亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的工艺优化

doi:10.3969/j.issn.1000-2006.2015.04.023

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (04): 132-136.doi: 10.3969/j.issn.1000-2006.2015.04.023

亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的工艺优化

任继巍¹,周洁²,许茜茜²,李鑫²,郑兆娟²,欧阳嘉^2*

1.南京林业大学林学院,江苏南京 210037;
2.南京林业大学化学工程学院,江苏南京 210037

出版日期:2015-07-31 发布日期:2015-07-31
基金资助:
收稿日期:2014-11-24 修回日期:2015-02-28基金项目:“十二五”国家科技支撑计划(2012BAD32B06); 教育部新世纪优秀人才支持计划(NCET-11-0988); 江苏省杰出青年基金项目(BK2012038); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:任继巍,硕士生。*通信作者:欧阳嘉,教授。E-mail:hgouyj@njfu.edu.cn。引文格式:任继巍,周洁,许茜茜,等. 亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的工艺优化[J]. 南京林业大学学报:自然科

Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation

REN Jiwei¹, ZHOU Jie², XU Qianqian², LI Xin², ZHENG Zhaojuan², OUYANG Jia^2*

1. College of Forestry, Nanjing Forestry University, Nanjing 210037, China;
2. College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China

Online:2015-07-31 Published:2015-07-31

摘要/Abstract

摘要： 亚硫酸盐蔗渣浆是一种较易水解的预处理纤维原料,经酶解可获得较高的葡萄糖得率和总糖得率。笔者以亚硫酸盐蔗渣浆为原料,利用树干毕赤酵母同步糖化发酵法生产乙醇,研究了其生产工艺,分析了温度、pH、酵母接种量、底物质量浓度和纤维素酶用量对亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的影响。结果表明,利用亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的优化条件为:底物质量浓度75 g/L,纤维素酶15 μmol/(min·g),酵母接种量0.13 g/L,温度36 ℃,pH 4.8。在此条件下亚硫酸盐蔗渣浆经树干毕赤酵母同步糖化发酵72 h,乙醇质量浓度达到17.89 g/L,为理论得率的55.99%。

Abstract: Bagasse sulfite pulp is a kind of pretreated cellulosic material, which can be easily hydrolyzed, and high glucose and total sugar yield can be obtained after enzymatic hydrolysis. In this study, the preparation of ethanol from bagasse sulfite pulp(BSP)was investigated using Pichia stipitis by simultaneous saccharification and fermentation(SSF). Effects of temperature, pH, inoculum size, substrate mass concentration and cellulase dosage on ethanol production were investigated. The results confirmed that ethanol could be produced from BSP using P. stipitis by SSF, and the optimal conditions were as follows: 36 ℃, pH 4.8, 0.13 g/L of inoculum size, 75 g/L of substrate mass concentration, 15 μmol/(min·g)cellulose of cellulase dosage. Under the optimal conditions, 17.89 g/L of ethanol mass concentration were produced in 72 h with 55.99% of theoretical yield. The optimized process of this study could provide technical references for ethanol production from BSP by SSF.

中图分类号:

TS713

任继巍,周洁,许茜茜,等. 亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的工艺优化[J]. 南京林业大学学报（自然科学版）, 2015, 58(04): 132-136.

REN Jiwei, ZHOU Jie, XU Qianqian, LI Xin, ZHENG Zhaojuan, OUYANG Jia. Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(04): 132-136.DOI: 10.3969/j.issn.1000-2006.2015.04.023.

