Abstract: In this paper,techniques for induction of callus and cell culture in Magnolia officinalis have been studied,with an aim to establish a cell line in which cells proliferated fast and phenol content was high.Based on tissue culture and the establishment of a high yield cell line of 10 genotypes from a provenance test forest of Magnolia officinalis ,it shows that such factors as basic media,types and proportions of hormones,and explants had a great effect on the induction rate,growth and browning of the callus.With a medium of B 5 +4 mg/L 2,4 D+1 mg/L NAA,the induction rate reached 100%.With the medium of B 5 +1.2-2.0 mg/L BA+1.0 mg/L NAA,the callus had a highest proliferation rate and a lowest percentage of browning.Cells of the two genotypes, viz.140 and 54 grew fastest and were 2.12 times as many as those of other genotypes on the average.The content of phenols differed between calluses cultured from different genotypes and media as well as for a different period of time.The best one was the cell line of the genotype 140 cultured on the medium of B 5 +2.0 mg/L BA+ 1.0 mg/L NAA for 60 days.