南京林业大学学报(自然科学版) ›› 1991, Vol. 15 ›› Issue (04): 20-24.doi: 10.3969/j.jssn.1000-2006.1991.04.004

• 研究论文 • 上一篇    下一篇

褐刺蛾CPV基因组凝胶电泳分析

周性恒;沈苓;夏春胜   

  1. 南京林业大学;南京林业大学;南京林业大学
  • 出版日期:1991-12-18 发布日期:1991-08-18

ANALYSIS OF GENOME OBTAINED FROM THOSEA POSTORNATA CPV BY PAGE

Zhou Xingheng Shen Ling Xia Chunsheng   

  1. Nanjing Forestry University
  • Online:1991-12-18 Published:1991-08-18

摘要: <正>褐刺蛾CPV基因组RNA在1%琼脂糖凝胶电泳中,可分离出9个片段,在3%聚丙烯酰胺凝胶电泳中分离出10个片段,各片段的大小为0.34~2.55×10~6,总分子量为15.15×10~6,其PAGE图型与CPV属代表种家蚕(Bm)CPV RNA有较大差异。试验证明褐刺蛾CPV与家蚕CPV属不同的类型。

Abstract: Double-Stranded RNA preparation have been analyzed by polyacrylamidc gel elcctrophoresis. Ten segments were recognized constitucntly for CPV. The presence of such genome segments to be a common characteristic of double-stranded viral RNA. The size distribution of the each segments is specific. The molecular weight of a segments ranged from 0.34x106 to 2.55 x 106. The sum of molecular weights of a set of the fragments were 15.15xl06.