Abstract: According to the rooting methods of nonrooting seedling in vitro,longer than 3 cm of Robinia pseudoacacia f. decaisneana and the cultivar of No. 1, No. 2, No. 3 of Actinidia arguta of nonrooting seedling in vitro were selected for the root-inducing tests. The root-inducing treatments were conducted by the root-inducing regulators before and. after transplant of nonrooting seedling in vitro in order to make nonrooting seedling.rooting in the field directly. The optimum growth condition were temperature(26±2)℃ and humidity 85% above. Before transplant treatments the nonrooting seedlings dipped the basal part of seedling stem with root-inducing regulators and transplanted in the No. 6 of mixture bases. After transplant treatments the nonrooting seedlings were watered root-inducing regulators of N+T one time only and one time weekly for three weeks. The different test results showed that after transplanted the nonrooting seedlings were watered three times/one time weekly by use of the optimum prescription of root-inducing regulators of N+T(2.5～3.5 mg/L). Their survival rate of t.ransplanted seedlings were R. pseudoacacia 86.8% and A. arguta 97.8% separately. The test results showed that the treatments could increase the survival rates of transplanted nonrooting seedlings in vitro of R. pseudoacacia and A. arguta significantly by being watered three times/one time weekly by use of the optimum prescription of root-inducing regulators.