Abstract: 14 strains Acacia rhizobia were isolated from different Acacias growing different stands,and Acacia crassicarpa seedlings were inoculated with those Acacia rhizobia.The results showed that the indigenous rhizobia were better than the control L1,the seedlings height growth inoculated with Hei 1,Ma 6,Hou 5 and Zhi 1 strains were increased 4.5%～18.6% more than that of the control L1,the seedlings diameter growth inoculated with the indigenous rhizobia were increased 2.5%～46.8% more than that of the control L1 except Hou 1,Za 2 and Tai 1,the nitrogenase activities that inoculated with Zhi 4,Ma 5,Za 1 and Ma 6 strains were increased 7.6%～241.8% more than that of the control L1,the chlorophyll contents of the seedlings inoculated with Zhi 1,Ma 5,Ma 3,Ma 6,Hou 1,Za 1 and Hou 8 were increased 11.0%～19.1% more than that of the control L1,Nitrate reductase activity of seedling leaves inoculated with Hou 6,Tai 1,Ma 3,Zhi 1,Ma 6 and Zhi 4 were increased 3.3%～34.4% more than that of the control L1,the N contents of the seedling leaves inoculated with Za 2,Zhi 4,Hou 6,Hou 8 and Hei 1 were increased 7.4%～43.8% more than that of the control L1,the P contents of the seedling leaves inoculated with indigenous rhizobia except Za 1 were increased 9.1%～72.7% more than that of the control L1,the K contents of the seedling leaves inoculated with Ma 3 were increased 8.3% more than that of the control L1,the Ca contents of the seedling leaves inoculated with indigenous rhizobia except Hou 1,Hou 6 and Hou 8 were increased 7.3%～41.5% more than that of the control L1,the Mg contents of the seedling leaves inoculated with indigenous rhizobia except Hou 1,Hou 6,Hou 8 and Hei 1 were increased 12.5%～25.0% more than that of the control L1,the Fe contents of the seedling leaves inoculated with Hou 1,Ma 5,Ma 6,Za 1,Za 2 and Tai 1 were increased more 10.0% than that of the control L1.