拟松材线虫不同致病力虫株体内细菌的种类差异

doi:10.3969/j.issn.1000-2006.2015.04.011

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (04): 63-68.doi: 10.3969/j.issn.1000-2006.2015.04.011

拟松材线虫不同致病力虫株体内细菌的种类差异

袁为敏^1,2,项杨^1,2,吴小芹^1,2*

1.南京林业大学南方现代林业协同创新中心,江苏南京 210037;
2.江苏省有害生物入侵预防与控制重点实验室,南京林业大学林学院,江苏南京 210037

出版日期:2015-07-31 发布日期:2015-07-31
基金资助:
收稿日期:2014-06-30 修回日期:2014-12-01基金项目:国家自然科学基金项目(31270683); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:袁为敏,硕士生。*通信作者:吴小芹,教授。E-mail: xqwu@njfu.edu.cn。引文格式:袁为敏,项杨,吴小芹. 拟松材线虫不同致病力虫株体内细菌的种类差异[J]. 南京林业大学学报:自然科学版,2015,39(4):63-68.

Endobacteria from different virulence Bursaphelenchus mucronatus

YUAN Weimin^1,2, XIANG Yang^1,2, WU Xiaoqin^1,2*

1.Co-Innovation Center of Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China;
2.Jiangsu Key Laboratory of Pest Prevention and Control,College of Forestry, Nanjing Forestry University,Nanjing 210037,China

Online:2015-07-31 Published:2015-07-31

摘要/Abstract

摘要： 拟松材线虫(Bursaphelenchus mucronatus)为伞滑刃属植物寄生线虫,在形态上与引起松树萎蔫病(Pine Wild Disease)的松材线虫(B. xylophilus)极其相似。为探讨拟松材线虫的致病力与其体内细菌的关系,笔者将来自中国7省市不同地区的10个拟松材线虫虫株接种黑松测定其致病力,并对拟松材线虫的体内细菌进行了分离鉴定。结果表明,供试的10个拟松材线虫虫株中有6个虫株对黑松具致病力,4个虫株无致病力。通过分离拟松材线虫的体内细菌,发现有9个虫株体内存在细菌,共分离获得17株细菌,仅有1个虫株体内未分离到细菌。综合细菌菌落形态、生理生化指标、Biolog 96 孔碳源及16S rDNA序列分析对分离所得细菌进行了鉴定,结果从不同拟松材线虫虫株体内共鉴定出9属9种细菌,分别为嗜麦芽窄食单胞菌(Stenotrophomonas maltophilia)、美洲爱文氏菌(Ewingella americana)、荧光假单胞菌(Pseudomonas fluorescens)、根癌农杆菌(Agrobacterium tumefaciens)、Luteibacter anthropi、氧化微杆菌(Microbacterium oxydans)、Agreia pratensis、长野雷弗森菌(Leifsonia naganoensis),以及木糖氧化产碱菌木糖氧化亚种(Achromobacter xylosoxidans ssp. xylosoxidans)。对黑松有致病力的拟松材线虫多数虫株体内细菌的种类及数量多于无致病力的虫株,且能分离到嗜麦芽窄食单胞菌(S.maltophilia)的多数虫株均具有致病力,无致病力虫株的体内细菌主要为氧化微杆菌(M. oxydans)等。

Abstract: Bursaphelenchus mucronatus is one of plant parasitic nematodes and similar to the causative agent of pine wilt disease B. xylophilus in morphology. To understand the interaction between B. mucronatus and endobacteria, ten strains of B. mucronatus from seven provinces were inoculated to 2-year old Pinus thunbergii to evaluate their virulence. Besides, endobacteria of B. mucronatus were also isolated and analyzed. Pathogenic measurement showed that six strains of B. mucronatus were pathogenic to P.thunbergii, but the other four strains had no pathogenicity. Seventeen strains of endobacteria were isolated from 9 of 10 B. mucronatus strains. Physiological and biochemical characteristics, biolog methods combined with molecular revealed that seventeen strains of endobacteria belonged to Stenotrophomonas maltophilia, Ewingella americana, Pseudomonas fluorescens, Agrobacterium tumefaciens,Luteibacter anthropi, Microbacterium oxydans, Agreia pratensis, Leifsonia naganoensis and Achromobacter xylosoxidans ssp. xylosoxidans. Further analysis showed that species and population of endobacteria strains from virulent B. mucronatus strains were more abundant than that from non-virulent strains. S. maltophilia were usually isolated from virulent B. mucronatus strains, while endobacteria M. oxydans were mainly isolated from avirulent strains.

