蜡梅SAMT基因遗传转化及其功能分析

doi:10.3969/j.issn.1000-2006.2015.06.006

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南京林业大学学报（自然科学版） ›› 2015, Vol. 58 ›› Issue (06): 29-34.doi: 10.3969/j.issn.1000-2006.2015.06.006

蜡梅SAMT基因遗传转化及其功能分析

庞宏东^1,2,向林³,赵凯歌¹,李响¹,杨楠¹,陈龙清^1*

1.华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室,湖北武汉 430070;
2.湖北省林业科学研究院,湖北武汉 430075;
3.浙江省农业科学院园艺研究所,浙江杭州 310021

出版日期:2015-11-30 发布日期:2015-11-30
基金资助:
收稿日期:2014-06-08 修回日期:2014-10-08
基金项目:国家自然科学基金项目(30972019)
第一作者:庞宏东,硕士。*通信作者:陈龙清,教授。E-mail: chenlq0206@163.com。
引文格式:庞宏东,向林,赵凯歌,等. 蜡梅SAMT基因遗传转化及其功能分析[J]. 南京林业大学学报:自然科学版,2015,39(6):29-34.

Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox

PANG Hongdong^{1, 2}, XIANG Lin³, ZHAO Kaige¹, LI Xiang¹,YANG Nan¹, CHEN Longqing^1*

1.Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China;
2. Hubei Academy of Forestry, Wuhan 430075, China;
3 Institute of Horticulture, Zhejiang Academy of Agricultural Sciences,Hangzhou 310021, China

Online:2015-11-30 Published:2015-11-30

摘要/Abstract

摘要： 为研究蜡梅SAMT基因的调控功能,通过构建pBI121-CpSAMT植物双元表达载体,利用农杆菌介导法将其转入矮牵牛中,经PCR和RT-PCR对转化植株进行检测,同时采用顶空固相微萃取以及气相色谱-质谱技术(HS-SPME-GC-MS),对转基因矮牵牛鲜花进行花香成分分析。结果显示:9株转化苗均能扩增出目的条带,RT-PCR检测结果进一步证明,阳性植株均发生了正确转录; 转基因矮牵牛植株和未转基因对照植株在植株大小、叶片形态、花色、花瓣大小以及花期等方面均未发现有明显差异。鲜花芳香成分分析表明,转CpSAMT基因矮牵牛中苯甲醛和苯乙醇含量明显升高,并产生了香茅醇、乙酸香茅酯和乙酸苯乙酯等成分,但水杨酸甲酯和苯甲酸甲酯含量没有显著改变。

Abstract: In order to investigate the biological function of the Chimonanthus praecox SAMT gene, here we reported that the plant binary expression vector pBI121-CpSAMT was constructed and then the CpSAMT gene was introduced into petunia through Agrobacterium tumefaciens mediated transformation. PCR and RT-PCR was used to test the transgenic plants, and HS-SPME-GC-MS was employed to analyze the components of flower flavor. Results showed that nine independent transgenic lines obtained were positive. Further analysis showed that transgenic plants were indistinguishable from untransformed controls in terms of plant size, leaf morphology, flower color, petal size and flowering time. The analysis of HS-SPME-GC-MS showed that the content of benzaldehyde, phenylethanol, citronellol, citronellyl acetate and phenethyl acetate in flowers of transgenic CpSAMT plants was increased. However, the contents of methyl salicylate and methyl benzoate had not been significantly changed comparing with non-transgenic controls.

中图分类号:

Q78
S685

庞宏东,向林,赵凯歌,等. 蜡梅SAMT基因遗传转化及其功能分析[J]. 南京林业大学学报（自然科学版）, 2015, 58(06): 29-34.

PANG Hongdong, XIANG Lin, ZHAO Kaige, LI Xiang,YANG Nan, CHEN Longqing. Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox[J].Journal of Nanjing Forestry University (Natural Science Edition), 2015, 58(06): 29-34.DOI: 10.3969/j.issn.1000-2006.2015.06.006.

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蜡梅SAMT基因遗传转化及其功能分析

Genetic transformation and functional analysis of the SAMT gene in Chimonanthus praecox

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