addition, polyphenol oxidase(PPO), cytochrome P450 gene and R2R3-MYB that probably regulated pigments accumulation of O.fragrans were analyzed in this article. Meanwhile the novel discovery that the flavonoids metabolic pathway direct to dihydroquercetin at metabolism web junction, that is naringenin, and then synthesized cyanidin with catalyzing of DFR and ANS was found out in this study.

中图分类号:

S718.4

母洪娜,孙陶泽,杨秀莲,等. 两个桂花品种花色色素相关基因的差异表达[J]. 南京林业大学学报（自然科学版）, 2015, 58(03): 183-186.

MU Hongna^{1, 2,3},SUN Taoze,YANG Xiulian, WANG Lianggui. Differential expression of flower color related genes of Osmanthus fragrans Lour. ‘Chenghongdangui’ and ‘Zaoyingui’[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(03): 183-186.DOI: 10.3969/j.issn.1000-2006.2015.03.033.

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两个桂花品种花色色素相关基因的差异表达

Differential expression of flower color related genes of Osmanthus fragrans Lour. ‘Chenghongdangui’ and ‘Zaoyingui’

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