茉莉酸甲酯对杂交鹅掌楸体胚发育的影响

doi:10.3969/j.issn.1000-2006.201709011

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南京林业大学学报（自然科学版） ›› 2017, Vol. 41 ›› Issue (06): 41-46.doi: 10.3969/j.issn.1000-2006.201709011

茉莉酸甲酯对杂交鹅掌楸体胚发育的影响

成铁龙^1,2,孟岩^1,3,陈金慧^1,3,施季森^1,3*

1.南方现代林业协同创新中心,江苏南京 210037; 2.南京林业大学生物与环境学院,江苏南京 210037; 3. 南京林业大学林木遗传与生物技术省部共建教育部重点实验室,江苏南京 210037

出版日期:2017-12-18 发布日期:2017-12-18
基金资助:
基金项目:国家高技术研究发展计划(2013AA102705); 教育部高等学校博士学科点专项科研基金项目(SRFDP20113204130002); 江苏省高校自然科学基金项目(13KJA220001); 江苏省高校青蓝工程项目(2015); 江苏高校优势学科建设工程资助项目(PAPD) 第一作者:成铁龙(ctielong@126.com),副研究员,博士。*通信作者:施季森(jshi@njfu.edu.cn),教授。

Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid

CHENG Tielong^1,2, MENG Yan^1,3,CHEN Jinhui^1,3,SHI Jisen^1,3*

1. Co-Innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 3. Key Laboratory of Forest Genetics & Biotechnology of the Ministry of Education, Nanjing Forestry University, Nanjing 210037, China

Online:2017-12-18 Published:2017-12-18

摘要/Abstract

摘要： 【目的】探讨作为植物生长调节剂的茉莉酸甲酯(MeJA)对杂交鹅掌楸体胚发育的影响,为进一步提高杂交鹅掌楸体胚发生效率奠定基础。【方法】在已建立的杂交鹅掌楸未成熟胚体细胞发生体系的基础上,选用杂交鹅掌楸3种基因型C138、233012、253010为材料,分别添加5种浓度的茉莉酸甲酯,测定杂交鹅掌楸在添加不同浓度茉莉酸甲酯条件下的体胚发生率、成熟率、畸形胚发生率,以及再生植株率。【结果】在体胚诱导培养基中添加不同浓度的MeJA可以显著影响杂交鹅掌楸体胚发生效率,且随着MeJA浓度的增加,体胚发生率和成熟率逐渐升高,畸形胚发生率逐渐降低,而当MeJA浓度超过一定值后,体胚发生率和成熟率呈现下降趋势,畸形胚发生率也逐渐升高。杂交鹅掌楸3种基因型体胚发生率和成熟率从高到低的次序为233012、C138、253010。脱落酸(ABA)能够增强MeJA的上述效应。在体胚苗的生长阶段,浓度逐渐升高的MeJA限制了根的生长,但ABA不能改变该效应。【结论】添加1 μmol/L MeJA和2 mg/L ABA时杂交鹅掌楸体胚诱导效果最佳,此时体胚发生率和体胚成熟率相对较高,体胚畸形率最低。

Abstract: 【Object】 In order to further increase the frequency of somatic embryogenesis, the experiment was conducted to investigate the effect of methyl jasmonate(MeJA)on somatic embryogenesis development of Liriodendron hybrid. 【Methods】Based on an established system of somatic embryogenesis of Liriodendron hybrid, embryogenic cells of three genotypes included C138, 233012 and 253010 from Liriodendron chinense×L.tulipifera were cultured in the embryo induction medium with different concentrations of MeJA, and the rates of somatic embryogenesis, somatic embryo maturation, abnormal somatic embryos, and number of regeneration plants were investigated. 【Result】 The different concentrations of MeJA can significantly affect the efficiency of Liriodendron hybrid somatic embryogenesis, and with the increase of MeJA concentration, the rates of somatic embryogenesis and maturation gradually increased, the rate of abnormal embryos decreased gradually. However, when the MeJA concentration exceeds a certain value, the rates of somatic embryogenesis and maturation showed a decreased trend, and the number of abnormal embryos also increased. Of which genotype 233012 was in the first place as well as genotype C138 was higher than 253010. Abscisic acid(ABA)could enhance the above-mentioned effects of MeJA. But the increasing concentration of MeJA restricted the root growth, and ABA could not alter this effect. 【Conclusion】The induction effect of Liriodendron hybrid somatic embryos could be optimal when adding 1 mol/L MeJA and 2 mg/L ABA, the rates of somatic embryogenesis and somatic embryo maturation were relatively high, with the lowest rate of abnormal embryos.

中图分类号:

S722.8

成铁龙,孟岩,陈金慧,等. 茉莉酸甲酯对杂交鹅掌楸体胚发育的影响[J]. 南京林业大学学报（自然科学版）, 2017, 41(06): 41-46.

CHENG Tielong, MENG Yan,CHEN Jinhui,SHI Jisen. Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid[J].Journal of Nanjing Forestry University (Natural Science Edition), 2017, 41(06): 41-46.DOI: 10.3969/j.issn.1000-2006.201709011.

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茉莉酸甲酯对杂交鹅掌楸体胚发育的影响

Effects of methyl jasmonic acid on somatic embryogenesis of Liriodendron hybrid

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