Abstract: Upper branches of each of 12 28-year-old trees of Chinese fir were grafted onto root systems of one-year-old seedlings as donors for rejuvenation. Basal sprouts of the same trees were rooted as donors as controls. Sprouts of these donors were cut in earth, which allows new sprouts to develop their own roots. Cutting process were done several times and then new shoots were taken as explants for tissue culture in a group. In addition, upper branches and basal sprouts were taken directly from each of 10 20-year-old trees as explants and cultured in-vitro as second group. A total of 9 subcultures were conducted. Paired sample T test method was used to compare the difference between the two explants of the same trees. Independent sample T test method was used to determine the difference between the two groups. It was found that the average number of buds and shoots per explant, the average shoot length, the rooting percentage and average root length of the upper branch explants were lower than those of the grafted explants at 0.01 level. The differences of these characteristics between graft-origin and basal-sprout explants remained in the first 3 subcultures, however, they disappeared in the late. The results indicate that grafting scion, cutting stems, rooting and tissue culture itself have some functions of rejuvenating mature tissues of Chinese fir.