解淀粉芽孢杆菌HZM9菌株发酵液的抑菌谱及稳定性测定

doi:10.3969/j.issn.1000-2006.201603053

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (03): 65-70.doi: 10.3969/j.issn.1000-2006.201603053

解淀粉芽孢杆菌HZM9菌株发酵液的抑菌谱及稳定性测定

胡忠亮,郑催云,田兴一,李警保,韩正敏,樊奔^*

南京林业大学林学院, 江苏南京 210037

出版日期:2017-06-18 发布日期:2017-06-18
基金资助:
收稿日期:2016-03-28 修回日期:2016-12-15
基金项目:国家自然科学基金青年项目(31100081); 江苏省自然科学基金项目(BK20151514); 江苏高校优势学科建设工程资助项目(PAPD)
第一作者:胡忠亮(15077876305@163.com)*通信作者:樊奔(fanben2000@gmail.com),副教授,博士。
引文格式:胡忠亮,郑催云,田兴一,等. 解淀粉芽孢杆菌HZM9菌株发酵液的抑菌谱及稳定性测定[J]. 南京林业大学学报(自然科学版),2017,41(3):65-70.

Determination of the inhibitory spectrum and stability of culture filtrate from Bacillus amyloliquefaciens HZM9

HU Zhongliang, ZHENG Cuiyun, TIAN Xingyi, LI Jingbao, HAN Zhengmin, FAN Ben^*

College of Forestry, Nanjing Forestry University, Nanjing 210037, China

Online:2017-06-18 Published:2017-06-18

摘要/Abstract

摘要： 【目的】将前期分离到的1株具有高效植物促生作用和生物防治作用的解淀粉芽孢杆菌HZM9开发为可以进行田间应用的菌剂。【方法】首先以10种土传病害病原菌为指示菌,用生长速率法测定了HZM9菌株发酵液对各病菌的抑制作用; 又以榉树枯萎尖孢镰刀菌(Fusarium oxysporum)为指示菌,测定了发酵液在不同条件下的稳定性。【结果】供试的10种土传病害病原菌经菌株HZM9发酵液处理后,菌丝生长均受到明显的抑制,特别是对尖孢镰刀菌和大豆菌核(Sclerotinia sclerotiorum)的抑菌率均达90%左右。菌株HZM9发酵液有很好的热稳定性,温度升至80 ℃后其抑菌作用变化十分微弱,抑菌率仍然保持在80%以上; 100 ℃处理30 min抑菌率会下降到66.55%,100 ℃处理120 min,抑菌率仍能达到58.78%; 在普通光照和紫外线照射后可以保持稳定的抑菌活性,在pH 5～9和蛋白酶的处理后抗菌活性也很稳定; 在4 ℃下可以持续储藏150 d。【结论】HZM9具有广谱抑菌活性和很好的稳定性,能够适用于土传病害的生物防治。

Abstract: 【Objective】Bacillus amyloliquefaciens HZM9 is a plant growth-promoting rhizobacterium with potent biocontrol activity, and its inhibitory spectrum and stability were studied to develop this strain as a bioagent to suppress soil-borne plant diseases. 【Method】The growth rate of 10 soil-borne plant pathogens was measured to test the inhibitory spectrum of the HZM9 culture filtrate. In addition, the growth rate of Fusarium oxysporum was tested to determine the stability of the HZM9 culture filtrate. 【Result】The results showed that the filtrate exhibited a broad spectrum against all 10 soil borne plant pathogens. In particular, Sclerotinia sclerotiorum and Fusarium oxysporum were strongly inhibited by the filtrate with an inhibitory rate of 90%. Antibacterial activity of the HZM9 filtrate had good thermal stability. When the temperature was higher than 80 ℃, the inhibitory rate of the strain HZM9 almost unchanged, and remained higher than 80%. At the temperature of 100 ℃ treated for 30 min, the inhibitory rate decreased to 66.55%, and for 30 min at 100 ℃, the inhibitory rate could still reach 58.78%.It was stable when exposed to a dose of light and ultraviolet rays. It was also stable at pH 5-9 and after proteases treatment. The filtrate could be stored with stable activity at 4 ℃ for 150 d.【Conclusion】 B. amyloliquefaciens HZM9 culture filtrate has a broad inhibitory spectrum and good stability, therefore, it could potentially be used in the biological control of soil-borne plant diseases.

