化学诱变绛红褐链霉菌对农药的耐药性

成丹; 朱天辉

doi:10.3969/j.issn.1000-2006.2015.05.005

化学诱变绛红褐链霉菌对农药的耐药性

成丹,朱天辉

南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (05) : 27-32.

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南京林业大学学报（自然科学版） ›› 2015, Vol. 39 ›› Issue (05) : 27-32. DOI: 10.3969/j.issn.1000-2006.2015.05.005

专题报道

化学诱变绛红褐链霉菌对农药的耐药性

成丹,朱天辉^*

作者信息 +

Pesticide resistance of Streptomyces purpeofuscus after chemical mutation

CHENG Dan,ZHU Tianhui^*

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文章历史 +

摘要

为筛选出具有耐药性的绛红褐链霉菌菌株,选用甲基磺酸乙酯(EMS)、叠氮化钠(NaN₃)、5-溴尿嘧啶(5-BU)3种化学诱变剂对生防菌绛红褐链霉YSSPG3进行化学诱变,再用多菌灵、百菌清对突变菌株进行药剂驯化,筛选出了4种能耐3 500 mg/L多菌灵的突变菌株,EC₅₀值在3 202.58～3 722.20 mg/L。其中耐药性最好的是经叠氮化钠诱变剂浓度为8 mmol/L诱变后的突变菌株,其EC₅₀值高达3 722.20 mg/L,遗传稳定性良好,经转接10代,每代的EC₅₀值没有较大的波动,基本趋于平稳。5种能耐1 500 mg/L百菌清的突变菌株,EC₅₀值在1 301.97～1 960.20 mg/L。其中耐药性最好的是经叠氮化钠诱变剂浓度为8 mmol/L诱变后的突变菌株,其EC₅₀值高达1 960.20 mg/L,且遗传稳定性较好。

Abstract

To select the drug-resistant strain, the Streptomyces purpeofuscus YSSPG3 was treated by chemical mutagen(Ethyl methane sulfonate,Sodium azide and 5-bromouracil)with different concentration,and then these mutation strains were screened out through the carbendazim and chlorothalonil.The drug resistant concentrations of four mutant strains tolerant to carbendazim were 3 500 mg/L,with EC₅₀ value ranging from 3 202.58 to 3 722.20 mg/L, while the drug-resistance of mutation strains induced by Sodium azide in the concentration of 8 mmol/L were the strongest with EC₅₀ value of 3 722.20 mg/L. It was also found that the resistance of resistant strains to carbendazim was stably inherited after continuous subculturing for 10 times. Meanwhile, the resistance tochlorothalonil of five mutation strains of Streptomyces purpeofuscus YSSPG3 could grow well when chlorothalonil concentration was 1 500 mg/L and the EC₅₀ value of these strains tested to chlorothalonil ranged from 1 301.97-1 960.20 mg/L. These results of drug-resistance experiment showed that the mutant strains which were treated by Sodium azide(8 mmol/L)presented the best resistance to chlorothalonil, with the EC₅₀ value reaching 1 960.20 mg/L.Under successive transfer culture, their drug-resistance heredity was also very stable.

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成丹,朱天辉. 化学诱变绛红褐链霉菌对农药的耐药性[J]. 南京林业大学学报（自然科学版）. 2015, 39(05): 27-32 https://doi.org/10.3969/j.issn.1000-2006.2015.05.005

CHENG Dan,ZHU Tianhui. Pesticide resistance of Streptomyces purpeofuscus after chemical mutation[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2015, 39(05): 27-32 https://doi.org/10.3969/j.issn.1000-2006.2015.05.005

中图分类号： S767.1

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基金

收稿日期:2014-09-14 修回日期:2014-12-09
基金项目:高等学校博士学科点专项科研基金项目(201300329701)
第一作者:成丹,硕士生。*通信作者:朱天辉,教授。E-mail: zhuth1227@126.com。
引文格式:成丹,朱天辉. 化学诱变绛红褐链霉菌对农药的耐药性[J]. 南京林业大学学报:自然科学版,2015,39(5):27-32.