茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响

doi:10.3969/j.issn.1000-2006.201712035

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南京林业大学学报（自然科学版） ›› 2018, Vol. 61 ›› Issue (06): 35-43.doi: 10.3969/j.issn.1000-2006.201712035

茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响

陈燕琼^1,2,沈 ^1,2,范佳露^1,3,陈茜^1,2,许欣玥^1,3,张迪^1,3,钱钢^1,3,陈颖^1,2*

(1.南京林业大学,南方现代林业协同创新中心,江苏南京 210037; 2.南京林业大学生物与环境学院, 江苏南京 210037; 3.南京林业大学林学院, 江苏南京 210037)

出版日期:2018-11-30 发布日期:2018-11-30
基金资助:
收稿日期:2017-12-21 修回日期:2018-07-10
基金项目:国家林业局“948”项目(2014-4-61); 江苏高校优势学科建设工程资助项目(PAPD); 南京林业大学大学生创新训练计划项目(2016NFUSPITP002)
第一作者:陈燕琼(249589249@qq.com),负责结果分析与论文撰写。沈(2276582506@qq.com),负责数据分析与部分论文撰写。*通信作者:陈颖(chynjfu@163.com),教授。

The effects of methyl jasmonic acid on leaf antioxidative capacity of Ilex verticillata L. seedlings under drought and re-watering

CHEN Yanqiong^1,2, SHEN Yang^1,2, FAN Jialu^1,3, CHEN Xi^1,2, XU Xinyue^1,3, ZHANG Di^1,3, QIAN Gang^1,3, CHEN Ying^1,2*

(1. Co-Innovation Center for the Sustainable Foresity in Southern China, Nanjing Forestry University, Nanjing 210037, China; 2. College of Biology and Environment, Nanjing Forestry University, Nanjing 210037, China; 3. College of Forestry, Nanjing Forestry University, Nanjing 210037, China)

Online:2018-11-30 Published:2018-11-30

摘要/Abstract

摘要： 【目的】落叶型冬青(Ilex verticillata L.)是一种重要的冬季观果树种,研究其干旱可为扩大其种植范围和提高其适应性提供参考。【方法】以引种落叶冬青扦插苗为试验材料,通过对照、500 μmol/L MJ(茉莉酸甲酯)、干旱、干旱+500 μmol/L MJ(drought+MJ)处理,研究外施MJ、干旱胁迫及复水条件下对落叶冬青叶片光合色素、活性氧、抗氧化水平的影响。【结果】干旱处理第12天时,叶片含水量、叶绿素和类胡萝卜素的含量都显著下降; 而干旱+MJ处理能够显著地提高上述指标的水平。干旱显著地增加了叶片H₂O₂、O^·-₂、丙二醛(MDA)的含量,在处理的第12天都分别达到最高点,而添加MJ能够在第8～12天有效降低上述指标的水平。干旱对不同抗氧化酶及抗氧化物产生不同的影响: SOD活性先下降后恢复到对照水平,POD、APX 、GR酶活性及抗氧化物质AsA、GSH含量随处理时间的延长而增加,分别在处理的第8天(GR与GSH)或第12天(POD、APX与AsA)达到高点; 而drought+MJ处理的POD、APX与AsA在第8天、GR与GSH在第3天分别达到高点,以后随着H₂O₂、MDA和O^·-₂含量的降低,POD、APX、GR活性和AsA和GSH含量也相应地处于较低水平。干旱复水6 d时落叶冬青苗仍有干旱症状,而drought+MJ处理在复水6 d时各指标基本能恢复到对照水平。【结论】连续12 d的干旱使落叶冬青苗木活性氧的含量显著地增加,造成了氧化胁迫; 添加MJ后能使其抗氧化保护系统的功能迅速开启,清除活性氧,降低氧化胁迫水平,有效缓解干旱胁迫带来的伤害,提高落叶冬青苗的抗旱性。本研究为扩大落叶冬青苗木的种植范围和提高其适应性提供参考。

Abstract: 【Objective】Ilex verticillata L.(winterberry, North America holly)is a deciduous shrub and dioecism, and also an important ornamental and commercial species owing to its red fruits in winter. The study of drought treatment to Ilex verticillata seedlings will provide reference for expanding this species scope of cultivation area.【Method】The effects of methyl jasmonate(MJ)on antioxidative capacity in the leaves by spraying MJ, drought and re-watering to seedlings including control, 500 μmol/L MJ, drought, 500 μmol/L MJ +drought(drought+MJ)treatments were investigated. 【Result】Drought significantly reduced leaf water content,pigment(chlorophyll a, b and carotenoids)content while foliar spray with methyl jasmonate under drought increased all above index levels compared to the control. Drought signifi-cantly increased the contents of H₂O₂, superoxide radical(O^·-₂)and malondialdehyde(MDA), and attained to high peak on the 12th day treatment, respectively, while application MJ significantly reduced the levels of these parameters under drought stress. Drought affected antioxidative enzyme activities and antioxidant contents differently: superoxide dismutase(SOD)activity was observed decreasing firstly then increasing, however peroxidase(POD), ascorbate peroxidase(APX), glutathione reductase(GR)activities and AsA or GSH content were increased with the drought time extension, and achieved to high peak at 8th day(GR and GSH)or 12th day(POD, APX and AsA), respectively. On the other hand, application MJ under drought stress, POD, APX activities and AsA content at the 8th day, GR activity and GSH content at the 3th day reached high peaks,respectively. Afterward, these antioxidative enzymes and antioxidants correspondingly restored to the low levels with H₂O₂, and O^·-₂ level decreasing. Ilex verticillata seedlings could recover under re-watering for 6 days after Drought+MJ treatment for 12 days, but the opposite result was found under single drought treatment. 【Conclusion】 The application MJ could early and quickly activate the antioxidant protection systems, reduce oxidative stress, relieve the injury of the drought stress and improving drought tolerance of Ilex verticillata seedlings.

中图分类号:

S725
Q945.78

陈燕琼,沈,范佳露,等. 茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响[J]. 南京林业大学学报（自然科学版）, 2018, 61(06): 35-43.

CHEN Yanqiong, SHEN Yang, FAN Jialu, CHEN Xi, XU Xinyue, ZHANG Di, QIAN Gang, CHEN Ying. The effects of methyl jasmonic acid on leaf antioxidative capacity of Ilex verticillata L. seedlings under drought and re-watering[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 61(06): 35-43.DOI: 10.3969/j.issn.1000-2006.201712035.

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茉莉酸甲酯对干旱及复水下落叶冬青苗叶片抗氧化水平的影响

The effects of methyl jasmonic acid on leaf antioxidative capacity of Ilex verticillata L. seedlings under drought and re-watering

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