中图分类号:

S763.15

胡忠亮,郑催云,田兴一,等. 解淀粉芽孢杆菌HZM9菌株发酵液的抑菌谱及稳定性测定[J]. 南京林业大学学报（自然科学版）, 2017, 41(03): 65-70.

HU Zhongliang, ZHENG Cuiyun, TIAN Xingyi, LI Jingbao, HAN Zhengmin, FAN Ben. Determination of the inhibitory spectrum and stability of culture filtrate from Bacillus amyloliquefaciens HZM9[J].Journal of Nanjing Forestry University (Natural Science Edition), 2017, 41(03): 65-70.DOI: 10.3969/j.issn.1000-2006.201603053.

参考文献

[1] 李洪连, 黄俊丽, 袁红霞. 有机改良剂在防治植物土传病害中的应用[J]. 植物病理学报, 2002, 32(4): 289-295. DOI:10.13926/j.cnki. apps. 2002. 04. 001. LI H L, HUANG J L, YUAN H X. Advances in control of plant soil-borne diseases by organic amendments[J]. Acta Phytopathologica Sinica, 2002, 32(4): 289-295.
[2] ZAKI K, MISAGHI I J, HEYDARI A, et al. Control of cotton seedling damping-off in the field by Burkholderia(Pseudomonas)cepacia[J]. Plant Disease, 1998, 82(3): 291-293. DOI: 10.1094/PDIS.1998.82.3.291.
[3] HEYDARI A, MISAGHI I J. Biocontrol activity of Burkholderia cepacia against Rhizoctonia solani in herbicide-treated soils[J]. Plant and Soil, 1998, 202(1): 109-116. DOI: 10.1023/A:1004370810139.
[4] 徐美娜, 王光华, 靳学慧. 土传病害生物防治研究进展[J]. 吉林农业科学, 2005, 30(2): 39-42. DOI:10.3969/j.issn.1003-8701.2005.02.012. XU M N, WANG G H, JIN X H. Progress in studies on biological control of soil mediated diseases[J]. Journal of Jilin Agricultural Sciences, 2005, 30(2): 39-42.
[5] 李世东, 缪作清, 高卫东. 我国农林园艺作物土传病害发生和防治现状及对策分析[J]. 中国生物防治学报, 2011, 24(7): 433-440. LI S D, MIAO Z Q, GAO W D. Challenges, opportunities and obligations in management of soilborne plant diseases in China[J]. Chinese Journal of Biological Control, 2011, 24(7): 433-440.
[6] 杨敏馨,寇涛,李悦,等.解淀粉芽孢杆菌ES-2对肉鸡屠宰性能和肉品质及肌肉抗氧化能力的影响[J].南京农业大学学报,2016,39(2):255-261.DOI:10.7685/jnau.201505040. YANG M X,KOU T,LI R, et al. Effect of Bacillus amyloliquefaciens ES-2 on carcass characteristics, meat quality and antioxidative status of broilers[J].Journal of Nanjing Agricultural University,2016,39(2):255-261.
[7] IDRIS E E, BOCHOW H, ROSS H, et al. Use of Bacillus subtilis as biocontrol agent. VI. Phytohormone-like action of culture filtrates prepared from plant growth-promoting Bacillus amyloliquefaciens FZB24, FZB42, FZB45 and Bacillus subtilis FZB37[J]. Journal of Plant Diseases and Protection, 2004,111(6): 583-597.
[8] CHEN X H, VATER J, PIEL J, et al. Structural and functional characterization of three polyketide synthase gene clusters in Bacillus amyloliquefaciens FZB42[J]. Joumal of Baeteriology, 2006, 188(11): 4024-4036. DOI: 10.1128/JB.00052-06.
[9] 方中达. 植病研究方法[M]. 北京: 中国农业出版社, 1998: 248-249.