中图分类号:

S763.16

袁为敏,项杨,吴小芹. 拟松材线虫不同致病力虫株体内细菌的种类差异[J]. 南京林业大学学报（自然科学版）, 2015, 58(04): 63-68.

YUAN Weimin, XIANG Yang, WU Xiaoqin. Endobacteria from different virulence Bursaphelenchus mucronatus[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(04): 63-68.DOI: 10.3969/j.issn.1000-2006.2015.04.011.

参考文献

[1] 杨宝君. 松材线虫病 [M]. 北京:中国林业出版社, 2003.Yang B J. Pine wilt disease[M]. Beijing: Chinese Forestry Publishing House, 2003.
[2] Mota M M, Braasch H, Bravo M A, et al. First report of Bursaphelenchus xylophilus in Portugal and in Europe [J]. Nematology, 1999, 1(7): 727-734.
[3] Fonseca L, Cardoso J, Lopes A, et al. The pinewood nematode, Bursaphelenchus xylophilus, in Madeira Island [J]. Helminthologia, 2012, 49(2): 96-103.
[4] Abelleira A, Picoaga A, Mansilla A, et al. Detection of Bursaphelenchus xylophilus, causal agent of pine wilt disease on Pinus pinaster in Northwestern Spain [J]. Florida Entomologist, 2013, 96(3): 770-780.
[5] Cheng X Y, Xie P Z, Cheng F X, et al. Competitive displacement of the native species Bursaphelenchus mucronatus by an alien species Bursaphelenchus xylophilus(Nematoda: Aphelenchida: Aphelenchoididae): a case of successful invasion [J]. Biological Invasions, 2009, 11(2): 205-213.
[6] Rutherford T, Riga E, Webster J. Temperature-mediated behavioral relationships in Bursaphelenchus xylophilus, B. mucronatus, and their hybrids [J]. Journal of Nematology, 1992, 24(1): 40.
[7] Jikumaru S, Togashi K. Boarding abilities of Bursaphelenchus mucronatus and B. xylophilus (Nematoda: Aphelenchoididae)on Monochamus alternatus(Coleoptera: Cerambycidae)[J]. Nematology, 2003, 5(6): 843-849.
[8] Mamiya Y, Enda N. Bursaphelenchus mucronatus n. sp.(Nematoda: Aphelenchoididae)from pine wood and its biology and pathogenicity to pine trees [J]. Nematologica, 1979, 25(3):353-361.
[9] Tomminen J. Pathogenicity studies with Bursaphelenchus mucronatus in Scots pine in Finland [J]. European Journal of Forest Pathology, 1993, 23(4): 236-243.
[10] 程瑚瑞, 林茂松, 钱汝驹. 拟松材线虫(Bursaphelenchus mucronatus)的形态诊断和致病性研究 [J]. 南京农业大学学报, 1986,5(2):55-61.Cheng H R, Lin M S, Qian R J. A study on the morohological diagnosis and the pathogenicity of Bursaphelenchus mucronatus[J]. Journal of Nanjing Agricultural University, 1986,5(2):55-61.
[11] Braasch H, Caroppo S, Ambrogioni L, et al. Pathogenicity of various Bursaphelenchus species to pines and implications to European forests[C]//Kazuyoshi F, Katsumi T, Takefumi I. Sustainability of pine forests in relation to pine wilt and decline. Proceedings of International Symposium. Tokyo: Shokado, 1999.
[12] Bakke A, Anderson R, Kvamme T. Pathogenicity of the nematodes Bursaphelenchus xylophilus and B. mucronatus to Pinus sylvestris seedlings: a greenhouse test [J]. Scandinavian Journal of Forest Research, 1991, 6(1-4): 407-12.
[13] Kanzaki N, Futai K. Is Bursaphelenchus mucronatus a weak pathogen to the Japanese red pine? [J]. Nematology, 2006, 8(4): 485-489.
[14] Kawazu K, Kaneko N. Asepsis of the pine wood nematode isolate OKD-3 causes it to lose its pathogenicity [J]. Japanese Journal of Nematology, 1997, 27(2): 76-80.
