PEG和GA3引发处理对老化毛竹种子理化特性的影响

蔡春菊; 范少辉; 曹帮华; 赵建诚; 刘凤

doi:10.3969/j.issn.1000-2006.201701021

PEG和GA₃引发处理对老化毛竹种子理化特性的影响

蔡春菊,范少辉,曹帮华,赵建诚,刘凤

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (02) : 40-46.

PDF(1486013 KB)

国家林草科技领军期刊
中国精品科技期刊
中国高校百佳科技期刊
江苏省新闻出版政府奖期刊奖
RCCSE林学权威期刊（A+）
CSCD核心期刊
Scopus数据库收录期刊
中文核心期刊
SCD核心期刊

作者加群：102861116

微信公众号：南京林业大学学报

高级检索

PDF(1486013 KB)

南京林业大学学报（自然科学版） ›› 2018, Vol. 42 ›› Issue (02) : 40-46. DOI: 10.3969/j.issn.1000-2006.201701021

研究论文

PEG和GA₃引发处理对老化毛竹种子理化特性的影响

蔡春菊¹,范少辉^1*,曹帮华²,赵建诚^1,3,刘凤^1,2

作者信息 +

Effects of PEG and GA₃ priming on the physiological and biochemical characteristics of aged moso bamboo(Phyllostachys edulis)seeds

CAI Chunju¹, FAN Shaohui^1*, CAO Banghua², ZHAO Jiancheng^1,3, LIU Feng^1,2

Author information +

文章历史 +

摘要

【目的】研究聚乙二醇(PEG)和赤霉素(GA₃)引发处理对不同老化程度毛竹种子生理生化的影响,探寻提高毛竹种子活力的最佳处理方法。【方法】采用高温高湿法(温度40 ℃、相对湿度95%)模拟种子自然老化过程,筛选出不同活力水平的毛竹种子; 然后采用PEG和GA₃进行引发处理,测定其生理生化指标。【结果】随着毛竹种子老化程度加深,种子浸出液相对电导率、可溶性糖和MDA含量增加,可溶性蛋白质含量降低,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)等保护酶活性降低,种子内源GA₃和吲哚乙酸(IAA)含量降低,脱落酸(ABA)含量升高,GA₃/ABA比值降低。而且,种子老化程度越深,种子活力提高越显著。与未引发处理相比,PEG和GA₃处理均显著降低毛竹种子浸出液相对电导率和MDA含量,降低膜脂过氧化水平,保护细胞膜的完整性,提高可溶性蛋白质含量,保护酶活性及GA₃和IAA含量,降低ABA含量,从而提高GA₃/ABA比值。【结论】适宜的PEG(质量分数为20%、20℃浸种24 h)和GA₃(质量浓度为500 mg/L、25℃浸种12 h)引发处理均能提高老化毛竹种子的活力水平,促进种子萌发,可为毛竹实生苗繁育提供参考和技术指导。

Abstract

【Objective】The viability of moso bamboo(Phyllostachys edulis)seeds often decreases quickly during the natural storage process, resulting in a short life of seeds, so it is important to enhance the vigor and germination rate of moso bamboo seeds for storage, production, and utilization. The objective of the present study was to investigate the effects of polyethylene glycol(PEG)and gibbereilin(GA₃)priming on the physiological and biochemical characteristics of aged moso bamboo seeds, and explore optional methods for improving the vigor of aged seeds.【Method】High temperature(40 ℃)and high humidity(95%)were used to simulate the natural aging process of the seeds, and different vigor levels of moso bamboo seeds were selected. Then, the selected seeds were primed by polyethylene glycol(PEG)and gibberellic acid(GA₃), and the physiological and biochemical characteristics of aged moso bamboo seeds were tested. 【Result】With an increased degree of aging, the relative conductivity and soluble sugar, MDA and abscisic acid(ABA)contents increased, and protective enzyme(SOD, POD, and CAT)activities and soluble protein, GA₃, indole acetic acid(IAA)and GA₃/ABA contents decreased. Furthermore, the higher the degree of seed aging, the more obvious the improvement of seed vigor was. Compared to the control, PEG and GA₃ treatments could significantly decrease the relative conductivity and MDA content to reduce the level of membrane lipid peroxidation and protect the integrity of the membrane. The two treatments could also increase the protective enzyme activities and soluble protein, GA₃, IAA and GA₃/ABA contents, as well as decrease the ABA content. 【Conclusion】The proper PEG(soaking for 24 hours with a concentration of 20% at 20 ℃)and GA₃(soaking for 12 hours with a concentration of 500 mg/L at 25 ℃)priming treatments could significantly enhance the seed vigor of aged moso bamboo seeds, and promote seed germination. These results could provide reference and technical guidance for moso bamboo seedling breeding.

