赤霉素诱导麻疯树雌雄花分化的研究

惠文凯; 杨舒贻; 陈涵斌; 李书琦; 彭昌操; 吴国江; 陈晓阳

doi:10.3969/j.issn.1000-2006.2016.06.027

赤霉素诱导麻疯树雌雄花分化的研究

惠文凯,杨舒贻,陈涵斌,李书琦,彭昌操,吴国江,陈晓阳

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (06) : 174-REF!.

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南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (06) : 174-REF!. DOI: 10.3969/j.issn.1000-2006.2016.06.027

研究简报

赤霉素诱导麻疯树雌雄花分化的研究

惠文凯¹,杨舒贻²,陈涵斌²,李书琦²,彭昌操²,吴国江³,陈晓阳^2*

作者信息 +

Female and male flower bud differentiation of Jatropha curcas L. induced by gibberellin

HUI Wenkai¹,YANG Shuyi²,CHEN Hanbin²,LI Shuqi²,PENG Changcao²,WU Guojiang³,CHEN Xiaoyang^2*

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摘要

为了探究外源喷施赤霉素(GA₃)对麻疯树花芽分化的影响,寻求赤霉素诱导雌花分化的适宜浓度,提高雌花比例,以3个麻疯树无性系为材料,用不同浓度的赤霉素喷施麻疯树花序芽,调查与花枝生长、花芽分化以及果实特性相关的14个性状,并进行数据分析。结果表明:赤霉素处理后,花序中花枝并未增多,雌雄花数量以及雌雄花比例均高于对照,且40 mg/L的赤霉素处理效果最佳,可显著促进雌花发育; 单花序果实数量增加,单果均质量、出籽率、百粒质量和种子含油量均显著低于对照; 在试验范围内,随着赤霉素浓度的提高,麻疯树花枝逐渐枯萎,1 200 mg/L赤霉素处理后的花枝全部枯萎,无果实采收。赤霉素的处理效果比较稳定,40 mg/L赤霉素可显著促进雌花发育。

Abstract

The purpose of this study was to study the effects of exogenous GA₃ on flower bud differentiation of Jatropha curcas and screen the optimum concentration of gibberellin to increase the ratio of female flowers. We used three clones of J. curcas as trial materials, investigated and analyzed 14 flowers after spraying different concentrations of GA₃ to inflorescence buds. The GA₃ could promote the development of female flower, all the male and female flowers enlarged and the total number and proportion of female flowers were higher than that of the control, but inflorescence branches did not increase. The effect of 40 mg/L GA₃ was optimum for the development of female flowers. However, average fruits per inflorescence, fruit weight, seed rate, 100-seeds weight and the seed oil content were significantly lower than that of the control after treatment. In this trial, with the increasing of GA₃ concentration, the inflorescence branch would be withered, until 1 200 mg/L treatment, all flowers withered and no fruits were harvested. In conclusion, the effect of GA₃ was relatively stable, and 40 mg/L GA₃ was optimum for the development of female flowers.

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惠文凯,杨舒贻,陈涵斌,李书琦,彭昌操,吴国江,陈晓阳. 赤霉素诱导麻疯树雌雄花分化的研究[J]. 南京林业大学学报（自然科学版）. 2016, 40(06): 174-REF! https://doi.org/10.3969/j.issn.1000-2006.2016.06.027

HUI Wenkai,YANG Shuyi,CHEN Hanbin,LI Shuqi,PENG Changcao,WU Guojiang,CHEN Xiaoyang. Female and male flower bud differentiation of Jatropha curcas L. induced by gibberellin[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2016, 40(06): 174-REF! https://doi.org/10.3969/j.issn.1000-2006.2016.06.027