参考文献

[1] 张强,戈兴炜,Anders Thygesen,等. 利用树干毕赤酵母发酵玉米秸秆制备燃料酒精[J]. 化工进展,2010,29(12):2270-2273.Zhang Q,Ge X W,Anders Thygesen,et al. Fuel ethanol production from corn stover by Pichia stipitis[J]. Chemical Industry and Engineering Process,2010,29(12):2270-2273.
[2] 朱均均,陈尚研,勇强,等. 玉米秸秆生物炼制燃料乙醇的研究[J]. 林产化学与工业,2011,31(6):35-40.Zhu J J,Chen S Y,Yong Q,et al. Biorefinery of corn stover for fuel ethanol[J]. Chemistry and Industry of Forest Products,2011,31(6):35-40.
[3] Menon V,Rao M. Trends in bioconversion of lignocellulose:Biofuels,platform chemicals & biorefinery concept[J]. Progress in Energy and Combustion Science,2012,38(4):522-550.
[4] Yu H,Zhang M,Ouyang J,et al. Comparative study on four chemical pretreatment methods for an efficient saccharification of corn stover[J]. Energy & Fuels,2014,28(7):4282-4287.
[5] 刘保田,蔡聪,冯萌,等. 亚硫酸盐蔗渣浆酶解工艺及外源物辅助作用的研究[J]. 林产化学与工业,2012,32(6):28-32.Liu B T,Cai C,Feng M,et al. The study about enzymatic hydrolysis of bagasse sulfite pulp and the assistant effect of additives[J]. Chemistry and Industry of Forest Products,2012,32(6):28-32.
[6] Adney B, Baker J. Biomass analysis technology team laboratory analysis procedure[R]. Golden,co,USA:National Renewable Energy Laboratory(NREL),1996.
[7] Podkaminer K K,Kenealy W R,Herring C D, et al. Ethanol and anaerobic conditions reversibly inhibit commercial cellulase activity in thermophilic simultaneous sacccharification and fermentation(tSSF)[J]. Biotechnology for Biofuels,2012,5:43.
[8] 金慧,刘荣厚. 温度对固定化酵母酒精分批发酵的影响及动力学模型[J]. 农业工程学报,2009,25(11):258-262.Jin H,Liu R H. Kinetic models and effects of temperature on ethanol production in batch fermentation by Saccharomyces cerevisiae strain[J]. Transaction of the CSAE,2009,25(11):258-262.
[9] Amillastre E,Aceves-Lara C-A,Uribelarrea J-L,et al. Dynamic model of temperature impact on cell viability and major product formation during fed-batch and continuous ethanolic fermentation in Saccharomyces cerevisiae[J]. Bioresource Technology,2012,117:242-250.
[10] 朱均均,勇强,徐勇,等. 树干毕赤酵母NLP31发酵产乙醇的研究[J]. 生物加工过程,2010,8(1):1-5.Zhu J J,Yong Q,Xu Y,et al. Fermentation production of ethanol by Pichia stipitis NLP31[J]. Chinese Journal of Bioprocess Engineering,2010,8(1):1-5.
[11] 岳昌海,朱许慧,侯文贵,等. 甘薯渣酒精发酵条件优化[J]. 化学工业与工程,2011,28(2):49-54.Yue C H,Zhu X H,Hou W G,et al. Optimization of fermentation conditions for raw starch residue of potatoes[J]. Chemical Industry and Engineering,2011,28(2):49-54.
[12] 罗鹏,刘忠,杨传民,等. 蒸汽爆破麦草同步糖化发酵转化乙醇的研究[J]. 化学工程,2007,35(12):42-45.Luo P,Liu Z,Yang C M,et al. Study of simutanoues saccharification and fermentation for steam exploded wheat straw to ethanol[J]. Chemical Engineering,2007,35(12):42-45.
[13] 张亚云,丁长河,李里特,等. 树干毕赤酵母发酵木糖生产燃料乙醇[J]. 酿酒,2009,36(1):23-26.Zhang Y Y,Ding C H,Li L T,et al. Fermentation of D-xylose for ethanol production by Pichia stipitis[J]. Liquor Making,2009,36(1):23-26.
[14] Matsushika A, Sawayama S. Characterization of a recombinant flocculent Saccharomyces cerevisiae strain that co-Ferments glucose and xylose:II. influence of pH and acetic acid on ethanol production [J]. Applied Biochemistry Biotechnology,2012,168(8):2094-2104.
[15] 季更生,勇强,余世袁. 发酵抑制物对树干毕赤酵母戊糖发酵的影响[J]. 南京林业大学学报:自然科学版,2007,31(6):133-135.Ji G S,Yong Q,Yu S Y. Effects of restrainers on fermentation of pentose by Pichia stipitis[J]. Journal of Nanjing Forestry University:Natural Sciences Edition,2007,31(6):133-135.
[16] 朱英波,史凤玉,武云鹏,等. 甘薯淀粉同步糖化发酵生产燃料乙醇的研究[J]. 江苏农业科学,2012,40(5):246-249.
[17] 彭林才,陈元彩,付时雨,等. 造纸污泥同步糖化发酵生产燃料乙醇[J]. 华南理工大学学报:自然科学版,2010,38(8):34-39.Peng L C,Chen Y C,Fu S Y,et al. Production of fuel ethanol from paper sludge via simutaneous saccharification and fermentation[J]. Journal of South China University of Technology:Natural Science Edition,2010,38(8):34-39.
[18] Zhang M,Wang F,Su R X,et al. Ethanol production from high dry matter corncob using fed-batch simultaneous saccharification and fermentation after combined pretreatment[J]. Bioresource Technology, 2010,101(13):4959-4964.
[19] Ouyang J,Liu B T,Zhang M,et al. Enzymatic hydrolysis, adsorption, and recycling during hydrolysis of bagasse sulfite pulp[J]. Bioresource Technology,2013,146:288-293.
[20] Tomás-Pej E,García-Aparicio M, Negro M J,et al. Effect of different cellulase dosages on cell viability and ethanol production by Kluyveromyces marxianus in SSF processes[J]. Bioresource Technology,2009,100(2):890-895.

亚硫酸盐蔗渣浆同步糖化发酵法产乙醇的工艺优化

Optimization of ethanol production from bagasse sulfite pulp by simultaneous saccharification and fermentation

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