增施铁对镉胁迫下柳树生长及光合生理性能的改善

doi:10.3969/j.issn.1000-2006.2017.02.010

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (02): 63-72.doi: 10.3969/j.issn.1000-2006.2017.02.010

增施铁对镉胁迫下柳树生长及光合生理性能的改善

戴前莉¹,李金花^1*,胡建军¹,卢孟柱¹,GIUSEPPE Nervo²

1.林木遗传育种国家重点实验室,国家林业局林木培育重点实验室,中国林业科学研究院林业研究所,北京 100091;
2.Unità di ricerca per le produzioni legnose fuori foresta, CRA-PLF,Casale Monferrato 15033,Italy

出版日期:2017-04-18 发布日期:2017-04-18
基金资助:
收稿日期:2016-02-16 修回日期:2016-12-01
基金项目:林业科技成果国家级推广项目([2015]43号)
第一作者:戴前莉(daiqianli126@126.com)。*通信作者:李金花(lijinh@caf.ac.cn),副研究员,博士。
引文格式:戴前莉,李金花,胡建军,等. 增施铁对镉胁迫下柳树生长及光合生理性能的改善 Symbol`@@ [J]. 南京林业大学学报(自然科学版),2017,41(2):63-72.

Improvement on growth and photosynthetic physiological performance of three willow clones or cultivar under Cd treatments and supplying Fe

DAI Qianli¹, LI Jinhua^1*, HU Jianjun¹, LU Mengzhu¹, GIUSEPPE Nervo²

1. State Key Laboratory of Tree Genetics and Breeding, Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China;
2. Unità di ricerca per le produzioni legnose fuori foresta, CRA-PLF, Casale Monferrato 15033, Italy

Online:2017-04-18 Published:2017-04-18

摘要/Abstract

摘要： 【目的】了解Cd胁迫及增施Fe对不同柳树生长及光合生理的影响,探讨增施Fe对降低柳树Cd毒害和提高Cd耐性以及对柳树Cd修复的Fe调控作用。【方法】以旱柳‘Levante’和蒿柳无性系79026及790260为材料,利用1/2 Hoagland营养液(含25 μmol/L EDTA-Na₂Fe)培养法,以不同Cd浓度(0、10、50、100 μmol/L)(不增施Fe)和上述Cd浓度下增施Fe(25 μmol/L)两组处理,培养42 d后测定不同柳树生长量(苗高和地径)、干物质量(根、茎、叶)、光合作用指标(叶绿素a、b含量、叶绿素a/b、净光合速率、气孔导度和胞间CO₂浓度)和Cd、Fe含量(根、茎、叶)。【结果】①随着Cd浓度增大,3个无性系/品种生长量、干物质量、叶绿素a、b含量、净光合速率、气孔导度和Fe含量(茎、叶)减少,叶绿素a/b、胞间CO₂浓度、Cd含量(根、茎、叶)增大,但根Fe含量在低中浓度Cd(10、50 μmol/L)时逐渐增大,在高浓度Cd(100 μmol/L)时减少。在相同Cd浓度胁迫下,3个无性系/品种Cd和Fe含量大小为根﹥叶﹥茎,旱柳根Cd含量大于蒿柳,但叶Cd含量小于蒿柳。②与低浓度Cd时增施Fe相比,在中高浓度Cd时增施Fe后,3个无性系/品种叶绿素a、b含量、旱柳‘Levante’和蒿柳79026生长量和干物质量(旱柳‘Levante’茎除外)、旱柳‘Levante’叶绿素a/b和叶片净光合速率均显著减小,而3个无性系/品种Cd含量增大,Fe含量减少。在高浓度Cd时增施Fe后旱柳‘Levante’和蒿柳790260叶Cd含量均小于中浓度Cd时增施Fe的,且旱柳Cd含量(根、茎、叶)和Fe含量(叶)均小于蒿柳。③增施Fe提高了Cd胁迫下柳树生长和干物质量、叶绿素a、b含量、气孔导度及胞间CO₂浓度,特别是在高浓度Cd时增施Fe后降低了3个无性系/品种根和叶Cd含量,增加了茎Cd含量,旱柳Cd含量(根、茎和叶)均小于蒿柳。【结论】Cd胁迫抑制了柳树生长和干物质积累以及叶绿素合成、光合作用,在中、高浓度Cd胁迫时,对旱柳生长和干物质量积累的抑制作用大于蒿柳; 柳树根的Cd和Fe积累能力大于茎和叶,蒿柳Cd积累能力大于旱柳。增施Fe提高了Cd胁迫下柳树生长量和干物质量以及叶绿素含量,不同程度地增加了Cd含量和Fe含量,一定程度上缓解了Cd胁迫对柳树光合作用的影响,且对旱柳的影响作用大于蒿柳,这证实了Cd胁迫下增施Fe改善Cd胁迫下柳树对生长和生理的适应性。