[15] Kawazu K, Yamashita H, Kobayashi A,et al. Isolation of pine-wilting bacteria accompanying pine(Pinus)wood nematode, Bursaphelenchus xylophilus, and their toxic metabolites [J]. Scientific Reports of the Faculty of Agriculture-Okayama University, 1998, 87:1-7.
[16] 赵博光, 刘玉涛, 林峰. 松材线虫与其携带细菌之间的相互影响 [J]. 南京林业大学学报: 自然科学版, 2005, 29(3): 1-4.Zhao B G, Liu Y T, Lin F. Mutual influences between Bursaphelenchus xylophilus and bacteria carries[J]. Journal of Nanjing Forestry University:Natural Sciences Edition, 2005, 29(3): 1-4.
[17] 谈家金, 向红琼, 冯志新. 松材线虫伴生细菌的分离鉴定及其致病性 [J]. 林业科技开发, 2008, 22(2): 23-6.Tan J J, Xiang H Q, Feng Z X. A preliminary study on isolation,identification and pathogenicity of the bacterium accompanying Bursaphelenchus xylophilus [J]. China Forestry Science and Technology, 2008, 22(2): 23-6.
[18] Wu X Q, Yuan W M, Tian X J, et al. Specific and functional diversity of endophytic bacteria from pine wood nematode Bursaphelenchus xylophilus with different virulence [J]. International Journal of Biological Sciences, 2013, 9(1): 34-44.
[19] 袁为敏, 吴小芹, 叶建仁,等. 松材线虫和拟松材线虫体内细菌的透射电镜观察及分离鉴定[J]. 微生物学报, 2011, 51(8): 1071-1077.Yuan W M, Wu X Q, Ye J R, et al. Observation by transmission electron microscope andidentification of endophytic bacteria isolated from Bursaphelenchus xylophilus and B. mucronatus[J]. Acta Microbiologica Sinica, 2011, 51(8): 1071-1077.
[20] Dong X Z, Cai M Y. Identification system manual of common bacteria[J]. Beijing: Science Press, 2001.
[21] 张治宇,张克云,林茂松,等. 不同松材线虫群体对黑松的致病性测定 [J]. 南京农业大学学报, 2002, 25(2): 43-46.Zhang Z Y, Zhang K Y, Lin M S, et al. phthogenticity determination of Bursanhelenchus xylophilus to pine thunbergii[J]. Journal of Nanjing Agricultural University,2002,25(2):43-46.
[22] 张兴华. 拟松材线虫体表微生物及其致病作用研究 [D]. 成都:四川农业大学, 2009.Zhang X H. Study on the microorganism carried by Bursaphelenehus mucronatus and their pathogenicity [D]. Chengdu: Sichuan Agricultural University, 2009.
[23] Delalibera J R I, Vasanthakumar A, Burwitz B J, et al. Composition of the bacterial community in the gut of the pine engraver, Ips pini(Say)(Coleoptera)colonizing red pine [J]. Symbiosis(Rehovot), 2007, 43(2): 97-104.
[24] Zhang X, Yuen G. Effects of culture fluids and preinduction of chitinase production on biocontrol of bipolaris leaf spot by Stenotrophomonas maltophilia C3[J]. Biological Control, 2000, 18(3):277-286.
[25] Hollak C, Van weely S, Van oers M, et al. Marked elevation of plasma chitotriosidase activity. A novel hallmark of Gaucher disease [J]. Journal of Clinical Investigation, 1994, 93(3): 1288.
[26] H?fte M, De Vos P. Plant pathogenic Pseudomonas species[C]//Plant-associated bacteria. Netherlands:Springer, 2006: 507-533.
[27] Torres A R, Araujo W L, Cursino L, et al. Diversity of endophytic enterobacteria associated with different host plants [J]. The Journal of Microbiology, 2008, 46(4): 373-379.

拟松材线虫不同致病力虫株体内细菌的种类差异

Endobacteria from different virulence Bursaphelenchus mucronatus

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