Abstract: Fifty five S.sapinea isolates were collected on different conifers from 13 provinces in China.Variation of sensitivity to fungicide chlorothalonil within the isolates was measured and evaluated on the basis of relative conidium germination (RCG)and relative mycelium growth (RMG).The results showed that conidia of S.sapinea were more sensitive to chlorothalonil than mycelia,and the variale range of sensitivity in conidia among isolates was also larger (RCG?1%～100%)than that in mycelia(RMG?78.4%～96.4%).It suggested that conidia sensitivity to chlorothalonil had low correlation with geographic sources, hosts and disease incidence, but mycelia sensitive to chlorothalonil seemed to have some relation with losts and disease incidence.