Abstract: The structure of ecoboumdary layer and its function in the physiological activities of plants are further revealed through the analysis of the time-space variations of turbulent fluxes and their vertical gradients. In a dense tea plantation, the source and sink of material and energy are located in a part where branchlets and leaves are so crowded together as to be recognized as an entity or ecobody with specific functions when an investigation is made in a larger scale. Thickness of an ecoboundary layer is a distance between a plant and its physical environments within which they interact directly and effectively, Whereas beyond this limit no more direct interaction exists. For a tea plantation, thethickness of ecoboundary layer (D) can be approximately evaluated by the equation D=(BA) , Where HC is canopy height, (BA) is function of physiologicel activities of plants. In the case of =1, = 1, D will vary between 0. 08HC-0.16HC which is a valuable reference for investigating the environment of an ecosystem.