细菌Hfq蛋白的结构、功能及作用机制

樊奔; 陈晟; 李昱龙

doi:10.3969/j.issn.1000-2006.2016.05.025

细菌Hfq蛋白的结构、功能及作用机制

樊奔,陈晟,李昱龙

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (05) : 155-162.

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (05) : 155-162. DOI: 10.3969/j.issn.1000-2006.2016.05.025

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细菌Hfq蛋白的结构、功能及作用机制

樊奔, 陈晟, 李昱龙

作者信息 +

Structure, function and mechanisms of bacterial protein Hfq

FAN Ben, CHEN Sheng, LI Yulong

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摘要

非编码小RNA(small RNA, sRNA)是细菌基因转录后调控的一个重要层次,也是近十年来原核生物研究领域的焦点之一。大多数sRNA的作用与Hfq蛋白密切相关,即Hfq可以促进sRNA与其靶标mRNA的互补配对,进而影响翻译的进行或者mRNA的稳定性。笔者对Hfq的结构、Hfq参与sRNA调节作用的机制、Hfq在多种细菌中的功能表型进行了综述。Hfq是一个保守的蛋白质,在很多细菌中广泛存在,并与真核生物中参与mRNA剪切与降解活动的Sm蛋白同源。在结构上,Hfq具有两个非等同的RNA结合面,可以结合并介导多个RNA分子的相互作用,其结构体现了和功能的高度统一性。目前,对Hfq的研究主要集中于革兰氏阴性细菌中,在革兰氏阳性细菌中,Hfq的功能尚不明晰; 此外,在许多重要的细菌中,Hfq影响功能表型的具体机制也不清楚。因此,今后有必要进一步精细研究Hfq的分子结构特征和功能特点,深入分析Hfq对细菌表型多样化的影响机制,探究Hfq影响靶标分子和功能表型的详尽机制。

Abstract

Bacterial small RNAs(sRNAs)have been one of the important research subjects in microbiology in last decade. They mainly act as a group of regulators controlling bacterial gene expression at post-transcriptional level. Most sRNAs regulate their mRNA targets involved by Hfq, which can facilitate base-paring of sRNA-mRNA and further affect mRNA stability or translation process. In this work the structure of Hfq, the molecular mechanisms of Hfq in sRNA regulation, and its influences detected in various bacterial species are reviewed. As a small chaperon protein ubiquitously present in many bacteria, the homohexamer form of Hfq possesses two unequivocal facets which can bind different types RNAs and promote their interaction. Such a structure provides a basis for its functional roles in mediating sRNA-mRNA interaction. While the hitherto studies on Hfq are mainly restricted in Gram-negative bacteria, its roles in some Gram-positive bacteria remain elusive. Moreover, the mechanisms underlying the impacts of Hfq on many important bacterial species have been still not clear. In future, it is necessary to further investigate the fine structure of Hfq with regard to its function as well as the detailed mechanism of the diverse bacterial phenotype affected by Hfq.

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樊奔,陈晟,李昱龙. 细菌Hfq蛋白的结构、功能及作用机制[J]. 南京林业大学学报（自然科学版）. 2016, 40(05): 155-162 https://doi.org/10.3969/j.issn.1000-2006.2016.05.025

FAN Ben, CHEN Sheng, LI Yulong. Structure, function and mechanisms of bacterial protein Hfq[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(05): 155-162 https://doi.org/10.3969/j.issn.1000-2006.2016.05.025

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基金

收稿日期:2016-01-29 修回日期:2016-04-29
基金项目:国家自然科学基金青年项目(31100081); 江苏省自然科学基金项目(BK20151514); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:樊奔(fanben@njfu.edu.cn),副教授,博士。
引文格式:樊奔, 陈晟, 李昱龙. 细菌Hfq蛋白的结构、功能及作用机制[J]. 南京林业大学学报(自然科学版),2016,40(5):155-162.