Abstract: 【Objective】 This paper investigated the effect of Cd concentrations with/without extra Fe on growth and photosynthetic physiology of different willow clones/cultivar is to explore the role of extra Fe on reducing Cd stress and improving Cd tolerance of willow and to shed more light on the regulation of extra Fe under Cd stress as its results for important practical significance. 【Method】 In greenhouse, cuttings of three willow clones/cultivar, Salix matsudana ‘Levante’, S. viminalis cl. 79026 and S. viminalis cl. 790260, were used for hydroponic culture in half of Hoagland nutrient solution(25 μmol/L EDTA-Na₂Fe)with Cd concentrations(0, 10, 50, 100 μmol/L). In another hydroponic group, extra Fe(25μmol/L EDTA-Na₂Fe)were supplied in solution with different Cd concentrations mentioned above. Growth(height and diameter), biomass(root, stem and leaf), photosynthesis indicators(chlorophyll a and b content, ration of chlorophyll a to b, net photosynthesis, stomatal conductance and intercellular CO₂ concentration), Cd and Fe content of root, stem, leaf were measured for plants of each clone/cultivar after 42 d culture. 【Result】 ① With the increasing of Cd concentrations in solution, growth, biomass, chlorophyll a and b content, net photosynthetic rate, stomatal conductance and Fe content of stem and leaf decreased, however, ratio of chlorophyll a to b, intercellular CO₂ concentration, Cd content of root, stem, leaf increased. Meanwhile, Fe content of root in three clones/cultivar decreased under low and middle Cd concentration(10, 50 μmol/L)and decreased under high Cd concentration(100 μmol/L). Under the same Cd concentration, Cd and Fe content of three clones/cultivar were root> leaf> stem. Cd content of root in S. matsudana was more than that of S. viminalis, while Cd content of leaf in S. viminalis were more than that of S. matsudana. ② In comparison with that under the low Cd concentration with extra Fe, chlorophyll a and b content of three clones/cultivar, growth and biomass of S. matsudana‘Levante‘ and S. viminalis cl. 79026 except that stem biomass of S. matsudana ‘Levante’, ratio of chlorophyll a to b, net photosynthetic rate of S. matsudana ‘Levante’ were significantly decreased, while Cd and Fe content of three clones/cultivar were increased and decreased under the middle and high Cd concentration with extra Fe. Under the high Cd concentrations with extra Fe, Cd content of leaf in S. matsudana ‘Levante’ and S. viminalis cl. 790260 were less than that under low and middle Cd concentration with extra Fe. And Cd content of root, stem, leaf and Fe content of leaf in S. matsudana were less than these of S. viminalis. ③ Compared with that in the culture without extra Fe, extra Fe in the culture improved growth, biomass, chlorophyll a and b content, stomatal conductance and intercellular CO₂ concentration of willow under Cd stress, especially decreased Cd content of root and leaf and increased Cd content of stem under the high Cd concentrations with extra Fe. In addition, Cd content of root, stem and leaf in S. matsudana’ were less than these in S. viminalis. 【Conclusion】Cd stress inhibited growth, dry biomass accumulation, chlorophyll synthesis and photosynthesis of willow. At middle and high Cd concentrations, inhibition on growth and dry biomass accumulation of S. matsudana was greater than that of S. viminalis. Cd and Fe accumulation capacity of root was greater than that of stem and leaf in willow, while Cd accumulation capacity of S. viminalis was greater than that of S. matsudana. Extra Fe with Cd stress increased growth, biomass, chlorophyll content, Cd and Fe content of willow. As the result, extra Fe alleviated Cd stress on chlorophyll synthesis, photosynthesis, etc. of willow while effect on S. matsudana was greater than that on S. viminalis. The results showed that extra Fe supplying could improve growth and physiological adaptation of willows under Cd stress.

