一种云斑天牛气味结合蛋白的分离纯化

doi:10.3969/j.issn.1000-2006.2016.04.026

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (04): 164-170.doi: 10.3969/j.issn.1000-2006.2016.04.026

一种云斑天牛气味结合蛋白的分离纯化

卓志航¹,杨伟^1*,徐丹萍²,杨春平¹,杨桦¹,刘臣山³,谢婷婷¹

1. 四川农业大学林学院,四川温江 611130;
2. 四川农业大学食品学院,四川雅安 625014;
3. 成都农业科技职业学院农学院,四川温江 611130

出版日期:2016-08-18 发布日期:2016-08-18
基金资助:
收稿日期:2015-09-03 修回日期:2016-03-21
基金项目:国家自然科学基金项目(31270694); 四川农业大学“211”双程双支计划资助项目(00370101); 四川农业大学长江上游生态林业工程建设资助项目
第一作者:卓志航(450433697@qq.com)。*通信作者:杨伟(546756758@qq.com),教授。
引文格式:卓志航,杨伟,徐丹萍,等. 一种云斑天牛气味结合蛋白的分离纯化[J]. 南京林业大学学报(自然科学版),2016,40(4):164-170.

Isolation and purification of an odorant binding protein from Batocera lineolata Chevrolat

ZHUO Zhihang¹, YANG Wei^1*, XU Danping², YANG Chunping¹, YANG Hua¹, LIU Chenshan³, XIE Tingting¹

1. College of Forestry,Sichuan Agricultural University,Wenjiang 611130, China;
2. College of Food Science, Sichuan Agricultural University,Ya'an 625014, China;
3. College of Agriculture,Chengdu Vocational College of Agricultural Science and Technology,Wenjiang 611130, China

Online:2016-08-18 Published:2016-08-18

摘要/Abstract

摘要： 为揭示云斑天牛(Batocera lineolata Chevrolat)对气味分子的识别机制,通过荧光结合试验和葡聚糖凝胶G75(Sephadex G75)层析研究了云斑天牛对气味分子的识别情况,并对云斑天牛气味结合蛋白进行了分离纯化。结果表明,云斑天牛气味结合蛋白能与荧光探针N-苯基-1-萘胺(N-phenyl-1-naphthylamine, 1-NPN)产生荧光结合,云斑天牛触角部位的总蛋白提取液中存在pro. Ⅰ-Ⅻ(Protein Ⅰ-Ⅻ)12种大分子粗提蛋白,这12种大分子粗提蛋白经Sephadex G75纯化后,得到1种能与荧光探针1-NPN产生荧光结合的纯化蛋白pur-pro. I(purified protein I)。纯化蛋白pur-pro. I分子质量约为15.2 ku,对应为大分子粗提蛋白pro. Ⅻ(Protein Ⅻ)。鉴定认为,分离纯化蛋白pur-pro. I是云斑天牛的一种气味结合蛋白。

Abstract: The fluorescence binding assays and Sephadex G75 chromatography were conducted to investigate the recognition of odorants by Batocera lineolata Chevrolat. Additionally, one of the odorant binding proteins in B. lineolata was isolated and purified. The results showed that the odorant binding protein of B. lineolata could combine with fluorescent probe(N-phenyl-1-naphthylamine, 1-NPN). Besides, twelve kinds of macromolecular crude extract protein(pro. Ⅰ-Ⅻ, Protein Ⅰ-Ⅻ)were found in antenna of B. lineolata. When the 12 kinds of macromolecular crude extract protein were isolated and purified by Sephadex G75, a purified protein(pur-pro. I, purified protein I)was found, and it could also combine with fluorescent probe(N-phenyl-1-naphthylamine, 1-NPN). The relative molecular weight of pur-pro. I was approximately 15.2 ku, and it was pro. Ⅻ(Protein Ⅻ), when contrasted with all kinds of macromolecular crude extract protein. All above, it declared that the isolated and purified protein pur-pro. I was one of the odorant binding proteins in B. lineolata.

中图分类号:

卓志航,杨伟,徐丹萍,等. 一种云斑天牛气味结合蛋白的分离纯化[J]. 南京林业大学学报（自然科学版）, 2016, 40(04): 164-170.

ZHUO Zhihang, YANG Wei, XU Danping, YANG Chunping, YANG Hua, LIU Chenshan, XIE Tingting. Isolation and purification of an odorant binding protein from Batocera lineolata Chevrolat[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(04): 164-170.DOI: 10.3969/j.issn.1000-2006.2016.04.026.

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一种云斑天牛气味结合蛋白的分离纯化

Isolation and purification of an odorant binding protein from Batocera lineolata Chevrolat

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