小叶杨花粉母细胞减数分裂及花粉萌发力观测

兰月; 辛昊阳; 赵乙琏; 孙志强; 戴筱; 解志伟; 席梦利

doi:10.3969/j.issn.1000-2006.201704060

兰月,辛昊阳,赵乙琏,孙志强,戴筱,解志伟,席梦利

南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (06) : 177-180.

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (06) : 177-180. DOI: 10.3969/j.issn.1000-2006.201704060

研究简报

小叶杨花粉母细胞减数分裂及花粉萌发力观测

兰月¹,辛昊阳¹,赵乙琏¹,孙志强²,戴筱¹,解志伟¹,席梦利^1*

作者信息 +

Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr.

LAN Yue¹, XIN Haoyang¹, ZHAO Yilian¹, SUN Zhiqiang², DAI Xiao¹, XIE Zhiwei¹, XI Mengli^1*

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文章历史 +

摘要

【目的】探讨小叶杨(Populus simonii)小孢子母细胞减数分裂过程的特征及花粉萌发能力,为其杂交育种及分子细胞遗传学研究奠定基础。【方法】以小叶杨发育良好的雄花枝为研究材料,采用卡宝品红压片法,观察花粉母细胞减数分裂过程的特点,比较不同条件水培花枝对花粉萌发能力的影响。【结果】从小孢子母细胞发育到花粉粒,花芽除了稍增粗以外,外观没有明显变化; 减数分裂过程存在核仁数目的动态变化、落后染色体及不同类型的纺锤体; 室内水培花枝的花粉较室外水培的萌发能力强,室外水培的花枝花粉中观察到了2n花粉; 同一花枝上的不同花芽及同一花芽中不同小花的发育不同步,但同一小花中的不同花药发育基本一致。【结论】小叶杨可以产生天然2n花粉,是杨树多倍体育种及分子细胞遗传学研究的良好试验材料。

Abstract

【Objective】In order to lay the foundation for cross breeding and molecular cytogenetics of Populus simonii, the characteristics of the meiosis processes of microsporocytes and pollen germination ability were analyzed.【Method】We observed the meiosis processes of microsporocytes using carbol fuchin staining, and compared the pollen germination capacity under different conditions for culturing flower branches.【Result】There was no obvious change in the flower buds except slightly thicker from the stage of microsporocyte to pollen grains during the meiosis. There was dynamic change of the number of nucleolus, lagged chromosomes and different kinds of spindles during the meiosis. The germination rate for the pollen grains of indoor flower branch is higher than the pollen grains of outdoor flower branch, and 2n gametes were observed from the pollen grains of outdoor flower branch. Different flower buds in one flowering branch or different florets in one flower bud showed non-synchronization in the meiosis processes of microsporocytes, but different anthers in one floret showed synchronization in the processes of meiosis. 【Conclusion】The P. simonii could produce natural 2n pollen. It is an ideal species for the research of polyploid breeding and molecular cytogenetics of poplar.

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兰月,辛昊阳,赵乙琏,孙志强,戴筱,解志伟,席梦利. 小叶杨花粉母细胞减数分裂及花粉萌发力观测[J]. 南京林业大学学报（自然科学版）. 2017, 41(06): 177-180 https://doi.org/10.3969/j.issn.1000-2006.201704060

LAN Yue, XIN Haoyang, ZHAO Yilian, SUN Zhiqiang, DAI Xiao, XIE Zhiwei, XI Mengli. Study on meiosis of microspore mother cells and germination rate of pollen grains in Populus simonii Carr.[J]. JOURNAL OF NANJING FORESTRY UNIVERSITY. 2017, 41(06): 177-180 https://doi.org/10.3969/j.issn.1000-2006.201704060

中图分类号： S792.11

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