为加快鹅耳枥在园林绿化中的应用,探究了鹅耳枥对盐胁迫的响应及其耐盐性,以2年生鹅耳枥幼苗为材料,研究6个盐分梯度(0、1、2、3、4、5 g/L)NaCl胁迫对鹅耳枥幼苗生长及生理生化特性的影响。结果表明:①盐胁迫抑制了鹅耳枥的生长,随着盐质量浓度的增大,苗高生长量、地径生长量、总干质量和相对含水量均逐渐下降; ②保护酶活性、可溶性糖含量和可溶性蛋白含量随着盐胁迫程度的增加先升高后降低; ③随着盐质量浓度的增大,叶片MDA含量、相对电导率和脯氨酸含量总体呈上升趋势,且在5 g/L盐质量浓度处理下均达到最大值。研究表明,鹅耳枥幼苗在低、中等盐含量(1～3 g/L)的环境中能够正常生长,而4～5 g/L盐胁迫时对其生长会造成损害。因此,鹅耳枥具有一定的耐盐能力,可在园林绿化中推广使用。
Abstract
To explore the responses of Carpinus turczaniowii to salt stress and its salt tolerance, we studied the growth, physiological and biochemical changes of two year old C. turczaninowii seedlings stressed with six gradients of NaCl concentration(0,1,2,3,4,5 g/L). The results showed: ① The growth of C. turczaninowii was inhibited by salt stress, because the height growth,diameter growth,dry weight and relative water content of seedlings decreased with the increase of NaCl concentration. ②The protective enzymes(SOD and POD)activity, soluble sugar content and soluble protein content was increased first and then decreased; ③With the increase of salt stress, MDA content, relative conductivity and proline content increased, and reached a maximum under 5 g/L NaCl. We concluded that C. turczaninowii seedings can grow under low and moderate NaCl concentration(1-3 g/L), but its growth was inhibited under 4-5 g/L NaCl. It indicated that C. turczaninowii seedlings has certain ability of salt tolerance,and they can be used widely in the landscape.
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参考文献
[1] 钱燕萍,程龙霞,祝遵凌. 鹅耳枥属植物种子研究进展[J]. 北方园艺,2013(22):192-195.
Qian Y P, Cheng L X, Zhu Z L.Research progress on seeds of Carpinus L. seeds[J].Northern Horticulture,2013(22):192-195.
[2] 朱峰山. 鹅耳枥亦可做盆景[J]. 园林,2004(10):49.
[3] 邱智敏,王国英,袁继标. 天台鹅耳枥育苗试验初报[J]. 福建林业科技,2013,40(3):131-133,142.
Qiu Z M,Wang G Y,Yuan J B.Preliminary report on seeding cultivation of Carpinus tientaiensis[J].Journal of Fujian Forestry Science and Technology,2013,40(3):131-133,142.
[4] 陈珍,陈模舜,孙骏威,等.天台鹅耳枥的组织培养与快速繁殖[J]. 安徽农业大学学报,2013,40(6):1009-1012.
Chen Z,Chen M S,Sun J W,et al. Tissue culture and rapid propagation of Carpinus tientaiensis[J].Journal of Anhui Agricultural University,2013,40(6):1009-1012.
[5] 陈之端,邢树平,梁汉兴,等.鹅耳枥和虎榛子(桦木科)雌性生殖器官的形态发生[J].植物学报, 2001, 43(11): 1110-1114.
Chen Z D, Xing S P, Liang H X, et al. Morphogenesis of female reproductive organs in Carpinus turczaninowii and Ostryopsis davidiana(Betulaceae)[J]. Acta Botanica Sinica, 2001, 43(11): 1110-1114.
[6] 祝遵凌,金建邦.鹅耳枥属植物研究进展[J].林业科技开发, 2013, 27(3): 10-14.
[7] Suzuki W.Germination traits and adaptive regeneration strategies of the three Carpinus species[J]. Journal of Forest Research,2000,5(3):181-185.
[8] Guo K.Germination,seeding survival and growth of two Carpinus species from a Chinese beech forest[J].Acta Phytoecologica Sinica,2000,24(4):385-390.
