柳杉种子合子胚的愈伤组织诱导及植株再生

doi:10.3969/j.issn.1000-2006.2016.06.028

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

南京林业大学学报（自然科学版） ›› 2016, Vol. 40 ›› Issue (06): 181-186.doi: 10.3969/j.issn.1000-2006.2016.06.028

柳杉种子合子胚的愈伤组织诱导及植株再生

董京,施季森,徐进^*

南方现代林业协同创新中心,南京林业大学林学院,江苏南京 210037

出版日期:2016-12-18 发布日期:2016-12-18
基金资助:
基金项目:国家林业公益性行业科研专项项目(201304104); 江苏省“六大人才高峰”项目(2014-NY-013); 江苏高校优势学科建设工程资助项目(PARD); 福建省林木种苗科技攻关四期项目(20130121)
第一作者:董京(327497637@qq.com)。
*通信作者:徐进(xjinhsh@njfu.edu.cn),教授。
引文格式:董京,施季森,徐进. 柳杉种子合子胚的愈伤组织诱导及植株再生[J]. 南京林业大学学报(自然科学版),2016,40(6):181-186.

Callus induction and plant regeneration from zygotic embryos of Cryptomeria fortunei

DONG Jing, SHI Jisen, XU Jin^*

Co-Innovation Center for Sustainable Forestry in Southern China, College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2016-12-18 Published:2016-12-18

摘要/Abstract

摘要： 为探索柳杉种子合子胚的愈伤组织诱导技术,选取柳杉4个家系,采集其尚未完全成熟的种子进行愈伤组织的诱导,并经器官发生再生途径得到再生植株。利用6-BA、2,4-D等激素种类及其浓度的正交设计,研究各因素及其交互作用对柳杉种子合子胚愈伤组织诱导的影响。结果发现:柳杉4个家系的愈伤组织诱导量在8月中旬采种时呈明显上升,至8月底时各家系的子叶胚愈伤诱导率达到最高,其中57号家系愈伤诱导率最高,达92%。柳杉4个家系,在不同激素组合中愈伤诱导率存在着一定的差异。其中57号家系子叶胚在DCR+6-BA1.0 mg/L+2,4-D 1.0 mg/L培养时,愈伤组织诱导率达到最高,且诱导出来的愈伤组织状态为白色、干燥、无针状突起,结构紧密。愈伤组织在DCR培养基上成功获得再生植株。

Abstract: In this article,immature seeds from 4 families were selected to conduct callus induction and then regeneration plants were obtained by organogenesis in Cryptomeria fortunei by orthogonal design with different types of hormones and concentration. The effect caused by respective factors and interactions on callus induction from zygotic embryos of Cryptomeria fortunei were studied. The following were the results: ① The amount of callus induction from 4 families increased obviously collecting seeds in the mid August. By the late August, the callus induction rate from each zygotic embryos reached the highest, among which family NO. 57 family took the first place(92%). ② There were certain differences between the induction rate by using different hormone combinations. The highest induction rate of callus was that of family NO. 57 family which grew on DCR+6-BA 1.0 mg/L +2,4-D 1.0 mg/L culture medium,and the callus induced was white, dry and of highly dense form, with no needle. ③ Regeneration plants were successfully abtained from callus on the DCR basic medium.

中图分类号:

董京,施季森,徐进. 柳杉种子合子胚的愈伤组织诱导及植株再生[J]. 南京林业大学学报（自然科学版）, 2016, 40(06): 181-186.

DONG Jing, SHI Jisen, XU Jin. Callus induction and plant regeneration from zygotic embryos of Cryptomeria fortunei[J].Journal of Nanjing Forestry University (Natural Science Edition), 2016, 40(06): 181-186.DOI: 10.3969/j.issn.1000-2006.2016.06.028.

