南京林业大学学报(自然科学版) ›› 2000, Vol. 24 ›› Issue (02): 60-62.doi: 10.3969/j.jssn.1000-2006.2000.02.018

• 研究论文 • 上一篇    下一篇

荧光假单胞菌产木聚糖酶的纯化和性质

苏玉萍;王书翰;欧凯   

  1. 南京林业大学化学工程学院;南京210037;南京林业大学化学工程学院;南京210037;南京林业大学化学工程学院;南京210037
  • 出版日期:2000-04-18 发布日期:2000-04-18

Purification and Properties of Xylanase from Pseudomonas flu.

Su Yuping\ Wang Shuhan\ Ou Kai   

  1. College of Chemical Engineering Nanjing Forestry University\ Nanjing 210037
  • Online:2000-04-18 Published:2000-04-18

摘要: <正>用硫酸铵分级沉淀 ,葡聚糖凝胶分离技术 ,对荧光假单胞菌 (Pseudomonasflu .)所产的半纤维素酶进行分离纯化 ,得到单一组分的木聚糖酶。通过凝胶电泳法 ,测得其摩尔式量为330 0 0。研究表明 ,该酶的最适pH值为 6 0 ,最适温度 60℃。酶的动力学研究测出米氏常数Km 值为 4 76mg/mL

Abstract: Using the systematical process for isolation and purification of xylanase from Pseudomonas flu. with the tehnique (NH) 2SO 4 fractionation and Sephede x G100 gel column, we obtained the xylanase showing homogeneity in the gel electrophorosis.The melecular weight was 33?000 by SDS polyacrylamide.The optimum pH,temprature were 6.0 and 60?℃,respectively.The studies of enzyme kinetics showed K m=4.76?mg/mL.

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