南京林业大学学报（自然科学版）

南京林业大学学报（自然科学版） ›› 1980, Vol. 4 ›› Issue (03): 104-107.doi: 10.3969/j.jssn.1000-2006.1980.03.012

• 研究论文 • 上一篇    下一篇

加龙杨(Poqulus nigra cv.Blanc de garonne)茎尖组织培养及其同工酶的变化

陈道明;黄敏仁   

  • 出版日期:1980-09-18 发布日期:1980-06-18

CULTURE OF THE APICAL TISSUE OF POPULUS NIGRA CV. BLANC DE GARONNE AND THE VARIATION OF THEIR ISOENZYMES

Chen Daoming and Huang Minren   

  • Online:1980-09-18 Published:1980-06-18

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摘要： <正>采用H培养基附加适量的2,4—D(2—3毫克/升)及BA(0.5毫克/升),诱导杨树茎尖愈伤组织的效果较好。 在低浓度BA(0.1毫克/升)及NAA(0.1毫克/升)的分化培养基上,茎叶很快分化。在愈伤组织形成幼小植株的过程中,同工酶酶谱有明显的变化。

Abstract: It was found that by using an H-medium as a basis medium with suitable amount of 2,4-D (2-3 mg/1. ) and BA (0.5mg/l), better effects were obtained in inducing cellus formations of poplar shoot apices.Stem and leaves differentiated rapidly on the differentiation medium with low concentration of BA (0,lmg/l) and NAA (0.1mg/1).In the process of plantlet formation from cellus, isoenzyme patterns changed apparently.

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