引用本文

EndNote

Ris (Procite)

Bibtex

导出引用

蔡春菊,范少辉,曹帮华,赵建诚,刘凤. PEG和GA₃引发处理对老化毛竹种子理化特性的影响[J]. 南京林业大学学报（自然科学版）. 2018, 42(02): 40-46 https://doi.org/10.3969/j.issn.1000-2006.201701021

CAI Chunju, FAN Shaohui, CAO Banghua, ZHAO Jiancheng, LIU Feng. Effects of PEG and GA₃ priming on the physiological and biochemical characteristics of aged moso bamboo(Phyllostachys edulis)seeds[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2018, 42(02): 40-46 https://doi.org/10.3969/j.issn.1000-2006.201701021

中图分类号： S722

参考文献

[1] 刘娟,归静,高伟,等.种子老化的生理生化与分子机理研究进展 [J].生态学报,2016,36(16):4997-5006.DOI:10.5846/stxb201502040289. LIU J,GUI J,GAO W,et al. Review of the physiological and biochemical reactions and molecular mechanisms of seed aging [J].Acta Ecologica Sinica,2016,36(16):4997-5006.
[2] 吴聚兰,周小梅,范玲娟,等.人工老化对大豆种子活力和生理生化特性的影响 [J].中国油料作物学报,2011,33(6):582-587. WU J L,ZHOU X M,FAN L J,et al.Effects of artificial aging on seed vigor,physiological and biochemical characteristics of soybean seeds[J].Chinese Journal of Oil Crop Sciences,2011,33(6):582-587.
[3] 蔡春菊,范少辉,刘凤.毛竹种子人工老化过程中生理生化变化 [J].林业科学,2013,49(8):29-34.DOI:10.11707/j.1001-7488.20130805. CAI C J,FAN S H,LIU F.Physiological and biochemical changes of moso bamboo(Phyllostachys edulis)seeds in artificial aging [J].Scientia Silvae Sinicae,2013,49(8):29-34.
[4] COOKSON W R,ROWARTH J S,SEDCOLE J R.Seed vigour in perennial ryegrass(Lolium perenne L.):effect and cause [J].Seed Science and Technology,2001,29(1):255-270.
[5] 王彦荣,余玲,刘友良,等.数种牧草种子劣变的生活力与膜透性的关系 [J].草业学报,2002,11(3):85-91. WANG Y R,YU L,LIU Y L,et al.Relationship between seed viability and membrane permeability during seed deterioration in several forage species [J].Acta Pratacultural Sinica,2002,11(3):85-91.
[6] 毛培胜,常淑娟,王玉红,等.人工老化处理对羊草种子膜透性的影响 [J].草业学报,2008,17(6):66-70. MAO P S,CHANG S J,WANG Y H,et al.Effect of artificially aging treatment on the membrane permeability of Leymus chinensis seed [J].Acta Pratacultural Sinica,2008,17(6):66-70.
[7] KAEWNAREE P,VICHITPHAN S,KLANRIT P,et al.Effect of accelerated aging process on seed quality and biochemical changes in sweet pepper(Capsicum annuum Linn.)seeds [J].Biotechnology,2011,10(1):175-182.DOI: 10.3923/biotech.2011.175.182.
[8] TAMMELA P,SALO-VÄÄNÄNEN P,LAAKSO I,et al.Tocopherols,tocotrienols and fatty acids as indicators of natural ageing in Pinus sylvestris seeds [J].Scandinavian Journal of Forest Research,2005,20(5):378-384.DOI: 10.1080/02827580500292063.
[9] 马书燕,李吉跃,彭祚登.人工老化过程中柔枝松种子酶活性变化的研究 [J].种子,2011,30(5):9-14. MA S Y,LI J Y,PENG Z D.Study on the activity changes of enzyme in the seeds of Pinus flexilis James during artificial aging [J].Seed,2011,30(5):9-14.
