Abstract: Four perennial flowering plants of Hemerocallis middendorfii, Hemerocallis×hybrida‘stella deoro’,Hosta‘so sweet’ and Hosta ensata were selected for a pot soil experiment combined with subcellular fractionation and chemical speciation sequential extraction techniques. The purpose was to study the accumulation characteristics, chemical forms and subcellular distribution of lead in the four plants. The results showed that the content of Pb in the underground part is more than that of in the aboveground part of the four flowers. All of the four plants emerged a maximum absorption content along with increasing the Pb treating time, but the time of reaching maximum absorption was different, and the transfer coefficient of the four flowers all reduced. A difference of Pb distribution and its chemical forms in the subcell of the four flowers was observed. Most of Pb was located in the vacuolus and cellular soluble parts, then in the cell wall and its residue and less Pb in the cell membranes and organelles. Pb in the four plants predominated HCl extractable, but the distribution forms of lead were different, the proportion of water and ethanol extractable northeast Pb was higher in Hosta ensata than that of in the other plants. This showed that the chemical form of Pb was related to the plan species, and also indicated Hosta ensata was likely to possess a higher tolerance to Pb.