中图分类号:

S718.43

戴前莉,李金花,胡建军,等. 增施铁对镉胁迫下柳树生长及光合生理性能的改善[J]. 南京林业大学学报（自然科学版）, 2017, 41(02): 63-72.

DAI Qianli, LI Jinhua, HU Jianjun, LU Mengzhu, GIUSEPPE Nervo. Improvement on growth and photosynthetic physiological performance of three willow clones or cultivar under Cd treatments and supplying Fe[J].Journal of Nanjing Forestry University (Natural Science Edition), 2017, 41(02): 63-72.DOI: 10.3969/j.issn.1000-2006.2017.02.010.

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增施铁对镉胁迫下柳树生长及光合生理性能的改善

Improvement on growth and photosynthetic physiological performance of three willow clones or cultivar under Cd treatments and supplying Fe

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[1]	张银凤, 蔡洪月, 彭金根, 刘学军, 谢利娟, 张华, 王艳梅. 深圳城市公园不同栽植环境对毛棉杜鹃生长的影响[J]. 南京林业大学学报（自然科学版）, 2023, 47(2): 197-204.
[2]	崔令军, 刘瑜霞, 林健, 石开明. 盐胁迫下丛枝菌根真菌对桢楠根系生长和激素的影响[J]. 南京林业大学学报（自然科学版）, 2020, 44(4): 119-124.
[3]	常青山,张利霞,王建章,王梓,徐少君,康璐,严俊驿,杨梦浩,赵一凡,刘杨. 干旱和复水对4个芍药品种生理指标的影响及品种抗旱性评价[J]. 南京林业大学学报（自然科学版）, 2018, 42(06): 44-50.
[4]	曾俊,孙慧珍. 超声发射特征归类识别木质部栓塞信息[J]. 南京林业大学学报（自然科学版）, 2018, 42(01): 89-97.
[5]	茹广欣,刘小囡,朱秀红,张龙冲,王鋆瑞,周霜晴. 泡桐黄化突变体生理特性分析[J]. 南京林业大学学报（自然科学版）, 2017, 41(04): 181-185.
[6]	刘龙云,吴彩娥,李婷婷,陈宗勇. 棕榈花苞营养成分分析[J]. 南京林业大学学报（自然科学版）, 2017, 41(03): 193-197.
[7]	王景燕,唐海龙,龚伟,胡文,芶国军. 水肥耦合对汉源花椒幼苗生长、养分吸收和肥料利用的影响[J]. 南京林业大学学报（自然科学版）, 2016, 40(03): 33-40.
[8]	甘小洪1,2,丁雨龙1. 毛竹茎竿纤维细胞发育过程中酸性磷酸酶的动态变化[J]. 南京林业大学学报（自然科学版）, 2006, 30(03): 13-18.
[9]	余观夏1,阮锡根1,封维忠1,黄敏仁2. 杨树无性系超冷现象分析[J]. 南京林业大学学报（自然科学版）, 2006, 30(02): 67-70.
[10]	徐锡增1,徐呈祥2. 硅对盐胁迫下枣树根尖离子微域分布的影响[J]. 南京林业大学学报（自然科学版）, 2006, 30(02): 85-88.
[11]	徐呈祥1,2,马艳萍2,徐锡增1,胡恒康2. 盐胁迫下外源硅调节金丝小枣根和叶片膜脂肪酸的研究[J]. 南京林业大学学报（自然科学版）, 2006, 30(02): 89-93.
[12]	宋立奕1,2,方升佐1. 水培青檀幼苗对NaCl胁迫的生理响应[J]. 南京林业大学学报（自然科学版）, 2006, 30(02): 94-98.
[13]	朱万泽1,吴永波2,薛建辉2. 贡嘎山地区黄背栎的光合特性[J]. 南京林业大学学报（自然科学版）, 2006, 30(01): 25-28.
[14]	万劲1,2,石雷1*,张金政1,汤庚国2. 盐胁迫对鸢尾叶片生理指标的影响[J]. 南京林业大学学报（自然科学版）, 2006, 30(01): 57-60.
[15]	倪建中,王伟,郁书君,贺漫媚,张继方,代色平. 不同种源木棉生长及光合特性研究[J]. 南京林业大学学报（自然科学版）, 2015, 39(06): 185-189.