[10] 张永娟,韩蕊,原烨,等.罗布麻种子老化过程中的生理生化特性 [J].草业科学,2011,28(12):2130-2135. ZHANG Y J,HAN R,YUAN Y,et al.Physiol ogical and biochemical characteristics of Apocynum venetum seed in the process of artificial aging [J].Pratacultural Sinica,2011,28(12):2130-2135.
[11] CAID H S,ECCHEMMAKH T,ELAMRANI A,et al.Alterations occurring during accelerated ageing of winter wheat [J].Cahiers Agricultures,2008,17(1):39-44.
[12] KRANNER I,CHEN H,PRITCHARD H W,et al.Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing [J].Plant Growth Regulation,2011,63(1):63-72.DOI: 10.1007/s10725-9512-7.
[13] MONCALEANO-ESCANDON J,SILVA B C F,SILVA S R S,et al.Germination responses of Jatropha curcas L.seeds to storage and aging [J].Industrial Crops and Products,2013,44:684-690.DOI: 10.1016/j.indcrop.2012.08.035.
[14] 付华,王彦荣,余玲.老化处理对几种牧草种子乙烯释放量的影响 [J].草业学报,2001,10(1):64-70. FU H,WANG Y R,YU L.Effect of aging treatment on seed ethylene production in several forage species[J].Acta Pratacultural Sinica,2001,10(1):64-70.
[15] 李胜,张真,杨广兴,等.不同处理对麻黄种子生理生化特性的影响 [J].草业科学,2007,24(1):59-64. LI S,ZHANG Z,YANG G X,et al.Effect of seed treatment on Ephedra sinica physiological and biochemical characteristics [J].Pratacultural Sinica,2007,24(1):59-64.
[16] 薄丽萍,吴震,蒋芳玲,等.不结球白菜种子活力及抗氧化特性在人工老化过程中的变化 [J].西北植物学报,2011,31(4):0724-0730. BO L P,WU Z,JIANG F L,et al.Seed vigor and antioxidant characteristics of non-heading Chinese cabbage seeds during artificial aging [J].Acta Botanica Boreali-Occidentalia Sinica,2011,31(4):0724-0730.
[17] 闫慧芳,夏方山,毛培胜.种子老化及活力修复研究进展 [J].中国农学通报,2014,30(3):20-26. YAN H F,XIA F S,MAO P S,et al.Research process of seed aging and vigor repair [J].Chinese Agricultural Science Bulletin,2014,30(3):20-26.
[18] 周芳纯.竹林培育学 [M].北京:中国林业出版社,1998. ZHOU F C.Cultivation of bamboo forest [M].Beijing:China Forestry Publishing House,1998.
[19] 江泽慧.世界竹藤 [M].沈阳:辽宁科学技术出版社,2002. JIANG Z H.Bamboo and rattan in the world [M].Shenyang:Liaoning Science & Technology Publishing House,2002.
[20] 蔡春菊,刘凤,高健,等.贮藏温度和种子含水量对毛竹种子生活力的影响 [J].安徽农业大学学报,2009,36(4):607-611. CAI C J,LIU F,GAO J,et al.Effect of storage temperature and moisture content on seed vitality of moso bamboo(Phyllostachys edulis)[J].Journal of Anhui Agricultural University,2009,36(4):607-611.
[21] 马向丽,毕玉芬,黄梅,等.不同赤霉素和聚乙二醇处理对非洲狗尾草种子萌发和幼苗的影响 [J].热带作物学报,2009,30(10):1479-1483. MA X L,BI Y F,HUANG M.Effects of GA₃ and PEG on germination of Setaria sphacelata seeds [J].Chinese Journal of Tropical Crops,2009,30(10):1479-1483.
[22] 高春智,何炎红,田有亮,等.不同浓度赤霉素浸种对樟子松种子萌发的影响 [J].内蒙古农业大学学报,2012,33(3):67-72. GAO C Z,HE Y H,TIAN Y L,et al.Effect of different concentration of gibberllin on the seed germination of Pinus sylvestris var. Mongolica Litv. [J].Journal of Inner Mongolia Agricultural University,2012,33(3):67-72.
[23] 孙刚,曹敏建,张弘,等.赤霉素、PEG对玉米种子活力的影响 [J].玉米科学,2013,21(6):73-75. SUN G,CAO J M,ZHANG H,et al.Effect of gibberellin, PEG on seed vigor of corn [J].Journal of Maize Sciences,2013,21(6):73-75.