中图分类号： Q945.6

参考文献

[1] 马金双. 中国植物志[M]. 北京: 科学出版社, 1997: 150.
[2] 中国-联合国开发计划署. 小桐子生产技术[M]. 成都: 四川大学出版社, 2007:179.
[3] Ginwal H S, Rawat P S, Srivastava R L. Seed source variation in growth performance and oil yield of Jatropha curcas Linn in central India[J]. Silvae Genetica, 2006, 55(2): 92.
[4] 王磊,汤庚国,刘彤. 6-BA、GA3和NAA对换锦花开花特性及内源激素的影响[J]. 南京林业大学学报(自然科学版),2009,33(5): 131-134.Doi:10.3969/j.issn.1000-2006.2009.05.029. Wang L, Tang G G, Liu T. Effects of 6-BA, GA3 and NAA on flowering and endogenous hormone content of Lycoris sprenger[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2009,33(5): 131-134.
[5] 黄众,陈天华,王章荣,等. 植物生长调节剂对马尾松种子园植株雄球花成花的作用[J]. 南京林业大学学报,1999,23(3): 87-89. Doi:10.3969/j.issn.1000-2006.1999.03.021. Huang Z, Chen T H, Wang Z R, et al. The role of plant growth regulators in flowering of male strobil in masson pine[J]. Journal of Nanjing Forestry University, 1999,23(3): 87-89.
[6] 江玲,周燮,王章荣,等. 马尾松雌雄球花的形成与赤霉素和脱落酸及细胞分裂素的关系[J]. 南京林业大学学报,1998,22(3): 64-68. Doi:10.3969/j.jssn.1000-2006.1998.03.014. Jiang L, Zhou X, Wang Z R, et al, Relationship between the formation of maleor female strobili and the levels of GAs,ABA,CTKs in masson pine[J]. Journal of Nanjing Forestry University, 1998,22(3): 64-68.
[7] Goldberg-Moeller R, Shalom L, Shlizerman L, et al. Effects of gibberellin treatment during flowering induction period on global gene expression and the transcription of flowering-control genes in citrus buds[J]. Plant Science, 2013, 198: 46-57. Doi: 10.1016/j.plantsci.2012.09.012.
[8] Pan B, Xu Z. Benzyladenine treatment significantly increases the seed yield of the biofuel plant Jatropha curcas[J]. Journal of Palnt Growth Regulation, 2011, 30(2): 166-174. Doi: 10.1007/s00344-010-9179-3.
[9] Ghosh A, Chikara J, Chaudhary D R, et al. Paclobutrazol arrests vegetative growth and unveils unexpressed yieldpotential of Jatropha curcas[J]. Journal of Plant Growth Regulation, 2010, 29(3): 307-315. Doi: 10.1007/s00344-010-9137-0.
[10] 王秀荣,赵杨,丁贵杰. 乙烯利对麻疯树成花与结实的影响[J]. 贵州农业科学,2012(6): 43-45. Doi:10.3969/j.issn.1001-3601.2012.06.012. Wang X R, Zhao Y, Ding G J. Effects of ethephon on flowering and fruiting of Jatropha curcas[J]. Guizhou Agricultural Sciences,2012(06): 43-45.
[11] 张宗俭,李斌. 世界农药大全:植物生长调节剂卷[M]. 北京: 化学工业出版社,2011.
[12] 何永梅,黄安乐. 植物生长调节剂在油菜生产上的应用[J]. 农药市场信息,2011(28): 39. Doi:10.13378/j.cnki.pmn.2011.28.028. He Y M, Huang A L. Application of plant growth regulator to Brassica campestris[J]. Pesticide Market News, 2011(28): 39.
[13] 葛长军,闫良,徐丽荣,等. 赤霉素对黄瓜雌性系的诱雄效果及诱导性状间的相关性研究[J]. 湖南农业科学,2016(2): 5-8. Doi:10.16498/j.cnki.hnnykx.2016.02.002. Ge C J, Yan L, Xu L R, et al. Male flower-inducing effects of gibberellin on cucumber gynoecious line and correlation analysis of induced features[J]. Hunan Agriculture Sciences, 2016(2): 5-8.
[14] 霍晓兰,刘和,冀爱青. 植物生长调节剂在核桃上的应用[J]. 山西农业科学,2003(1): 34-39. Doi:10.3969/j.issn.1002-2481.2003.01.010. Huo X L, Liu H, Ji A Q. Application of plant growth regulator to walnut tree[J]. Journal of Shanxi Agricultural Sciences, 2003(1): 34-39.
[15] 吕守芳,闫爱玲,王贵禧. 植物生长调节剂对板栗生长、性别分化和结蓬的影响[J]. 林业科学研究,2003(5): 642-645. Doi:10.3321/j.issn:1001-1498.2003.05.019. Lu S F, Yan A L, Wang G X. Effect of plant growth regulators on growth, sex differentiation and fructification of Chinese chestnut[J]. Forest Research, 2003(5): 642-645.
[16] Makwana V, Shukla P, Robin P. GA application induces alteration in sex ratio and cell death in Jatropha curcas[J]. Plant Growth Regulation, 2010, 61(2): 121-125. Doi: 10.1007/s10725-010-9457-x.
[17] 皮雪静,潘帮珍,徐增富. 赤霉素诱导小桐子产生两性花[J]. 植物分类与资源学报,2013(1): 26-32.Doi: 10.7677 /ynzwyj201312063. Pi X J, Pan B Z, Xu Z F. Induction of bisexual flowers by gibberellin in monoecious biofuel plant Jatropha curcas(Euphorbiaceae)[J]. Plant Diversity and Resources, 2013(1): 26-32.
