<正>连续3年调查发生慢性枯萎病不同发病程度的毛竹，进行了病原的分离、纯化及鉴定。结果表明，毛竹慢性枯萎病的病原菌是一种粘帚霉（Gliocladiumsp.）。该茵初生分生孢子梗轮枝状，长96～128,um，顶端轮生4～5个细瓶形小梗，轮生小梗长约19.2～24μm；次生分生孢子梗青霉状，长64～128μm，瓶梗大小（4.0～4.8）μm×（6.4～9.6）肛m；分生孢子大小（2.4～6.2）FmX（1.9～3.2）μm。

As an effective tool for the research on gene discovery and gene expression profile, expressed sequence tags (EST) have been taken into account by many laboratories in the world. Reducing the cost of large-scale EST sequencing and improving the success rate of EST sequencing are particularly important for relevant research. Based on different comparison between such factors as response System,BigDye type, content of BigDye,purification of sequence product and 96-well reaction plate,the relative cheap project of large-scale EST sequencing has been confirmed. The quality of cDNA library and other influence factors have been discussed at the same time. It is significant and useful for reducing the cost and improving the success rate of EST sequencing.