[24] 夏方山,闫慧芳,毛培胜,等.PEG引发对燕麦老化种子活力的影响 [J].草业学报,2015,24(11):234-239. XIA F S,YAN H F,MAO P S,et al.Effects of polyethylene glycol priming on the vigor of aged oat seeds [J].Acta Pratacultural Sinica,2015,24(11):234-239.
[25] 刘凤,曹帮华,蔡春菊.GA₃提高毛竹种子活力机理探索 [J].西南林学院学报,2009,29(1):22-25. LIU F,CAO B H,CAI C J.Primary studies on seed vitality promotion mechanisms of GA₃ to Phyllostachys heterocycla [J].Journal of Southwest Forestry University,2009,29(1):22-25.
[26] 吴道藩.提高甘蓝(Brassica oleracea L. var. capitata)种子活力的方法与机理研究 [D].重庆:西南农业大学,2002. WU D F.Study on methods and mechanisms of improving vigor in cabbage(Brassica oleracea L. var. capitata)seed[D].Chongqing:Southwest Agricultural University,2002.
[27] ISTA.International rules for see testing[M].Basscrsdorf:Gurich,2013.
[28] 邹琦.植物生理学实验指导 [M].北京:中国农业出版社,2000. ZOU Q.Guide of plant physiological experiments [M].Beijing:China Agriculture Press,2000.
[29] 曹帮华,蔡春菊.银杏种子后熟生理与内源激素变化的研究 [J].林业科学,2006,42(2):32-37. CAO B H,CAI C J.Study on the after-ripening physiology and endogenous hormones of Ginkgo biloba seeds [J].Scientia Silvae Sinicae,2006,42(2):32-37.
[30] 张玲丽,郭月霞,宋喜悦.不同类型小麦品种人工老化处理后种子活力特性的研究 [J].种子,2008,27(10):52-55. ZHANG L L,GUO Y X,SONG X Y.Study on seed vigour characteristics in artificially aged wheat seeds of different types [J].Seed,2008,27(10):52-55.
[31] 邢燕,王吉庆,菅广宇,等.不同引发剂处理对西瓜种子萌发及生理特性的影响 [J].中国农学通报,2009,25(11):133-136. XING Y,WANG J Q,JIAN G Y,et al.Effect of different materials priming on the germination and physiological characteristics of aged watermelon seed [J].Chinese Agricultural Science Bulletin,2009,25(11):133-136.
[32] 李淑梅,马俊,孙君艳,等.NaCl引发处理对高羊茅老化种子萌发及幼苗生长的影响 [J].天津农业科学,2015,21(11):112-114. LI S M,MA J,SUN J Y,et al.Effect of NaCl priming on germination and growth of artificial aging seed of tall fescue [J].Tianjin Agricultural Sciences,2015,21(11):112-114.
[33] 王彦荣.种子引发的研究现状 [J].草业学报,2004,13(4):7-12. WANG Y R.Current status of seed priming research [J].Acta Pratacultural Sinica,2004,13(4):7-12.
[34] 崔路路,于海霞,李景富,等.聚乙二醇对老化甘蓝种子活力的影响 [J].北方园艺,2013(24):31-34. CUI L L,YU H X,LI J F,et al.Effect of PEG on seed vigor of aging cabbage [J].Northern Horticulture,2013(24):31-34.
[35] 王曙光,赵建奎,宁幸莲,等.PEG-6000引发对老化大豆种子膜透性及保护酶活性的影响 [J].华北农学报,2012,27(6):113-117. WANG S G,ZHAO J K,NING X L,et al.Effect of PEG-6000 priming on seed membrane permeability and the activity of protection enzyme of aging soybean [J].Acta Agriculturae Boreali-Sinica,2012,27(6):113-117.
[36] 李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响 [J].生态学报,2002,22(4):503-507. LI M,WANG G X.Effect of drought stress on activities of cell defense enzymes and lipid peroxidation in Glycyrrhiza uralensis seedlings [J].Acta Ecologica Sinica,2002,22(4):503-507.