[18] 许新桥,王伟,褚建民. 毛乌素沙地长柄扁桃31个优良单株坚果核仁脂肪酸组成变异分析[J]. 林业科学,2015,51(7): 142-147. Doi: 10.11707 /j.1001-7488.20150716. Xu X Q, Wang W, Chu J M. Variation analysis on kernel oil content and its fatty acid composition in 31superior amygdalus pedunculata individuals from Mu Us Desert[J]. Scientia Silvae Sinicae, 2015(7): 142-147.
[19] 吕兆林,李月琪,秦娇,等. 毛竹叶挥发油的提取方法[J]. 北京林业大学学报,2008,30(4): 135-140.Doi:10.3321/j.issn:1000-1522.2008.04.024. Lyu Z L, Li Y Q, Qin J, et al. Extraction techniques of volatile oil from Phyllostachys heterocyla cv. Pubescens leaves[J]. Journal of Beijing Forestry University, 2008,30(4): 135-140.
[20] Wu J, Liu Y, Tang L. A study on structural features in early flower development of Jatropha curcas L. and the classification of its inflorescences[J]. African Journal of Agricultural Research, 2011, 2(6): 275-284.
[21] Yu H, Ito T, Zhao Y, et al. Floral homeotic genes are targets of gibberellin signaling in flower development[J]. Proc Natl Acad Sci, 2004, 101(20): 7827-7832. Doi: 10.1073/pnas.0402377101.
[22] Mutasa-Gottgens E, Hedden P. Gibberellin as a factor in floral regulatory networks[J]. Journal of Experimental Botany, 2009, 60(7): 1979-1989. Doi: 10.1093/jxb/erp040.
[23] Hou X, Zhou J, Liu C, et al. Nuclear factor Y-mediated H3K27me3 demethylation of the SOC1 locus orchestrates flowering responses of Arabidopsis[J]. Nature Communications, 2014, 5:4601. Doi: 10.1038/ncomms5601.
[24] 杨国顺,刘昆玉,倪建军,等. 植物生长调节剂对板栗花芽性别分化及结果枝生长的影响[J]. 湖南农业大学学报(自然科学版),2001,27(6): 467-468. Doi:10.3321/j.issn:1007-1032.2001.06.016. Yang G S, Liu K Y, Ni J J, et al. Effects of growth regulators on flower sex differentiation of Chinese chestnut[J]. Journal of Hunan Agricultural University(Natural Sciences), 2001,27(6): 467-468.
[25] Yamaguchi N, Winter C M, Wu M F, et al. Gibberellin acts positively then negatively to control onset of flower formation in Arabidopsis[J]. Science, 2014, 344(6184): 638-641. Doi: 10.1126/science.1250498.
[26] Blazquez M A, Green R, Nilsson O, et al. Gibberellins promote flowering of Arabidopsis by activating the LEAFY promoter[J]. Plant Cell, 1998, 10(5): 791-800. Doi: 10.2307/3870665.
[27] Galvao V C, Horrer D, Kuettner F, et al. Spatial control of flowering by DELLA proteins in Arabidopsis thaliana[J]. Development, 2012, 139(21): 4072-4082. Doi: 10.1242/dev.080879.
[28] Moon J, Suh S S, Lee H, et al. The SOC1 MADS-box gene integrates vernalization and gibberellin signals for flowering in Arabidopsis[J]. Plant Journal, 2003, 35(5): 613-623. Doi: 10.1046/j.1365-313X.2003.01833.x.
[29] Eriksson S, Bohlenius H, Moritz T, et al. GA(4)is the active gibberellin in the regulation of LEAFY transcription and Arabidopsis floral initiation[J]. Plant Cell, 2006, 18(9): 2172-2181. Doi: 10.1105/tpc.106.042317.
[30] 马文波. 不同穗重类型水稻品种源、流、库特性的研究[D]. 成都:四川农业大学, 2002. Ma W B. The characteristics of Source-Transmit-Sink of the different panicle weight types of rice[D]. Chengdu:Sichuan Agricultural University, 2002.
[31] 王敏. 关于水稻颖果发育的研究[D]. 扬州:扬州大学, 2011. Wang M. Caryopsis Development of Rice[D]. Yangzhou: Yangzhou University, 2011.
[32] Bethke P C, Lonsdale J E, Fath A, et al. Hormonally regulated programmed cell death in barley aleurone cells[J]. Plant Cell, 1999, 11(6): 1033-1045. Doi: 10.1046/j.1365-313X.2003.01833.x.
[33] Bethke P C, Jones R L. Cell death of barley aleurone protoplasts is mediated by reactive oxygen species[J]. Plant Journal, 2001, 25(1): 19-29. Doi: 10.2307/3870796.
[34] 刘方炎,李昆,张春华,等. 麻疯树开花数量大小及其生态适应性[J]. 南京林业大学学报(自然科学版),2010, 34(6): 28-32. Doi:10.3969/j.jssn.1000-2006.2010.06.007. Liu F Y, Li K, Zhang C H, et al. Study on the floral quantity size and ecological adapt ability of Jatropha curcas[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2010, 34(6): 28-32.

基金

基金项目:国家自然科学基金项目(30972388); 广东省自然科学基金团队项目(9351064201000002)
第一作者:惠文凯(xwk168@126.com)。
*通信作者:陈晓阳(xychen@scau.edu.cn),教授。
引文格式:惠文凯,杨舒贻,陈涵斌,等. 赤霉素诱导麻疯树雌雄花分化的研究[J]. 南京林业大学学报(自然科学版),2016,40(6):174-180.