[10] 葛平华, 马桂珍, 付泓润, 等. 海洋解淀粉芽孢杆菌GM-1菌株发酵液抗菌谱及稳定性测定[J]. 农药, 2012, 51(10): 730-732,741. GE P H, MA G Z, FU H R, et al. The inhibitory spectrum and stability determination of marine Bacillus amyloliquefaciens GM-1 Strain[J]. Agrochemicals, 2012, 51(10), 730-732,741.
[11] 王慧萍. 拮抗性芽孢杆菌F53抑制茄子黄萎病菌Verticillium dahliae的研究[D]. 南京: 南京师范大学, 2005. WANG H P. Study on antagonistic Bacillus F53 inhibiting Verticillium dahliae[D]. Nanjing: Nanjing Normal University, 2005.
[12] ROMANO A, VITULLO D, PIETRO A D, et al. Antifungal lipopeptides from Bacillus amyloliquefaciens strain BO7[J]. Journal of Natural Products, 2011, 74(2): 145-151. DOI: 10.1021/np100408y.
[13] 朱丽梅, 吴小芹, 胡飞杰, 等. 解淀粉芽孢杆菌JK-JS3杀线活性物质的分离与结构分析[J]. 林业科学, 2011, 47(7): 133-137.DOI: 10.11707/j.1001-7488.20110720. ZHU L M, WU X Q, HU F J, et al. Extraction and structure analysis of the nematicidal active substances produced by Bacillus amyloliquefaciens JK-JS3[J]. Scientia Silvae Sinicae, 2011, 47(7): 133-137.
[14] 朱丽梅, 吴小芹, 徐旭麟. 松材线虫拮抗细菌的筛选和鉴定[J].南京林业大学学报(自然科学版), 2008, 32(3): 91-94. DOI: 10.3969/j.issn.1000-2006.2008.03.022. ZHU L M, WU X Q, XU X L. The screening and identification of the bacterium with nematicidal activity to Bursaphelenchus xylophilus[J]. Journal of Nanjing Forestry University(Natural Sciences Edition), 2008, 32(3): 91-94.
[15] ARREBOLA E, SIVAKUMAR D, KORSTEN L. Effect of volatile compounds produced by Bacillus strains on postharvest decay in citrus[J]. Biological Control, 2010, 53(1): 122-128. DOI: 10.1016/j.biocontrol.2009.11.010.
[16] 葛平华, 马桂珍, 付泓润, 等. 油菜菌核病菌拮抗海洋细菌 GM-1 菌株的种类鉴定及抑菌作用研究[J]. 植物保护, 2013, 39(2): 50-56. DOI: 10.3969/j.issn.0529-1542.2013.02.010. GE P H, MA G Z, FU H R, et al. Identification and inhibitory effects of the marine bacterial strain GM-1 antagonistic to Sclerotinia sclerotiorum[J]. Plant Protection, 2013, 39(2): 50-56.
[17] CALDEIRA A T, FEIO S S, ARTEIRO J M S, et al. Bacillus amyloliquefaciens CCMI 1051 in vitro activity against wood contaminant fungi[J]. Annals of Microbiology, 2007, 57(1): 29-33. DOI: 10.1007/BF03175046.
[18] 权春善, 王军华, 徐洪涛, 等. 一株抗真菌解淀粉芽孢杆菌的分离鉴定及其发酵条件的初步研究[J]. 微生物学报, 2006, 46(1): 7-12. DOI: 10.3321/j.issn:0001-6209.2006.01.003. QUAN C S, WANG J H, XU H T, et al. Identification and characterization of a Bacillus amyloliquefaciens with high antifungal activity[J]. Acta Microbiologice Sinica, 2006, 46(1):7-12.

解淀粉芽孢杆菌HZM9菌株发酵液的抑菌谱及稳定性测定

Determination of the inhibitory spectrum and stability of culture filtrate from Bacillus amyloliquefaciens HZM9

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