[9] 李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,1999.
[10] 陈建勋,王晓峰.植物生理学实验指导[M].广州:华南理工大学出版社,2005.
[11] Boscaiu M, Estrelles E, Soriano P, et al. Effects of salt stress on the reproductive biology of the halophyte Plantago crassifolia[J]. Biologia Plantarum, 2005, 49(1):141-143.
[12] 李胜强,冯志坚. 两种润楠属植物耐盐性研究[J].广东林业科技,2010,26(6):9-14.
Li S Q,Feng Z J.Study on salt tolerance of two species in Machilus[J].Guangdong Forestry Science and Technology,2010,26(6):9-14.
[13] 廖岩,彭友贵,陈桂珠.植物耐盐性机理研究进展[J].生态学报,2007,27(5):2077-2089.
Liao Y,Peng Y G,Chen G Z.Research advances in plant-tolerance mechanism[J].Acta Ecologica Sinica,2007,27(5):2077-2089.
[14] Ye C J,Zhao K F. Effects of adaptation to elevated salinity on some enzymes' salt-tolerance in vitro and physiological changes of eelgrass[J]. Acta Botanica Sinica,2002,44(7):788-794.
[15] 王利民,陈金林,梁珍海,等.盆栽滨梅幼苗对NaCl胁迫的响应[J].南京林业大学学报:自然科学版,2010,34(3):89-92.
Wang L M,Chen J L,Liang Z H,et al.Responses of beach plum(Prunus maritima)seedilngs to NaCl stress using pot culture experoments[J].Journal of Nanjing Forestry University:Natural Sciences Edition,2010,34(3):89-92.
[16] 李志萍,张文辉. NaCl胁迫对栓皮栎幼苗生长及其生理响应[J].西北植物学报,2013,33(8):1630-1637.
Li Z P,Zhang W H.Growth and physiological response of Quercus variabilis seedlins under NaCl stress[J].Acta Botanica Boreali-Occidentalia Sinica,2013,33(8):1630-1637.
[17] 刘爱荣,张远兵,钟泽华,等. 盐胁迫对彩叶草生长和渗透调节物质积累的影响[J]. 草业学报,2013,22(2):211-218.
Liu A R,Zhang Y B,Zhong Z H,et al.Effects of salt stress on the growth and osmotica accumulation of Coleus blumei[J]. Acta Prataculturae Sinica, 2013, 22(2):211-218.
[18] Munns R, Tester M. Mechanisms of salinity tolerance[J]. Annual Review of Plant Biology, 2008, 59:651-681.
[19] Rochaix J D. Regulation of photosynthetic electron transport[J]. Biochimica et Biophysica Acta(BBA)-Bioenergetics, 2011, 1807(3): 375-383.
[20] 周建,尤扬,于丽娜.盐胁迫对五角枫幼苗生长及生理特性的影响[J]. 广东农业科学,2012,39(8):53-56.
Zhou J,You Y,Yu L N.Effect of NaCl stress on germination and seedlings' physiological characteristices of Acer mono Maxim[J]. Guangdong Agricultural Sciences, 2012,39(8):53-56.
[21] 汪贵斌,曹福亮.盐胁迫对落羽杉生理及生长的影响[J].南京林业大学学报:自然科学版,2003,27(3):11-14.
Wang G B,Cao F L. Effects of salinity on growth and physiology of Bald cypress seedlings[J].Journal of Nanjing Forestry University:Natural Sciences Edition, 2003,27(3):11-14.
基金
收稿日期:2014-09-05 修回日期:2015-08-25
基金项目:江苏省工程技术研究中心建设项目(BM2013478); 江苏省科技支撑计划(BY2015006-01); 江苏省“六大人才高峰”项目(NY-029); 江苏省“青蓝工程”资助项目(2012)
第一作者:周琦,博士生。E-mial:806611458@qq.com。*通信作者:祝遵凌,教授,博士。E-mial:zhuzunling@aliyun.com
引文格式:周琦,祝遵凌,施曼. 盐胁迫对鹅耳枥生长及生理生化特性的影响[J]. 南京林业大学学报:自然科学版,2015,39(6):56-60.
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