崖柏属5种植物叶片挥发油成分分析

doi:10.3969/j.issn.1000-2006.201805041

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南京林业大学学报（自然科学版） ›› 2018, Vol. 61 ›› Issue (06): 179-185.doi: 10.3969/j.issn.1000-2006.201805041

崖柏属5种植物叶片挥发油成分分析

倪妍妍^1,2,张玉婷¹,刘建锋^1*,肖文发²,黄跃宁¹,姚宁¹,胡军³

(1.中国林业科学研究院林业研究所,国家林业局林木育种重点实验室,北京 100091; 2. 中国林业科学研究院森林生态环境与保护研究所,国家林业局森林生态环境重点实验室,北京 100092; 3. 南昌市林业科学研究所,江西南昌 330004)

出版日期:2018-11-30 发布日期:2018-11-30
基金资助:
收稿日期:2018-05-16 修回日期:2018-07-12
基金项目:国家林业局“948”项目(2013-4-47); 中央级公益性科研院所基本科研业务费专项项目(CAFYBB2018SY002)
第一作者:倪妍妍(nyy_ecology@126.com),博士生。*通信作者:刘建锋(Liujf2000cn@163.com),副研究员,博士。

Comparison of chemical constituents in volatile compounds from leaves of five Thuja species

NI Yanyan^1,2, ZHANG Yuting¹, LIU Jianfeng^1*, XIAO Wenfa²,HUANG Yuening¹,YAO Ning¹, HU Jun³

(1. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China; 2. Key Laboratory of Forest Ecology and Environment of State Forestry Administration, Research Institute of Forest Ecology, Environment and Protection, Chinese Academic of Forestry, Beijing 100092, China; 3. Nanchang Institute of Forestry, Nanchang 330004, China)

Online:2018-11-30 Published:2018-11-30

摘要/Abstract

摘要： 【目的】通过比对分析崖柏属5种植物叶片挥发性成分化合物的组成及相对含量,为进一步开发利用崖柏属植物资源提供理论依据。【方法】选择长势一致的4年生幼苗,通过固相微萃取技术(SPME)对幼苗相同部位叶片中的挥发性成分进行提取,利用气相色谱质谱联用(GC-MS)进行分离并鉴定,采用质谱进行定性,最终通过峰面积归一化法求得各组成成分的相对含量。【结果】5种植物幼苗叶挥发油成分共解析出136种化合物,崖柏、朝鲜崖柏、日本香柏、北美香柏、北美乔柏幼苗叶挥发油成分中分别鉴定出41、48、40、41和36种化合物。崖柏属5种植物叶片挥发油成分主要为萜类化合物,以单萜类化合物为主,质量分数均值为61.154%,其中北美乔柏含量最高(78.669%),朝鲜崖柏含量最低(49.451%)。5种植物叶片挥发油组成成分及相对含量差异较大。崖柏属5种植物共有成分仅有γ-松油烯、α-崖柏酮和(8β,13β)- 13-methyl-17-norkaur-15-ene 3种,异松油烯、α-石竹烯、棕榈酸甲酯和α,α-4-三甲基-3-环己烯-1-甲醇乙酸酯由其中4种植物共有; 且共有成分相对含量在种间差异显著,但α-崖柏酮在5种植物中相对含量均较高。崖柏属5种植物所含特有成分达75种,崖柏、朝鲜崖柏、日本香柏、北美香柏和北美乔柏所含特有成分分别为18、23、11、12和11种,且分别以长叶烯(Longifolene, 4.950%)、乙酸桃金娘烯酯(myrtenyl acetate,2.759%)、松油烯(1-methyl-4-(1-methylethyl)- 1,3-cyclohexadiene, 0.678%)、β-蒎烯(β-pinene, 4.232%)和草蒿脑(estragole,0.963%)含量最高。【结论】崖柏属5种植物挥发油化学成分以单萜类化合物为主,化合物成分组成及其相对含量差异显著,但α-崖柏酮含量均较高。

Abstract: 【Objective】 To provide a theoretical basis for the development and utilization of Thuja resources, the composition of essential oils from leaves of five Thuja species were analyzed. 【Method】 Healthy 4-year-old seedlings, having the same size and with consistent growth patterns, of five Thuja species were selected as study materials. The volatile components of new leaves in the same position of seedlings from all five species were extracted by solid-phase micro-extraction(SPME), separated and identified by gas chromatography-mass spectrometry(GC-MS). Qualitative analysis was carried out by mass spectrometry and the relative contents of the volatile components were calculated by peak area normalization. 【Result】 One hundred and thirty-six essential oils were detected; 41, 48, 40, 41 and 36 were identified from Thuja sutchuenensis, T. koraiensis, T. standishii, T. occidentalis and T. plicata, respectively. The volatile components of all five species were dominated by monoterpenes, and the average relative monoterpene content was 61.154%; the highest and lowest contents were found in T. plicata (78.669%)and T. koraiensis(49.451%), respectively. The composition and the relative content of the essential oils were significantly different among the five congeneric species. Only three components, γ-terpinene, α-thujone and(8β,13β)-13-methyl-17-norkaur-15-ene, were shared by all five species. Terpinolene, α-caryophyllene, methyl hexadecanoate, and α,α,4-trimethyl-3-cyclohexene-1-methanol, acetate, were found in four species. The common components of the essential oils differed among the five species, and only α-thujone, which was generally present at high levels, was found in all Thuja species. Seventy-five components were only identified in only one of the five species; 18, 23, 11, 12 and 11 unique components were found in T. sutchuenensis, T. koraiensis, T. standishii, T. occidentalis and T. plicata, respectively, and longifolene(4.950%), myrtenyl acetate(2.759%), 1-methyl-4-(1-methylethyl)-1,3-cyclohexadiene(0.678%), β-pinene(4.232%), and estragole(0.963%)were the unique components with the highest content in each species, respectively. 【Conclusion】 The volatile components of five Thuja species were dominated by monoterpenes, and the relative contents of essential oils from leaves differed significantly among the five species, but the α-thujone content was the highest in all five species.

中图分类号:

Q791.37

倪妍妍,张玉婷,刘建锋,等. 崖柏属5种植物叶片挥发油成分分析[J]. 南京林业大学学报（自然科学版）, 2018, 61(06): 179-185.

NI Yanyan, ZHANG Yuting, LIU Jianfeng, XIAO Wenfa,HUANG Yuening,YAO Ning, HU Jun. Comparison of chemical constituents in volatile compounds from leaves of five Thuja species[J].Journal of Nanjing Forestry University (Natural Science Edition), 2018, 61(06): 179-185.DOI: 10.3969/j.issn.1000-2006.201805041.

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崖柏属5种植物叶片挥发油成分分析

Comparison of chemical constituents in volatile compounds from leaves of five Thuja species

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