Abstract: Seven mitochondrial DNA regions of Anoplophora glabripennis and its sibling species are sequenced to understand the molecular characteristics of mtDNA.The results show that the base variation of the seven mtDNA gene sequences of Anoplophora glabripennis and those of its sibling species is above 11.3%,whereas the base variation among Anoplophora glabripennis species group and its individuals is less than 3%,nearly no variation existed in most genes,these character imply that these mitochondrial genes are conserved intraspeciesly and could be used in identification and phylogenetic analysis of Anoplophora glabripennis.The study also suggest that Anoplophora glabripennis and A.nobilis might belong to same species as their sequence variation in these genes are negligible.In the sequencing of COⅠ,Cytb genes,sequences with significant variation are found in some individuals,sometimes they are similar to those of the sibling.It remains to be elucidated that weather this is due to the presence of nuclear pseudogene,or it may arise from mitochondrial inleakage.