参考文献

[1] 中国科学院中国植物志编辑委员会. 中国植物志[M].北京:科学出版社, 1978:293-295.
[2] 刘洪谔. 柳杉选择育种效果评价[J]. 林业科学,1982, 18(3):237,244. Liu H E. Evaluation of effectiveness in selective breeding of Cryptomeria species [J]. Scientia Silvae Sinicae, 1982,18(3):237,244.
[3] 陈岳武,贾稝,康亦强,等. 福建柳杉小群体遗传变异的研究[J]. 南京林产工业学院学报,1982,6(3):76-83. Chen Y W, Jia P, Kang Y Q, et al. A study of the inheritance and variation of the small populations of Cryptomeria fortunei in Fujian Province [J]. Journal of Nanjing Technological College of Forest Products, 1982,6(3):76-83.
[4] 席梦利, 施季森. 杉木合子胚愈伤组织的诱导及植株再生[J]. 南京林业大学学报(自然科学版), 2006,30(2): 6-10. Doi:10.3969/j.issn.1000-2006.2006.02.002. Xi M L, Shi J S. Callus induction and plantlets regeneration from zygotic embryos of Cunninghamia lanceolata Hook[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2006, 30(2): 6-10.
[5] 陈剑勇. 杉木茎尖诱导愈伤组织及植株再生研究[J]. 西南林学院学报, 2009, 29(5): 37-41. Doi:10.3969/j.issn.2095-1914.2009.05.009. Chen J Y. Study on callus induction and plant regeneration by shoot tip of Cunninghamia lanceolate [J]. Journal of Southwest Forestry College, 2009, 29(5): 37-41.
[6] 高燕.马尾松体细胞胚胎发生系统建立[D].南京:南京林业大学,2010. Gao Y. Establishment of somatic embryogenesis system in Pinus massoniana [D]. Nanjing: Nanjing Forestry University, 2010.
[7] 黄翠,景丹龙,王玉兵,等.水杉愈伤组织诱导及植株再生[J]. 植物学报,2010,45(5): 604-608 Huang C, Jing D L, Wang Y B,et al. Callus induction and plant regeneration of a living fossil, Metasequoia glyptostroboides[J]. Chinese Bulletin of Botany, 2010,45(5): 604-608.
[8] Hakman I, Fowke L C, Von Arnold S, et al. The development of somatic embryos in tissue cultures initiated from immature embryos of Picea abies(Norway Spruce)[J]. Plant Science, 1985, 38(1): 53-59. Doi:10.1016/0168-9452(85)90079-2.
[9] Bornman C H. Possibilities and constraints in the regeneration of trees from cotyledonary needles of Picea abies in vitro[J]. Physiol Plant, 1983, 57(1): 5-16. Doi:10.1111/j.1399-3054.1983.tb00722.x.
[10] 张宇,卫志明,席梦利,等. 马尾松高效再生体系的建立 [J].分子细胞生物学报, 2006,39(3):271-274. Zhang Y, Wei Z M, Xi M L, et al. Efficient plant regeneration in vitro in Pinus massoniana [J]. Journal of Molecular Cell Biology, 2006,39(3):271-274.
[11] 杨金玲, 桂耀林, 郭仲琛. 云杉属树种的体细胞胚胎发生[J]. 植物学通报 1999,16(1):59-66. Doi:10.3969/j.issn.1674-3466.1999.01.008. Yang J L, Gui Y L, Guo Z C. Somatic embryo genesis of Picea species [J]. Chinese Bulletin of Botany, 1999,16(1):59-66.
[12] Tremblay F M. Somatic embryogenesis and plantlet regeneration from embryos isolated from stored seeds of Picea glauca [J]. Can J Bot, 1990, 68(2): 236-242. Doi:10.1139/b90-033.
[13] Lin X, Zhang W, Takechi K, et al. Stable genetic transformation of Larix gmelinii L. by particle bombardment of zygotic embryos[J]. Plant Cell Rep, 2005, 24(7): 418-425. Doi:10.1007/s00299-005-0955-7.
[14] 李际红,邢世岩,孟凡志,等. 白皮松成熟胚不定芽诱导分化技术的优化[J].山东农业大学学报(自然科学版),2009,40(4):641-646. Doi:10.3969/j.issn.1000-2324.2009.04.034. Li J H, Xing S Y, Meng F Z, et al. Improvement of in vitro rapid multiplication in muture embryo of Pinus bungeana [J]. Journal of Shandong Agricultural University(Natural Science Edition), 2009,40(4):641-646.
[15] Taniguchi T, Kurita M, Itahana N, et al. Somatic embryogenesis and plant regeneration from immature zygotic embryos of Hinoki cypress(Chamaecyparis obtusa Sieb. et Zucc.)[J]. Plant Cell Rep, 2004, 23(1-2): 26-31. Doi:10.1007/s00299-004-0803-1.
[16] Webb D T, Webster F, Flinn B S, et al. Factors influencing the induction of embryogenic and caulogenic callus from embryos of Picea glauca and P. engelmanii [J]. Canadian Journal of Forest Research, 1989, 19(10): 1303-1308. Doi:10.1139/x89-200.
[17] Go N E, Perez-Orozeo G D, Halos S C. In vitro response of embryos from different provenances of Pinus caribaea var. hondurensis Morelet[J]. Plant Cell Tiss Organ Cult, 1993, 32(1): 1-7. Doi:10.1007/bf00040109.
[18] Tuskan G A, Sargent W A, Rensema T, et al. Influence of plant growth regulators, basal media and carbohydrate levels on the in vitro development of Pinus ponderosa (Dougl. ex Law.)cotyledon explants[J]. Plant Cell Tiss Organ Cult, 1990, 20(1): 47-52. Doi:10.1007/bf00034756.
[19] 肖莉杰, 王丽艳, 闵丽, 等. 玉米成熟胚愈伤组织诱导及褐化控制研究[J]. 玉米科学, 2011, 19(4): 37-42. Xiao L J,Wang L Y, Min L, et al. Study on the inducing and browning control of callus initiated from maize mature embryos[J]. Journal of Maize Sciences, 2011, 19(4): 37-42.

柳杉种子合子胚的愈伤组织诱导及植株再生

Callus induction and plant regeneration from zygotic embryos of Cryptomeria fortunei

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

编辑推荐

Metrics

本文评价

作者

读者

审稿

[1]	罗建勋, 刘芙蓉, 宋鹏, 赖世会. 柳杉新品种‘福胖’[J]. 南京林业大学学报（自然科学版）, 2023, 47(1): 241-242.
[2]	骆鹏,曹玉婷,莫家兴,翁怀峰,施季森,徐进. 柳杉无性系指纹图谱的构建及遗传多样性分析[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 191-196.