稀硫酸水解预处理对麦草制浆备料废渣酶水解糖化的影响

吕佳青; 毕衍金; 严希希; 金永灿

doi:10.3969/j.issn.1000-2006.2015.04.024

吕佳青,毕衍金,严希希,金永灿

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (04) : 137-142.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (04) : 137-142. DOI: 10.3969/j.issn.1000-2006.2015.04.024

研究论文

稀硫酸水解预处理对麦草制浆备料废渣酶水解糖化的影响

吕佳青¹,毕衍金²,严希希¹,金永灿^1*

作者信息 +

Effect of dilute acid hydrolysis pretreatment on the enzymatic saccharification of wheat straw handling residue

LYU Jiaqing¹, BI Yanjin², YAN Xixi¹, JIN Yongcan^1*

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文章历史 +

摘要

以制浆厂麦草备料废渣为原料,探讨了稀硫酸水解预处理对其主要化学成分及酶水解糖化效率的影响。结果表明:麦草备料废渣经170 ℃自水解预处理后,酶水解效率较低; 预处理中加入不同量的稀硫酸水解有利于促进麦草备料废渣的酶水解糖化。未经洗涤直接磨浆得到的预处理浆料的酶水解总糖得率高于经水洗后的浆料。预处理后物料不经洗涤直接酶水解的总糖转化率随预处理酸用量的增加呈先升后降的趋势,经稀硫酸水解(H₂SO₄质量分数0.32%,170 ℃)和PFI磨浆(8 000 r)预处理得到的麦草备料废渣浆料,未经洗涤在酶用量20 μmol/(min·g)下酶水解,其葡聚糖和木聚糖转化率分别为71.9%和73.9%,总糖转化率达到最大值(近70%)。

Abstract

The wheat straw handling residue produced in pulp mills are difficult to be utilized efficiently and economically due to the complicated composition and large quantities. However, as a cheap lignocellulosic material, the utilization of the residue to produce fuel ethanol offers a great potential for reducing solid waste emission and producing great economic benefits. The effects of dilute acid hydrolysis pretreatment on chemical composition and enzymatic saccharification of wheat straw handling residue were investigated. The results showed that the sugar conversion of enzymatic saccharification was low after auto-hydrolysis pretreatment at 170 ℃. The addition of dilute sulfuric acid in the pretreatment was beneficial to enhance the enzymatic saccharification. The sugar conversion of unwashed pretreated wheat straw handling residue was higher than that of washed one. The total sugar conversion of unwashed pretreated materials increased with the increase dosage of sulfuric acid, and then it decreased with the continuous increasing charge. The glucan and xylan conversion reached up to 71.9% and 73.9%, respectively, and the total sugar conversion reached the maximum(closed to 70%), for the substrate with pre-hydrolysis(0.32% H₂SO₄, 170 ℃)and PFI refining(8 000 revolutions), hydrolyzed at a cellulase loading of 20 μmol/(min·g).

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吕佳青,毕衍金,严希希,金永灿. 稀硫酸水解预处理对麦草制浆备料废渣酶水解糖化的影响[J]. 南京林业大学学报（自然科学版）. 2015, 39(04): 137-142 https://doi.org/10.3969/j.issn.1000-2006.2015.04.024

LYU Jiaqing, BI Yanjin, YAN Xixi, JIN Yongcan. Effect of dilute acid hydrolysis pretreatment on the enzymatic saccharification of wheat straw handling residue[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(04): 137-142 https://doi.org/10.3969/j.issn.1000-2006.2015.04.024

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基金

收稿日期:2014-10-06 修回日期:2015-01-24基金项目:国家自然科学基金项目(31370571); 高等学校博士学科点专项科研基金(20133204110006); 江苏省基础研究计划(BK20141473); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:吕佳青,硕士生。*通信作者:金永灿,教授,博士。E-mail: jinyongcan@njfu.edu.cn。引文格式:吕佳青,毕衍金,严希希,等. 稀硫酸水解预处理对麦草制浆备料废渣酶水解糖化的影响[J]. 南京林业大